LOCUS       sequence-30968-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Yan N, Cherepanov P, Daigle JE, Engelman A, Lieberman J
  TITLE     The SET complex acts as a barrier to autointegration of HIV-1.
  JOURNAL   PLoS Pathog. 2009 Mar . 5(3):e1000327.
  PUBMED    19266025
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Mar . 5(3):e1000327."
COMMENT     Sequence Label: pcDNA-SETin-FLAG-HA
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..44)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(18..44)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(51..71)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(78..101)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagactcga gctattaggc gtagtcgggc acgtcgtagg ggtatccggc gccctgaaaa
       61 taaagattct catcgagctt gtcatcgtcg tccttgtagt cctgttggtc atcttctcct
      121 tcatcctcct ctccttcctc cccttcatca tcatcttcat cttcttcacc ttcatcctca
      181 tccccttctt cgtcaatatc ttctaatcct tcctcctctt catcatcatc atcatcttct
      241 tctccttctc cttcttcatc atccatatcg ggaaccaagt agtactgtaa tgggtttggc
      301 caaatatcat ctttgatgac ctctcctaac tcatcagcac ctgcatcaga atggtcagta
      361 aaccaggtaa agaagctctc tggttcctca tgctgcctct tcctgctggc tttattctgc
      421 gtttgactcg aacgtttcgt caaatccttt ccagatttcc atttgatttc ggtggacttc
      481 gaagatggat caccactctc attcagatga aattctttgg agagaacttt attttcaaag
      541 taaggatttt catcaaaata aaaatctatt ctgtaacctg atttaatatc ttcaaattct
      601 gtcacttcaa ctctggtcaa ataatgcagt gcctcttcat cttcctcccc aagcagtgca
      661 gacacttgtg gatggttgac aaatgttgtt acccaaaaat ttgggatttt ggcgatcaat
      721 tctgacctct tctgaaaaaa tggttggcgg agtttgttat atttctgttc tactttcaaa
      781 atctcctcac tggcttgttc attaagtctg tctatttcat tttgtacttc atcaatgtgt
      841 tcaatcgctt cttgctgttc tttctcagac gtt
//
