LOCUS       sequence-309691-        6082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Nishimura M, Isaka F, Ishibashi M, Tomita K, Tsuda H, Nakanishi S, 
            Kageyama R
  TITLE     Structure, chromosomal locus, and promoter of mouse Hes2 gene, a 
            homologue of Drosophila hairy and Enhancer of split.
  JOURNAL   Genomics. 1998 Apr 1;49(1):69-75.
  PUBMED    9570950
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genomics.";
            date: "1998-04-1"; volume: "49(1)"; pages: "69-75"
COMMENT     Sequence Label:  pHes1(467)-luc
FEATURES             Location/Qualifiers
     source          1..6082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1653
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(67..85)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          1894..1959
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2089..2109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2534..2668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2810..2827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2981..3569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3061..3080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3740..4531,4532..4600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4363..4382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4601..4705)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4732..5187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4819..4838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5029..5050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5377..5498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt
      361 tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga
      601 tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac
      841 aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg
      961 aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct
     1321 ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa
     1381 caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa
     1681 attcttagct attgtaatac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1741 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
     1801 atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     1861 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     1921 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     1981 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     2041 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     2101 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     2161 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     2221 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     2281 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     2341 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     2401 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     2461 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2521 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2581 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2641 catcaatgta tcttatcatg tctggatccg tcgaccgatg cccttgagag ccttcaaccc
     2701 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     2761 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     2821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3421 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     4741 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4801 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4861 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4921 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4981 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5041 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5101 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5161 ttaacaaaat attaacgctt acaatttgcc attcgccatt caggctgcgc aactgttggg
     5221 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa
     5281 gtaatattaa ggtacgtgga ggttttactt gctttaaaaa acctcccaca cctccccctg
     5341 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     5401 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5461 tctagttgtg gtttgtccaa actcatcaat gtatcttatg gtactgtaac tgagctaaca
     5521 taacccaatt ccggaagaca ttagagtaac acatcctctt taccttgttc cctccttttt
     5581 tcaatcacta aattttgtct tggcctatat ctgttcaaaa tatttttcaa atgaacttat
     5641 tatacaaagt agttatattg catgcagcaa gaacaataaa aaccaaaggc ctggccacaa
     5701 aagaaataga ctagactaaa actaagcaaa gcccagagga aagagttagc aaagggttaa
     5761 aatccttttg attgacgttg tagcctccgg tgccccgggc tcaggcgcgc gccattggcc
     5821 gccagacctt gtgcctagcg gccaatgggg gggcgcagtc cacgagcggt gccgcgtgtc
     5881 tcttcctccc attggctgaa agttactgtg ggaaagaaag tttgggaagt ttcacacgag
     5941 ccgttcgcgt gcagtcccag atatatatag aggccgccag ggcctgcgga tcacacagga
     6001 tctggagctg gtgctgataa cagcggaatc ccctgtctgg tcgagatcta agtaagcttg
     6061 gcattccggt actgttggta aa
//
