LOCUS       sequence-309712-        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 
            without its STOP codon .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Marchal C, Wheat Genome Project, Haberer G, Spannagl M, Uauy C
  TITLE     Comparative Genomics and Functional Studies of Wheat BED-NLR Loci.
  JOURNAL   Genes (Basel). 2020 Nov 26;11(12). pii: genes11121406. doi: 
            10.3390/genes11121406.
  PUBMED    33256067
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2020-11-26"; volume: "11(12). pii: genes11121406. 
            doi"; pages: " 10.3390/genes11121406"
COMMENT     Sequence Label: pUAP1_Ta_Yr7_CDS_no_STOP
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..58
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(82..99)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(253..841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(333..352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1024..1118)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1139..1798)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1713..1732
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1960..1978
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1982..2025)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
       61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
     1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcggtctca
     2041 aatggagccg gcgggagact cttccctgga ggccgcgatt gcatggctgg tgcagaccat
     2101 ccttgcaacg ctcctcatgg acaagatgga ggcctggatt cagcaagtcg ggcttgccga
     2161 cgacgtcgag aggctccagt ctgaggtcga gagagtcgac acggtggtgg ctgctgtgaa
     2221 ggggagggca gccgggaaca tgcctctgtc ccggtcactc gctcgtgtca aggagcttct
     2281 ctatgacgcc gacgacgtga tcgacgagct agactactac aggctccaac accaagtcga
     2341 aggagttaca agtgacgagc ctgacggtat gcgtggagct gaaagagtgg atgaaatatc
     2401 aaggggccat gtcgatacac tgaatgtcag tgttggcaaa ttacggtccc cggtatggga
     2461 acacttcacc atcacagaaa caactatcga cgggaagcgt tcaaaagcca aatgtaagta
     2521 ctgtggaaat gattttaatt gcgaaacgaa aacaaacggg acttcatcta tgaaaaaaca
     2581 tttggagaag gagcattccg tgacttgcac gaataaatct gcagtgcacc ccccaaacac
     2641 ttcaagcacc ggcgatgcta cttgtaatgt gaggtcggtt gaagttggta gttcgtccaa
     2701 cggaaaaaga aagagaacaa atgaggatcc aacgcagacc accgcagcta acatacacgc
     2761 ccaatgggac aaggctgagt tatccaatag gataattaaa attactgaga agttacagtt
     2821 acaggacatc cagggggctt tgagtaaagt tctcgagcca tatggatcca gcgctacttc
     2881 aagttcaaat catcaccgct tgagtacagc atcagatcag cacccaacaa catcaagtct
     2941 tgttccaatg gaagtttatg gcagagttgc agaaaagaat aagatcaaaa agtcaataac
     3001 tgaaaaccaa tctggtggtg taaatgttct gcctattgta ggcattgcag gtgttggaaa
     3061 gacaactctt gctcaatttg tgtataatga tccagacgtg aaaagtcaat ttcaccacag
     3121 gatatgggtt tgtgtgtcct gcaaatttga tgaagtgaag ctcacaaagg agatgttaga
     3181 cttttttcct cgagaaaggc atgaaggaat taacaacttc gcgaagcttc aagagatctt
     3241 gaaagaacat gtcgagtacc aagcaaagag ttttctgctc attttagatg atgtctcgga
     3301 cagtatggat tatcataaat ggaacaaatt gttgaaccct ttgctatcaa gtcaagcgaa
     3361 gaatataatt ctagtcacga ccagaaattt gtctgttgca caaaggttaa gcacacttga
     3421 accgatcaag ttaggtgctt tagaaaacga tgatatgtgg ttattgctca agtcatgtgc
     3481 atttggtttt gggaactatg aaggtacgga aaatctaagc actattggaa ggcaaatagc
     3541 agagaagtta aagggcaatc cgttagcagc agtaactgca ggggcactgt taagagataa
     3601 tcttagcatt gatcattgga gtaacattct caagaatgag aagtggaaat cgctgggact
     3661 cagtgggggc atcatgcctg ctttgaagct tagttatgat gagttgacgt accgtttaca
     3721 acaatgtttc tcgtattgct ctatatttcc tgacaaatat aggtttctcg ggaaggattt
     3781 ggtctatatt tggatttctc agggatttgt gaattgcacc caaaataaga gattggagga
     3841 gacgggatgg gaatatctga atcaattggt aaatcttgga ttctttcaac aaattgaaga
     3901 acaacaagaa ttggatgggg aagaagaatt ctctctacgc cgtcagattt ggtactctat
     3961 gtgtgatctc atgcatgatt tcgcaaggat gatttcaagg actgaatgtg cgactataga
     4021 tggtctacag tgcaataaaa tattcccaac tgtacagcat ttgtcaatag taaccggttc
     4081 tgcatacaac aaagatctga aggggaacat tcctcgtaat gagaagtttg aagaaaatat
     4141 gagaaattca gttacatcag ttaccaaatt gagaacattg gttgtgcttg ggaactttga
     4201 ctctttcttt gtacggttgt tccaagatat attccagaag gcacaaaatt tacgcctgct
     4261 gctagtatct ctagcatcca cttatctgtc tcaagtgcct gctgcattca atgattttaa
     4321 ttccttcctg tgcaatttgg caaatccttt gcatcttcgt tacctaaaac ttgagttgga
     4381 tgggattgtg ccacaagttt tgagtacgtt ttttcatctt caagtattag atgttggatc
     4441 aagcatggat acttctctac ccaatggctt gttgcataat cttgttagcc tgcgacatct
     4501 tgttgcacac aagagagtcc attcttccat tactagcatt ggtaacatga catctatcca
     4561 ggagctacat gattttgaag ttcgaatttc tagcggcttt gagataacac gactccaatc
     4621 catgaacgag cttgttcaac ttgggttgtc tcaacttgac agtgttaaaa ccagggagga
     4681 cgcttatggg gcaggactaa gaaacaagga acacttagaa gagcttcatt tgtcctggaa
     4741 ggatgcatat tcagagtatg agtatgccag tgacactgaa tttgaatctt ctgcaaacat
     4801 ggcaagagaa gtgattgagg gtcttgaacc acacatggat ttaaaacatc tacaaatatc
     4861 tcagtataat ggtaccactt caccagcttg gcttgccaac aatatctcag ttacctcatt
     4921 gcagacgctt catcttgatg attgtggagg atggagaata cttccatctc tgggaagtct
     4981 accattcctt acaaaggtga agttgagcag catgctggaa gtaattgaag tactgattcc
     5041 ttcactggag gagctagttc taattaaaat gccgaagtta gtgagatgct caagcacttc
     5101 tgccgagggt ctgagctcta gcttaagggt actgcacatt gaggattgtg aagcattgaa
     5161 ggagtttgat ctgtttgaga acgattataa ttctgaaatc attcagggat catggctgcc
     5221 tggtcttagg aatttgattc tatattgttg ccctcatttg aaagtgttga agcctcttcc
     5281 accttcaact accttttcta aggtactcat cagagaaatt tcaagatttc cgtctatgga
     5341 ggtatcatct ggtgagaagt tacaaattgg gaatattgat gtgtacatag gcgatgattt
     5401 tgatgagtca tctgatgagt tgagcatact ggatgacaaa actttggcgt tccataatct
     5461 tagaaacctg aaatcgatgg agatatatgg ttgcagaaat ctaaggtctt tttcgttcga
     5521 aggtttcagt catcttgtct ctttaacaag tttgaaaata gtaagctgtg aacaactttt
     5581 cccttcagat gtgacggcag agtataccct tgaagatgtg acagctgtga actgcaatgc
     5641 cttcccatat cttaaaagcc tcagtatcga ctcatgtgga atagcgggga agtggctatc
     5701 gctgatgctg cagcatgcgc caggcctaga ggaattgagt ttaacaagtt gcgcccatat
     5761 aacaagagta gtgttaccga tggaagagga agaaaacaat ctattaacaa cagtactgtc
     5821 atcaggaaat caagatgagg cattgacatg gttagttcgt gacggactct tgcacattcc
     5881 atcaaatctc gtctcctctc tcaagaatat gagtattact cagtgccctc gcctaaagtt
     5941 taactcaggc aaggactgct tctctggatt tacctcgctt gagaagcttg aaatttgggg
     6001 atcgttggtg gatgatgacg gaagtgatga cctggagaat ggaagttctt ttgtgttcgg
     6061 agaggaggat caacccctgg gggcgaacgg aagatggctc ctcccgacat cacttcagga
     6121 acttcacatc gtgtcattgt attgccaaga aacgctgcaa gtctgcttcc ctagagatat
     6181 caccagcctt aaaaagttaa gtgtacgttc cggccaaggt ttgcaatctc tacagctgta
     6241 ctcatgcacg gcactggaag aattggcaat ttccggctct ggatcggtca ccgtcactgt
     6301 actagagggc acgcaacccg ctggcagcct cgggcgtttg aatgtatcag actgtcctgg
     6361 cttgccatca cgtttggaca gctttccaag gttgtgccct cggctggaaa ggcttgacat
     6421 caatgaccca tctgtcctta ccacgccatt ctgcaagcac ctcacctccc tgcaacgcct
     6481 aaaacttggc ttcttgaaag tgacgagact aacagatgag caagaacgag cgcttgtgct
     6541 cctcaagtca ctgaaagagc tcgagatttt ttattgtact catctcatag atcttcctgc
     6601 ggggctgcag acccttcctt ccctcaagag tttgaagata gaagagggtc gaggcatctc
     6661 aaggctgccg gaagcaggcc tcccacattc gctggaagaa ctggaaatca aaatttgcag
     6721 caagctagaa gatgaatgca ggcggctagc aacatgcgaa ggcaagctaa aagtcaaaat
     6781 tgatggtcga tatgtgaatt cgcgagaccc tgcag
//
