LOCUS       sequence-309716-       19837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient plant transformation vector containing selection markers 
            and a Cas9 expression cassette, but lacking guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19837)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 19837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE347
FEATURES             Location/Qualifiers
     source          1..19837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6805..7500)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(8723..8975)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(9575..9757)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(9611..9789)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(10529..10549)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(16282..16302)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        18012..18114
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             18115..18798
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(18181..18200)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             19118..19423
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     19302..19321
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    19544..19568
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctctctgag
     6361 cgactaaatg gagcaaccta ctgtttttga agtttgggtt gccatgtaat cacttgcaat
     6421 aaatatatgt actaatgact gcatcggctt gcgtgtctaa atttgtagct tattgaaaac
     6481 tttttgtggt taaagacgaa tccagttaac taacataagt ttaacaaaac aagacctttt
     6541 ccatatatag accaccgtta ccaacttaca aagaagccaa ataaagtaca acacaacact
     6601 tgcaagttac aaaaagagag gaaaaaaaac atggtaccat ttgacaacat atagaaacac
     6661 acataaacat tacttataac aaaaagattc aaactcggct tttattatct ccccctgatc
     6721 aaattctaaa cactttattc ctcataaact aactccccga cattggagtt attggactat
     6781 gacgatgaca tcagtggggt aagctcactt gtgccccagt ttgctaggga ggtcgcagta
     6841 tctggccaca gccacctcgt gctgctcgac gtaggtttct ttgtcggcct ccttgattct
     6901 ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt
     6961 ggatctgtat gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc
     7021 catgtcgctt ctgccttcca ggccgccgtc agcggggtac agcatctcgg tgttggcctc
     7081 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat
     7141 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     7201 gcccccatct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     7261 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ctgctgccgt acatgaagct
     7321 ggtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
     7381 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt
     7441 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcat
     7501 agtagtgtgc tggccaccac gagtacggtc acggtcatgt tccccaccag tatgttgctg
     7561 tccgtagtac tgagctctgt ctttcagatc ctgagctttg cttcccaact tcatccttgc
     7621 actgtccaac ttgtctgatc cctgtgggtg ctctcccgtt gcgtacctat tcacaatcaa
     7681 taggtatcat accaagcatt ttacctatat agatgatata cacagacaca ttcaagctag
     7741 ctaggtcaaa tttcgtattt agttaaccaa atgttcccta gaatttgcaa tttcttattg
     7801 ttacatttgt tattcgacca aaaaaattga tccaaagcta ctggctcaac acatgcatgc
     7861 acatattgca caaaattatg aagtaagatg ataaatgtgt gcttacttgt aaatccaaga
     7921 gaaaacggtt atagcggcaa tgccaaaccc tccagaggaa agaaaaccgg tgatgaggag
     7981 tgcaactgtg atgagagccg ggacaaggat tgggctgaag ataacgagca gaggtgttgc
     8041 aacagtcaaa gctatgacag ttccaacaag ggtaaggctg gagagaacaa ggagggaacc
     8101 accagctgtg acagcagttg cagctttagc aatctgccta gacttggagt agtcagatcc
     8161 tcgtccggac atctggtact ggtcacgatc tcggcccatc atcgggtact gatctctgcc
     8221 gatgatatcg tgatgggttc ctctagctgt atccgccatt tttttgttct tgtttactag
     8281 agagaatgag attttgaatg agagattgat caaaaagatg tgtatatatt ttggaagagg
     8341 taggagtgtt tgttagatat attaagaaag gagacgagaa gtcacgtgtc aatggagaca
     8401 tcatgcaatt gtaggccacg tgttaatggc gtcaccatgc agccgctgca tgtgttaacg
     8461 agaggagtct attcaaagac acgtttgttt tttgcctagc tcagaatgtg gcgttggcat
     8521 cgaaccgaaa cataaaacgc aaaacgaacc tttgggctcg tcagtatggg tttgggcctt
     8581 caatagattc cttctatacg gcccacatat ccactattgg caacaaaatt agaaaaattt
     8641 aggatttagt tttaaaattt gttccgcgac attctagaac tccccatggg aattcgtaaa
     8701 gcgctccaat tcagcaagcg tcgatctagt aacatagatg acaccgcgcg cgataattta
     8761 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     8821 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     8881 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     8941 cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctagat ccagagtccc
     9001 gctcaaatct cagtcacagg aagaacaggt ctaggaggaa caggaagaga gaaatcaagc
     9061 tgccagaatc ccacatcatg ccaatttcca tgtttgaaac cagcagctct aagcattccc
     9121 ctaggagcat aaccaagagc ctcatgcatc ctcacagaag gatcattagg aagtccaatc
     9181 acagcaacca cagacttgaa accctgagcc tccaaagact tcaaaagatg agtgtaaaga
     9241 gtagatccaa gaccagttct ttgatgccta ggagacacgt aaacagtaga ctcagcagtc
     9301 caatcgtaag cattcctagc cttccaagga ccagcataag caataccagc cacttcacca
     9361 tccacttcag caacaagcca aggatacctc tctctaagcc tcacaagatc atcagtccac
     9421 tcttgtggtt cttgaggctc agtcctgaaa ttcacagtag aagtctcaat gtaatgattc
     9481 acaatagtgc acacagcagg catatcagcc tcagtagccc ttctaatatc agcaggcctt
     9541 ctttcaggag acatgcgaaa cgatccagat ccggtgcaga ttatttggat tgagagtgaa
     9601 tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag cattttgaca
     9661 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     9721 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     9781 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     9841 aacgtgactc ccttaattct ccgctccgag ctcgaattct agtagcgttg tcaatcaatt
     9901 ggcaagtcat aaaatgcatt aaaaaatatt ttcatactca actacaaatc catgagtata
     9961 actataatta taaagcaatg attagaatct gacaaggatt ctggaaaatt acataaagga
    10021 aagttcataa atgtctaaaa cacaagagga catacttgta ttcagtaaca tttgcagctt
    10081 ttctaggtct gaaaatatat ttgttgccta gtgaataagc ataatggtac aactacaagt
    10141 gttttactcc tcatattaac ttcggtcatt agaggccacg atttgacaca tttttactca
    10201 aaacaaaatg tttgcatatc tcttataatt tcaaattcaa cacacaacaa ataagagaaa
    10261 aaacaaataa tattaatttg agaatgaaca aaaggaccat atcattcatt aactcttctc
    10321 catccatttc catttcacag ttcgatagcg aaaaccgaat aaaaaacaca gtaaattaca
    10381 agcacaacaa atggtacaag aaaaacagtt ttcccaatgc cataatactc aaactcagta
    10441 ggattctggt gtgtgcgcaa tgaaactgat gcattgaact tgacgaacgt tgtcgaaacc
    10501 gatgatacga acgaaagctc taagctcaga ctttacgctt cttctttggg tcagccctag
    10561 agtcaccacc caactgcgac agatctatcc ttgtctcata gagccctgta atagattggt
    10621 gaataagggt agcgtccaac acttccttgg ttgatgtgta gcgctttctg tcgatagtgg
    10681 tatcgaagta cttaaaggct gctggggctc ccagattggt cagtgtgaac aggtggatga
    10741 tgttttccgc ttgttccctt atcggcttat ctctgtgctt gttgtacgcg gaaagcacct
    10801 ttaattgtag aaaaccagta aaacatggac aattaggagg catcgattat ctaaccaaag
    10861 actttaacaa cactaatctg ccactaatat tcatgagaag tccttacctt atcgaggtta
    10921 gcgtctgcaa gaatgacccg cttggaaaat tcgctgatct gctctatgat ctcatcaaga
    10981 tagtgcttgt gctgctcgac aaacaactgc ttctgctcgt tgtcctctgg tgaccccttc
    11041 agcttctcat agtgtgaggc caagtacagg aagttgacgt acttactggg caatgccagc
    11101 tcgttgccct tctgcagttc tccggcagat gccagcatgc gttttctgcc attttccaat
    11161 tcgaaaagcg aatacttggg cagtttgata atgaggtctt tcttcacctc tttgtaacct
    11221 ggtttgtata attttggttc agttaattag gaaaattaca taatggctcc aaaaaaaaat
    11281 tatcgacctt ttaccaaaaa aaataaaatt tatcgacctt gacaggaaaa agttacaaat
    11341 caaaagaaag aatatcgtct ggcatccaat attttacctt tagcctcgag gaagtcgatc
    11401 ggattcttct cgaacgagga cctttccatt atggtaattc caagcagttc cttgacgctt
    11461 ttcagtttct tgctcttgcc cttctccacc ttggccacta ccagtacgct gtatgccacg
    11521 gtagggctat caaagcctcc gtatttcttc ggatcccaat ccttcttcct cgcaatcagt
    11581 ttgtcggagt tgcgcttagg gagaatggac tccttggaga aaccaccggt ttgaacctcc
    11641 gttttcttga ctatgttgac ttggggcatg ctgagaacct tacgcacagt cgcaaaatcc
    11701 ctgcccttat cccagacaat ctcacctgca agaaacagca aagagagagt taagatataa
    11761 gtgaacaaga aagtcttggg atcgacatac atacaatcaa ttgatcaaat tcctaggatt
    11821 aaacatatga aataggaaag acagacctgt ttcgccattt gtctcgatca gaggcctctt
    11881 ccggatttca ccattcgcca gagtgatctc tgtcttgaag aagttcatga tattcgagta
    11941 aaagaagtat ttcgcggtag ccttgccgat ctcttgttct gacttcgcga tcatcttcct
    12001 aacgtcgtac accttgtaat ccccgtacac aaactcagac tccagtttcg gatacttctt
    12061 gatgagagca gtgccaacaa cggcgttcag gtaggcgtca tgggcatgat ggtagttgtt
    12121 gatctcgcga accttgtaaa actggaagtc cttccggaag tcactaacca gtttgctctt
    12181 gagggtgatc acctgcgtaa ccatcaaaag tcaatatgga ttcatcacaa attaatcagg
    12241 aatagaaaga tcgggtggcc ttgaagttga tcaaataaaa agtttcgaac tttagtttga
    12301 tatggtaaag gaaagttgga aactttacct ttacctccct gataagtttg tcattctcat
    12361 catacttcgt attcatacgc gaatcgagta tctgggccac atgtttcgta atctgacgag
    12421 tttcaaccaa ctggcgcttg atgaagccgg ctttgtcgag ctcagacagt ccgccccttt
    12481 ctgccttcgt gagattgtcg aatttccgtt gggttatgag cttagcattc aaaagctgcc
    12541 tccaatagtt cttcatcttc ttcacaacct cctcgctggg tacattgtcg gattttccgc
    12601 ggttcttgtc tgagcgcgta aggacctata acacgggaga aaccaaaaag caaactcatt
    12661 tcacgccaaa tactacagat ttctcaaaaa ttaagttata atccatatat cctgacaaga
    12721 aattgattaa aacacagttg ctttaccttg ttgtcaatac tatcgtcctt caggaaagat
    12781 tgagggacaa tgtgatccac atcatagtcg gagagtctgt taatgtcgag ctcttggtcg
    12841 acgtacatat ctctcccatt ctggaggtaa tagaggtaca gcttctcgtt ctggagttga
    12901 gtattttcga ctgggtgctc tttcagaatc tgactcccaa gctccttgat gccttcctct
    12961 atccgcttca tgcgctcccg tgagttcttc tgccctttct gagtcgtctg gttttccctc
    13021 gccatctcga tgacgatgtt ctccggctta tggcggccca tgacctgtag caataattaa
    13081 aacaaaaata ccacaaacag ttagaactga ggcaagaaga atgaatgcag agaaaaaaaa
    13141 tcttagctat tcatggaaat ggaagtgcga cgaacaccta aaatgcaagg agcataacca
    13201 aatgcagaac ttaccttcac cagctcatcc acaaccttca ctgtctgcag tatgcccttc
    13261 tttatagctg gcgatccagc caggttggct atgtgttcgt gaaggctatc gccctgacct
    13321 gacacttggg ccttctggat atcttctttg aaggtcagtg agtcatcgtg aataagctgc
    13381 atgaagttcc gattcgcaaa accgtcggat ttgagaaagt ccagtatagt cttgccggat
    13441 tgcttgtcgc ggatgccgtt gatgagcttc ctggagagcc ttccccaacc taaaacatag
    13501 aacaaacatt tgtctcatca caaatatgca tcaaggaaca ctcagaagtt agtcatatac
    13561 ccaaattctt gaaactagag aaacaaaaaa agagggatca ttgcggaaac cttttgacct
    13621 cttacctgta tatctcctcc tcttcaactg cttcatgacc ttgtcatcga acagatgcgc
    13681 ataagtctta aggcgctcct cgatcatttc tctgtcctca aacagcgtga gagtcagcac
    13741 gatgtcctcg aggatgtcct cgttctcctc gttgtcaagg aagtccttgt ccttgatgat
    13801 tttcaacagg tcatggtatg tgccaagaga ggcattgaac ctatcctcaa cgccgctgat
    13861 ttccacagaa tcaaagcatt cgatcttctt aaagtagtcc tctttaagct gtttcacagt
    13921 gaccttccga ttggttttga acaagagatc gacaatggcc ttcttctgct cacctgttat
    13981 aacagtaaaa attactttag aaacaaaaca aaagtgtggt cttttaggaa tgattattga
    14041 tcagttcata gtttaaaatc ttgtgttttt atcgtaaaag tgtggaccag gatttacctg
    14101 acaggaaggc cggtttgcgc atcccctccg tcacatattt caccttcgtc agctcgttgt
    14161 agaccgtgaa gtattcgtac aggagactat gcttgggcag gactttctca tttgggagat
    14221 tcttatcgaa gttggtcatc ctttcgatga aggactgtgc actcgcaccc ttgtccacga
    14281 cctcttcgaa gttccaaggc gtgatagtct cctcggattt gcgtgtcatc cacgcaaacc
    14341 gcgagttccc gcgtgcgagt ggacctaatg aattaataga aaaatcaggc aaattagaaa
    14401 agaaataatt ctcaaaagag aacaactgag gtcagaaaat agcattcatc aactgaaact
    14461 aggtatttct tcatatgata ctaacctaca tagtagggaa tgcggaacgt gaggatcttt
    14521 tcaatcttct ctctgttgtc tttcaggaac ggatagaagt cctcttgcct cctcaagata
    14581 gcatgcagct caccgaggtg gatctggtgc ggtatgctgc cattgtcgaa ggtacgctgc
    14641 ttccgaagca aatcttcgcg gttcaattta acgagcagct cctctgtacc gtccatcttt
    14701 tccagaatag gcttgatgaa tttgtagaat tcctcctgac ttgcacctaa taatatcatc
    14761 agaggaaaac tcaattctta tatcttacag aaatgaacaa caaaatacat gtagcagaga
    14821 ggaaaacaat cataagtaac aacttacctc catcaatgta ccctgcgtag ccattcttcg
    14881 attggtcaaa gaaaatctcc ttgtacttct cggggagttg ctggcgaacc aaagccttca
    14941 gaagggtcag atcctggtga tgctcgtcgt acctcttgat catggaggcg ctaagtggcg
    15001 ctttggttat ctccgtgttg acccgcagaa tgtcactgag caagatagcg tctgacagat
    15061 tcttggcagc caagaacagg tcggcatact gatcacctac aaaagtttaa aagactcaat
    15121 tttaactcta cagcttcaaa taaatttccg caaagaaatt gataggttta gacaacaaaa
    15181 gaagagttcc aatttcaaga gcacctattt gggcgagcag attgtccaga tcatcatcgt
    15241 atgtatcctt tgacagctgc agcttcgcgt cctcggcgag gtcaaagttg cttttgaagt
    15301 taggggtgag gcccagagac agtgcaatca gattcccgaa caagccgttc ttcttctcgc
    15361 ctggcaattg agcgatcaga ttctcaagcc gtctggactt cgaaagtctg gcagacagta
    15421 ttgccttggc gtcgacacct gagattgttt gcaaagaaaa atctcaaagc atgtacaatc
    15481 tataaactta tgatggaaaa agaaaagaaa ggtaactaag gaatgttacc tgatgcattg
    15541 atggggttct cctcgaacag ctgattgtag gtttggacga gctgaatgaa cagtttatcc
    15601 acatcggagt tatccgggtt gaggtcccct tcgatcaaga aatggccacg aaactttatc
    15661 atgtgcgcga gagccaggta tatgagccga aggtctgcct tatcggttga gtccaccagc
    15721 ttcttgcgaa ggtggtatat agtcggatac ttttcatgat aggctacttc gtcaacaata
    15781 ttcccaaaga tggggtgacg ctcgtgtttc ttatcttcct ctaccaggaa ggattcctcg
    15841 aggcgatgga agaacgagtc gtcaacctgc aaaatcaagt ttattgatca tcaagtgagt
    15901 ttacatgtag ttaaatgctt tcagtaatat cccaatgaag caggatatgc tttcagtaca
    15961 aaatacataa gcatactata tcataaaaat ccttaccttc gccatctcgt tgctaaagat
    16021 ctcttggaga taacagattc tgttcttcct gcgagtgtac cttctcctcg cagtacgttt
    16081 aagccttgtc gcctccgcgg tttctccaga gtcaaagagc agagctccga tcaagttctt
    16141 cttaatgctg tgcctatctg tgttgcccag cactttaaat ttcttcgaag gtaccttgta
    16201 ttcatcggtg ataacggccc acccaactga gttcgtacca atatcaagtc caatgctgta
    16261 cttcttgtcc attttccagc tgaccttccg cttcttcttc ggtgggctag aagccattgc
    16321 tgtggtgaga gaaacagagc gtgagctcaa atacacaagt acgaaatcag agttatgaga
    16381 gaggatttgg agaaagaaga tcacctgaag atcgtttgcg tcgcgcgaat gagagagacg
    16441 gcagagaaaa cgagaagctt taagaggacg aacaaaaacc ctaatgtcac tccttttata
    16501 tagatgagaa atttagggtt tgggctctaa tggagctgtc tacatgggct ttcatttctt
    16561 tatccagtaa agaaccggtt taacaggttc cctggtttat acgtggtttt agccggttaa
    16621 cttacggaat attattttta tcttaagtta cacaaattac atcattaata gtaaaacaaa
    16681 attttatggt gcaatttaag tttttatgat gagtgaaatt cgaataatat acaaatattt
    16741 tactgtttag gtttccctaa acctactttt agatatgtaa attaaacatt tttttttttt
    16801 tggttagaac caagtcagtg atcatagaac tcaaaaagct ggtgtttcaa ttggtacaag
    16861 catttttatt gcaagatata aaaattcaga aggacatatc cttgatgtat gtacatgaag
    16921 gaaccgattt tgactccaag ctacaaagaa gaagaagaat gatatacaaa gaaaactgca
    16981 tatcctcacc atgaaattga aagctagagg aaggggaata acagatactc tgcgtagaag
    17041 agattccata taaaccgata atatcctgag atagcttcct gcaataatca aatgtttcta
    17101 cactccgtta gatatacact ccattgatgt tccgtgataa cgagtttatg tttgtacaaa
    17161 agtattttac cttggtgtag tttgcttttt ctagtaccca tgtctaccaa agatcaacaa
    17221 caaagatatt agagattaat gaatttggca gtgtcaaatg cagaaactaa agcatatagt
    17281 tatgaagttc ttgtgcatta tctcaaaagt atgtaagaga tataagagta tctcataatg
    17341 ctttacctgg aaaattaagc ctgaagccaa gatcacatgt gcaggaatca tcaagctacc
    17401 tctaaaaacc tgttcattgt taaaggataa gagggttggt cttttcaaag ctgcatactg
    17461 aggaaagtaa caatatcgca acaaaaataa attggtggcg atagtttatc aagcttctac
    17521 atgcagtaat ggtgttcgtg ctttaattaa ccgaaatagt taagacggaa atagatgaat
    17581 tcacctgagg catgtagaaa gctagtgata tggctgaaac ataatttact agcagaagtc
    17641 cagaaccgag gaatgcaatg tttctcactc caagttttgt tgccagggtt gatatttgga
    17701 acctattcaa gatcaatcaa aggcatgatt aactaaacat gcttgaacaa acacataaag
    17761 acaatatatc aaacggagct ggttaaacat tgtctttgac tgtcaaatgg attgagccaa
    17821 agagcggaaa gaaaatggtt atgcgtgcag ttacttactt tcgatctcct tcaacatcag
    17881 gaaggtcctt tgtaatagca atgaccagtg caaacagtgt cacaaaagat gtgatgaacg
    17941 ccacaggtgc actgcaaata gcgaatcaaa agttgagctc caagcttgaa ttcttgcaga
    18001 gaccgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
    18061 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
    18121 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
    18181 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
    18241 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
    18301 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
    18361 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
    18421 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
    18481 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
    18541 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
    18601 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
    18661 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
    18721 agaatgctta atgaattaca acagtactgc gatgagtggc agggggggcg taaacgccgc
    18781 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
    18841 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
    18901 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
    18961 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
    19021 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
    19081 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
    19141 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
    19201 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
    19261 tgaactttac ccagtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
    19321 ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
    19381 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
    19441 acacagccag tcggtctcag cttctgcagg catgcgacgt cgagcttagc ttgagcttgg
    19501 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
    19561 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
    19621 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
    19681 aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatccaaccc ctccgctgct
    19741 atagtgcagt cggcttctga cgttcagtgc agccgtgttc tgaaaacgac atgtcgcaca
    19801 agtcctaagt tacgcgacag gctgccgccc tgccctt
//
