LOCUS       sequence-309721-       20318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient plant transformation vector containing selection markers 
            and a Cas9 expression cassette, but lacking guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20318)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 20318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE651
FEATURES             Location/Qualifiers
     source          1..20318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6805..7500)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(8706..8958)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8971..9996)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8971..9996)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    9998..10053
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(10089..10268)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(11010..11030)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(16763..16783)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        18493..18595
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             18596..19279
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(18662..18681)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             19599..19904
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     19783..19802
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    20025..20049
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctctctgag
     6361 cgactaaatg gagcaaccta ctgtttttga agtttgggtt gccatgtaat cacttgcaat
     6421 aaatatatgt actaatgact gcatcggctt gcgtgtctaa atttgtagct tattgaaaac
     6481 tttttgtggt taaagacgaa tccagttaac taacataagt ttaacaaaac aagacctttt
     6541 ccatatatag accaccgtta ccaacttaca aagaagccaa ataaagtaca acacaacact
     6601 tgcaagttac aaaaagagag gaaaaaaaac atggtaccat ttgacaacat atagaaacac
     6661 acataaacat tacttataac aaaaagattc aaactcggct tttattatct ccccctgatc
     6721 aaattctaaa cactttattc ctcataaact aactccccga cattggagtt attggactat
     6781 gacgatgaca tcagtggggt aagctcactt gtgccccagt ttgctaggga ggtcgcagta
     6841 tctggccaca gccacctcgt gctgctcgac gtaggtttct ttgtcggcct ccttgattct
     6901 ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt
     6961 ggatctgtat gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc
     7021 catgtcgctt ctgccttcca ggccgccgtc agcggggtac agcatctcgg tgttggcctc
     7081 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat
     7141 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     7201 gcccccatct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     7261 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ctgctgccgt acatgaagct
     7321 ggtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
     7381 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt
     7441 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcat
     7501 agtagtgtgc tggccaccac gagtacggtc acggtcatgt tccccaccag tatgttgctg
     7561 tccgtagtac tgagctctgt ctttcagatc ctgagctttg cttcccaact tcatccttgc
     7621 actgtccaac ttgtctgatc cctgtgggtg ctctcccgtt gcgtacctat tcacaatcaa
     7681 taggtatcat accaagcatt ttacctatat agatgatata cacagacaca ttcaagctag
     7741 ctaggtcaaa tttcgtattt agttaaccaa atgttcccta gaatttgcaa tttcttattg
     7801 ttacatttgt tattcgacca aaaaaattga tccaaagcta ctggctcaac acatgcatgc
     7861 acatattgca caaaattatg aagtaagatg ataaatgtgt gcttacttgt aaatccaaga
     7921 gaaaacggtt atagcggcaa tgccaaaccc tccagaggaa agaaaaccgg tgatgaggag
     7981 tgcaactgtg atgagagccg ggacaaggat tgggctgaag ataacgagca gaggtgttgc
     8041 aacagtcaaa gctatgacag ttccaacaag ggtaaggctg gagagaacaa ggagggaacc
     8101 accagctgtg acagcagttg cagctttagc aatctgccta gacttggagt agtcagatcc
     8161 tcgtccggac atctggtact ggtcacgatc tcggcccatc atcgggtact gatctctgcc
     8221 gatgatatcg tgatgggttc ctctagctgt atccgccatt tttttgttct tgtttactag
     8281 agagaatgag attttgaatg agagattgat caaaaagatg tgtatatatt ttggaagagg
     8341 taggagtgtt tgttagatat attaagaaag gagacgagaa gtcacgtgtc aatggagaca
     8401 tcatgcaatt gtaggccacg tgttaatggc gtcaccatgc agccgctgca tgtgttaacg
     8461 agaggagtct attcaaagac acgtttgttt tttgcctagc tcagaatgtg gcgttggcat
     8521 cgaaccgaaa cataaaacgc aaaacgaacc tttgggctcg tcagtatggg tttgggcctt
     8581 caatagattc cttctatacg gcccacatat ccactattgg caacaaaatt agaaaaattt
     8641 aggatttagt tttaaaattt gttccgcgac attctagaac tccccatggg aattcgtaaa
     8701 gcgtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     8761 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     8821 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     8881 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     8941 ccaaatgttt gaacgatctg cttgacaagc ctattccttt gccctcggac gagtgctggg
     9001 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     9061 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     9121 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     9181 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     9241 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     9301 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     9361 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     9421 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     9481 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     9541 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     9601 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     9661 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     9721 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     9781 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     9841 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     9901 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     9961 ctcgacagac gtcgcggtga gttcaggctt tttcattgta attgtaaata gtaattgtaa
    10021 tgttgtttgt tgtttgttgt tgttggtaat tgttgtaaaa atacagtatg agagtgaata
    10081 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
    10141 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
    10201 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
    10261 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
    10321 acgtgactcc cttaattctc cgctctccga gctcgaattc tagtagcgtt gtcaatcaat
    10381 tggcaagtca taaaatgcat taaaaaatat tttcatactc aactacaaat ccatgagtat
    10441 aactataatt ataaagcaat gattagaatc tgacaaggat tctggaaaat tacataaagg
    10501 aaagttcata aatgtctaaa acacaagagg acatacttgt attcagtaac atttgcagct
    10561 tttctaggtc tgaaaatata tttgttgcct agtgaataag cataatggta caactacaag
    10621 tgttttactc ctcatattaa cttcggtcat tagaggccac gatttgacac atttttactc
    10681 aaaacaaaat gtttgcatat ctcttataat ttcaaattca acacacaaca aataagagaa
    10741 aaaacaaata atattaattt gagaatgaac aaaaggacca tatcattcat taactcttct
    10801 ccatccattt ccatttcaca gttcgatagc gaaaaccgaa taaaaaacac agtaaattac
    10861 aagcacaaca aatggtacaa gaaaaacagt tttcccaatg ccataatact caaactcagt
    10921 aggattctgg tgtgtgcgca atgaaactga tgcattgaac ttgacgaacg ttgtcgaaac
    10981 cgatgatacg aacgaaagct ctaagctcag actttacgct tcttctttgg gtcagcccta
    11041 gagtcaccac ccaactgcga cagatctatc cttgtctcat agagccctgt aatagattgg
    11101 tgaataaggg tagcgtccaa cacttccttg gttgatgtgt agcgctttct gtcgatagtg
    11161 gtatcgaagt acttaaaggc tgctggggct cccagattgg tcagtgtgaa caggtggatg
    11221 atgttttccg cttgttccct tatcggctta tctctgtgct tgttgtacgc ggaaagcacc
    11281 tttaattgta gaaaaccagt aaaacatgga caattaggag gcatcgatta tctaaccaaa
    11341 gactttaaca acactaatct gccactaata ttcatgagaa gtccttacct tatcgaggtt
    11401 agcgtctgca agaatgaccc gcttggaaaa ttcgctgatc tgctctatga tctcatcaag
    11461 atagtgcttg tgctgctcga caaacaactg cttctgctcg ttgtcctctg gtgacccctt
    11521 cagcttctca tagtgtgagg ccaagtacag gaagttgacg tacttactgg gcaatgccag
    11581 ctcgttgccc ttctgcagtt ctccggcaga tgccagcatg cgttttctgc cattttccaa
    11641 ttcgaaaagc gaatacttgg gcagtttgat aatgaggtct ttcttcacct ctttgtaacc
    11701 tggtttgtat aattttggtt cagttaatta ggaaaattac ataatggctc caaaaaaaaa
    11761 ttatcgacct tttaccaaaa aaaataaaat ttatcgacct tgacaggaaa aagttacaaa
    11821 tcaaaagaaa gaatatcgtc tggcatccaa tattttacct ttagcctcga ggaagtcgat
    11881 cggattcttc tcgaacgagg acctttccat tatggtaatt ccaagcagtt ccttgacgct
    11941 tttcagtttc ttgctcttgc ccttctccac cttggccact accagtacgc tgtatgccac
    12001 ggtagggcta tcaaagcctc cgtatttctt cggatcccaa tccttcttcc tcgcaatcag
    12061 tttgtcggag ttgcgcttag ggagaatgga ctccttggag aaaccaccgg tttgaacctc
    12121 cgttttcttg actatgttga cttggggcat gctgagaacc ttacgcacag tcgcaaaatc
    12181 cctgccctta tcccagacaa tctcacctgc aagaaacagc aaagagagag ttaagatata
    12241 agtgaacaag aaagtcttgg gatcgacata catacaatca attgatcaaa ttcctaggat
    12301 taaacatatg aaataggaaa gacagacctg tttcgccatt tgtctcgatc agaggcctct
    12361 tccggatttc accattcgcc agagtgatct ctgtcttgaa gaagttcatg atattcgagt
    12421 aaaagaagta tttcgcggta gccttgccga tctcttgttc tgacttcgcg atcatcttcc
    12481 taacgtcgta caccttgtaa tccccgtaca caaactcaga ctccagtttc ggatacttct
    12541 tgatgagagc agtgccaaca acggcgttca ggtaggcgtc atgggcatga tggtagttgt
    12601 tgatctcgcg aaccttgtaa aactggaagt ccttccggaa gtcactaacc agtttgctct
    12661 tgagggtgat cacctgcgta accatcaaaa gtcaatatgg attcatcaca aattaatcag
    12721 gaatagaaag atcgggtggc cttgaagttg atcaaataaa aagtttcgaa ctttagtttg
    12781 atatggtaaa ggaaagttgg aaactttacc tttacctccc tgataagttt gtcattctca
    12841 tcatacttcg tattcatacg cgaatcgagt atctgggcca catgtttcgt aatctgacga
    12901 gtttcaacca actggcgctt gatgaagccg gctttgtcga gctcagacag tccgcccctt
    12961 tctgccttcg tgagattgtc gaatttccgt tgggttatga gcttagcatt caaaagctgc
    13021 ctccaatagt tcttcatctt cttcacaacc tcctcgctgg gtacattgtc ggattttccg
    13081 cggttcttgt ctgagcgcgt aaggacctat aacacgggag aaaccaaaaa gcaaactcat
    13141 ttcacgccaa atactacaga tttctcaaaa attaagttat aatccatata tcctgacaag
    13201 aaattgatta aaacacagtt gctttacctt gttgtcaata ctatcgtcct tcaggaaaga
    13261 ttgagggaca atgtgatcca catcatagtc ggagagtctg ttaatgtcga gctcttggtc
    13321 gacgtacata tctctcccat tctggaggta atagaggtac agcttctcgt tctggagttg
    13381 agtattttcg actgggtgct ctttcagaat ctgactccca agctccttga tgccttcctc
    13441 tatccgcttc atgcgctccc gtgagttctt ctgccctttc tgagtcgtct ggttttccct
    13501 cgccatctcg atgacgatgt tctccggctt atggcggccc atgacctgta gcaataatta
    13561 aaacaaaaat accacaaaca gttagaactg aggcaagaag aatgaatgca gagaaaaaaa
    13621 atcttagcta ttcatggaaa tggaagtgcg acgaacacct aaaatgcaag gagcataacc
    13681 aaatgcagaa cttaccttca ccagctcatc cacaaccttc actgtctgca gtatgccctt
    13741 ctttatagct ggcgatccag ccaggttggc tatgtgttcg tgaaggctat cgccctgacc
    13801 tgacacttgg gccttctgga tatcttcttt gaaggtcagt gagtcatcgt gaataagctg
    13861 catgaagttc cgattcgcaa aaccgtcgga tttgagaaag tccagtatag tcttgccgga
    13921 ttgcttgtcg cggatgccgt tgatgagctt cctggagagc cttccccaac ctaaaacata
    13981 gaacaaacat ttgtctcatc acaaatatgc atcaaggaac actcagaagt tagtcatata
    14041 cccaaattct tgaaactaga gaaacaaaaa aagagggatc attgcggaaa ccttttgacc
    14101 tcttacctgt atatctcctc ctcttcaact gcttcatgac cttgtcatcg aacagatgcg
    14161 cataagtctt aaggcgctcc tcgatcattt ctctgtcctc aaacagcgtg agagtcagca
    14221 cgatgtcctc gaggatgtcc tcgttctcct cgttgtcaag gaagtccttg tccttgatga
    14281 ttttcaacag gtcatggtat gtgccaagag aggcattgaa cctatcctca acgccgctga
    14341 tttccacaga atcaaagcat tcgatcttct taaagtagtc ctctttaagc tgtttcacag
    14401 tgaccttccg attggttttg aacaagagat cgacaatggc cttcttctgc tcacctgtta
    14461 taacagtaaa aattacttta gaaacaaaac aaaagtgtgg tcttttagga atgattattg
    14521 atcagttcat agtttaaaat cttgtgtttt tatcgtaaaa gtgtggacca ggatttacct
    14581 gacaggaagg ccggtttgcg catcccctcc gtcacatatt tcaccttcgt cagctcgttg
    14641 tagaccgtga agtattcgta caggagacta tgcttgggca ggactttctc atttgggaga
    14701 ttcttatcga agttggtcat cctttcgatg aaggactgtg cactcgcacc cttgtccacg
    14761 acctcttcga agttccaagg cgtgatagtc tcctcggatt tgcgtgtcat ccacgcaaac
    14821 cgcgagttcc cgcgtgcgag tggacctaat gaattaatag aaaaatcagg caaattagaa
    14881 aagaaataat tctcaaaaga gaacaactga ggtcagaaaa tagcattcat caactgaaac
    14941 taggtatttc ttcatatgat actaacctac atagtaggga atgcggaacg tgaggatctt
    15001 ttcaatcttc tctctgttgt ctttcaggaa cggatagaag tcctcttgcc tcctcaagat
    15061 agcatgcagc tcaccgaggt ggatctggtg cggtatgctg ccattgtcga aggtacgctg
    15121 cttccgaagc aaatcttcgc ggttcaattt aacgagcagc tcctctgtac cgtccatctt
    15181 ttccagaata ggcttgatga atttgtagaa ttcctcctga cttgcaccta ataatatcat
    15241 cagaggaaaa ctcaattctt atatcttaca gaaatgaaca acaaaataca tgtagcagag
    15301 aggaaaacaa tcataagtaa caacttacct ccatcaatgt accctgcgta gccattcttc
    15361 gattggtcaa agaaaatctc cttgtacttc tcggggagtt gctggcgaac caaagccttc
    15421 agaagggtca gatcctggtg atgctcgtcg tacctcttga tcatggaggc gctaagtggc
    15481 gctttggtta tctccgtgtt gacccgcaga atgtcactga gcaagatagc gtctgacaga
    15541 ttcttggcag ccaagaacag gtcggcatac tgatcaccta caaaagttta aaagactcaa
    15601 ttttaactct acagcttcaa ataaatttcc gcaaagaaat tgataggttt agacaacaaa
    15661 agaagagttc caatttcaag agcacctatt tgggcgagca gattgtccag atcatcatcg
    15721 tatgtatcct ttgacagctg cagcttcgcg tcctcggcga ggtcaaagtt gcttttgaag
    15781 ttaggggtga ggcccagaga cagtgcaatc agattcccga acaagccgtt cttcttctcg
    15841 cctggcaatt gagcgatcag attctcaagc cgtctggact tcgaaagtct ggcagacagt
    15901 attgccttgg cgtcgacacc tgagattgtt tgcaaagaaa aatctcaaag catgtacaat
    15961 ctataaactt atgatggaaa aagaaaagaa aggtaactaa ggaatgttac ctgatgcatt
    16021 gatggggttc tcctcgaaca gctgattgta ggtttggacg agctgaatga acagtttatc
    16081 cacatcggag ttatccgggt tgaggtcccc ttcgatcaag aaatggccac gaaactttat
    16141 catgtgcgcg agagccaggt atatgagccg aaggtctgcc ttatcggttg agtccaccag
    16201 cttcttgcga aggtggtata tagtcggata cttttcatga taggctactt cgtcaacaat
    16261 attcccaaag atggggtgac gctcgtgttt cttatcttcc tctaccagga aggattcctc
    16321 gaggcgatgg aagaacgagt cgtcaacctg caaaatcaag tttattgatc atcaagtgag
    16381 tttacatgta gttaaatgct ttcagtaata tcccaatgaa gcaggatatg ctttcagtac
    16441 aaaatacata agcatactat atcataaaaa tccttacctt cgccatctcg ttgctaaaga
    16501 tctcttggag ataacagatt ctgttcttcc tgcgagtgta ccttctcctc gcagtacgtt
    16561 taagccttgt cgcctccgcg gtttctccag agtcaaagag cagagctccg atcaagttct
    16621 tcttaatgct gtgcctatct gtgttgccca gcactttaaa tttcttcgaa ggtaccttgt
    16681 attcatcggt gataacggcc cacccaactg agttcgtacc aatatcaagt ccaatgctgt
    16741 acttcttgtc cattttccag ctgaccttcc gcttcttctt cggtgggcta gaagccattg
    16801 ctgtggtgag agaaacagag cgtgagctca aatacacaag tacgaaatca gagttatgag
    16861 agaggatttg gagaaagaag atcacctgaa gatcgtttgc gtcgcgcgaa tgagagagac
    16921 ggcagagaaa acgagaagct ttaagaggac gaacaaaaac cctaatgtca ctccttttat
    16981 atagatgaga aatttagggt ttgggctcta atggagctgt ctacatgggc tttcatttct
    17041 ttatccagta aagaaccggt ttaacaggtt ccctggttta tacgtggttt tagccggtta
    17101 acttacggaa tattattttt atcttaagtt acacaaatta catcattaat agtaaaacaa
    17161 aattttatgg tgcaatttaa gtttttatga tgagtgaaat tcgaataata tacaaatatt
    17221 ttactgttta ggtttcccta aacctacttt tagatatgta aattaaacat tttttttttt
    17281 ttggttagaa ccaagtcagt gatcatagaa ctcaaaaagc tggtgtttca attggtacaa
    17341 gcatttttat tgcaagatat aaaaattcag aaggacatat ccttgatgta tgtacatgaa
    17401 ggaaccgatt ttgactccaa gctacaaaga agaagaagaa tgatatacaa agaaaactgc
    17461 atatcctcac catgaaattg aaagctagag gaaggggaat aacagatact ctgcgtagaa
    17521 gagattccat ataaaccgat aatatcctga gatagcttcc tgcaataatc aaatgtttct
    17581 acactccgtt agatatacac tccattgatg ttccgtgata acgagtttat gtttgtacaa
    17641 aagtatttta ccttggtgta gtttgctttt tctagtaccc atgtctacca aagatcaaca
    17701 acaaagatat tagagattaa tgaatttggc agtgtcaaat gcagaaacta aagcatatag
    17761 ttatgaagtt cttgtgcatt atctcaaaag tatgtaagag atataagagt atctcataat
    17821 gctttacctg gaaaattaag cctgaagcca agatcacatg tgcaggaatc atcaagctac
    17881 ctctaaaaac ctgttcattg ttaaaggata agagggttgg tcttttcaaa gctgcatact
    17941 gaggaaagta acaatatcgc aacaaaaata aattggtggc gatagtttat caagcttcta
    18001 catgcagtaa tggtgttcgt gctttaatta accgaaatag ttaagacgga aatagatgaa
    18061 ttcacctgag gcatgtagaa agctagtgat atggctgaaa cataatttac tagcagaagt
    18121 ccagaaccga ggaatgcaat gtttctcact ccaagttttg ttgccagggt tgatatttgg
    18181 aacctattca agatcaatca aaggcatgat taactaaaca tgcttgaaca aacacataaa
    18241 gacaatatat caaacggagc tggttaaaca ttgtctttga ctgtcaaatg gattgagcca
    18301 aagagcggaa agaaaatggt tatgcgtgca gttacttact ttcgatctcc ttcaacatca
    18361 ggaaggtcct ttgtaatagc aatgaccagt gcaaacagtg tcacaaaaga tgtgatgaac
    18421 gccacaggtg cactgcaaat agcgaatcaa aagttgagct ccaagcttga attcttgcag
    18481 agaccgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
    18541 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
    18601 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
    18661 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
    18721 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
    18781 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
    18841 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
    18901 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
    18961 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
    19021 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
    19081 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
    19141 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
    19201 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggggggc gtaaacgccg
    19261 cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
    19321 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
    19381 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
    19441 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
    19501 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
    19561 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
    19621 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
    19681 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
    19741 gtgaacttta cccagtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
    19801 tggccagtgt gccggtttcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
    19861 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
    19921 tacacagcca gtcggtctca gcttctgcag gcatgcgacg tcgagcttag cttgagcttg
    19981 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
    20041 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
    20101 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
    20161 caaagtactt taaagtactt taaagtactt taaagtactt tgatccaacc cctccgctgc
    20221 tatagtgcag tcggcttctg acgttcagtg cagccgtgtt ctgaaaacga catgtcgcac
    20281 aagtcctaag ttacgcgaca ggctgccgcc ctgccctt
//
