LOCUS       sequence-309729-       20087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient plant transformation vector containing selection markers 
            and a Cas9 expression cassette, but lacking guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20087)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 20087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE652
FEATURES             Location/Qualifiers
     source          1..20087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6805..7500)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(8706..8958)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8971..9765)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9082..9101)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9692..9711
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    9767..9822
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(9858..10037)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(10779..10799)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(16532..16552)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        18262..18364
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             18365..19048
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(18431..18450)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             19368..19673
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     19552..19571
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    19794..19818
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctctctgag
     6361 cgactaaatg gagcaaccta ctgtttttga agtttgggtt gccatgtaat cacttgcaat
     6421 aaatatatgt actaatgact gcatcggctt gcgtgtctaa atttgtagct tattgaaaac
     6481 tttttgtggt taaagacgaa tccagttaac taacataagt ttaacaaaac aagacctttt
     6541 ccatatatag accaccgtta ccaacttaca aagaagccaa ataaagtaca acacaacact
     6601 tgcaagttac aaaaagagag gaaaaaaaac atggtaccat ttgacaacat atagaaacac
     6661 acataaacat tacttataac aaaaagattc aaactcggct tttattatct ccccctgatc
     6721 aaattctaaa cactttattc ctcataaact aactccccga cattggagtt attggactat
     6781 gacgatgaca tcagtggggt aagctcactt gtgccccagt ttgctaggga ggtcgcagta
     6841 tctggccaca gccacctcgt gctgctcgac gtaggtttct ttgtcggcct ccttgattct
     6901 ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt
     6961 ggatctgtat gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc
     7021 catgtcgctt ctgccttcca ggccgccgtc agcggggtac agcatctcgg tgttggcctc
     7081 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat
     7141 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     7201 gcccccatct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     7261 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ctgctgccgt acatgaagct
     7321 ggtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
     7381 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt
     7441 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcat
     7501 agtagtgtgc tggccaccac gagtacggtc acggtcatgt tccccaccag tatgttgctg
     7561 tccgtagtac tgagctctgt ctttcagatc ctgagctttg cttcccaact tcatccttgc
     7621 actgtccaac ttgtctgatc cctgtgggtg ctctcccgtt gcgtacctat tcacaatcaa
     7681 taggtatcat accaagcatt ttacctatat agatgatata cacagacaca ttcaagctag
     7741 ctaggtcaaa tttcgtattt agttaaccaa atgttcccta gaatttgcaa tttcttattg
     7801 ttacatttgt tattcgacca aaaaaattga tccaaagcta ctggctcaac acatgcatgc
     7861 acatattgca caaaattatg aagtaagatg ataaatgtgt gcttacttgt aaatccaaga
     7921 gaaaacggtt atagcggcaa tgccaaaccc tccagaggaa agaaaaccgg tgatgaggag
     7981 tgcaactgtg atgagagccg ggacaaggat tgggctgaag ataacgagca gaggtgttgc
     8041 aacagtcaaa gctatgacag ttccaacaag ggtaaggctg gagagaacaa ggagggaacc
     8101 accagctgtg acagcagttg cagctttagc aatctgccta gacttggagt agtcagatcc
     8161 tcgtccggac atctggtact ggtcacgatc tcggcccatc atcgggtact gatctctgcc
     8221 gatgatatcg tgatgggttc ctctagctgt atccgccatt tttttgttct tgtttactag
     8281 agagaatgag attttgaatg agagattgat caaaaagatg tgtatatatt ttggaagagg
     8341 taggagtgtt tgttagatat attaagaaag gagacgagaa gtcacgtgtc aatggagaca
     8401 tcatgcaatt gtaggccacg tgttaatggc gtcaccatgc agccgctgca tgtgttaacg
     8461 agaggagtct attcaaagac acgtttgttt tttgcctagc tcagaatgtg gcgttggcat
     8521 cgaaccgaaa cataaaacgc aaaacgaacc tttgggctcg tcagtatggg tttgggcctt
     8581 caatagattc cttctatacg gcccacatat ccactattgg caacaaaatt agaaaaattt
     8641 aggatttagt tttaaaattt gttccgcgac attctagaac tccccatggg aattcgtaaa
     8701 gcgtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     8761 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     8821 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     8881 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     8941 ccaaatgttt gaacgatctg cttgacaagc tcagaagaac tcgtcaagaa ggcgatagaa
     9001 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     9061 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     9121 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     9181 attcggcaag caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc
     9241 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     9301 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     9361 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     9421 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     9481 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     9541 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     9601 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     9661 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     9721 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcattgtaa ttgtaaatag
     9781 taattgtaat gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa tacagtatga
     9841 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     9901 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     9961 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc
    10021 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
    10081 ggggtcataa cgtgactccc ttaattctcc gctctccgag ctcgaattct agtagcgttg
    10141 tcaatcaatt ggcaagtcat aaaatgcatt aaaaaatatt ttcatactca actacaaatc
    10201 catgagtata actataatta taaagcaatg attagaatct gacaaggatt ctggaaaatt
    10261 acataaagga aagttcataa atgtctaaaa cacaagagga catacttgta ttcagtaaca
    10321 tttgcagctt ttctaggtct gaaaatatat ttgttgccta gtgaataagc ataatggtac
    10381 aactacaagt gttttactcc tcatattaac ttcggtcatt agaggccacg atttgacaca
    10441 tttttactca aaacaaaatg tttgcatatc tcttataatt tcaaattcaa cacacaacaa
    10501 ataagagaaa aaacaaataa tattaatttg agaatgaaca aaaggaccat atcattcatt
    10561 aactcttctc catccatttc catttcacag ttcgatagcg aaaaccgaat aaaaaacaca
    10621 gtaaattaca agcacaacaa atggtacaag aaaaacagtt ttcccaatgc cataatactc
    10681 aaactcagta ggattctggt gtgtgcgcaa tgaaactgat gcattgaact tgacgaacgt
    10741 tgtcgaaacc gatgatacga acgaaagctc taagctcaga ctttacgctt cttctttggg
    10801 tcagccctag agtcaccacc caactgcgac agatctatcc ttgtctcata gagccctgta
    10861 atagattggt gaataagggt agcgtccaac acttccttgg ttgatgtgta gcgctttctg
    10921 tcgatagtgg tatcgaagta cttaaaggct gctggggctc ccagattggt cagtgtgaac
    10981 aggtggatga tgttttccgc ttgttccctt atcggcttat ctctgtgctt gttgtacgcg
    11041 gaaagcacct ttaattgtag aaaaccagta aaacatggac aattaggagg catcgattat
    11101 ctaaccaaag actttaacaa cactaatctg ccactaatat tcatgagaag tccttacctt
    11161 atcgaggtta gcgtctgcaa gaatgacccg cttggaaaat tcgctgatct gctctatgat
    11221 ctcatcaaga tagtgcttgt gctgctcgac aaacaactgc ttctgctcgt tgtcctctgg
    11281 tgaccccttc agcttctcat agtgtgaggc caagtacagg aagttgacgt acttactggg
    11341 caatgccagc tcgttgccct tctgcagttc tccggcagat gccagcatgc gttttctgcc
    11401 attttccaat tcgaaaagcg aatacttggg cagtttgata atgaggtctt tcttcacctc
    11461 tttgtaacct ggtttgtata attttggttc agttaattag gaaaattaca taatggctcc
    11521 aaaaaaaaat tatcgacctt ttaccaaaaa aaataaaatt tatcgacctt gacaggaaaa
    11581 agttacaaat caaaagaaag aatatcgtct ggcatccaat attttacctt tagcctcgag
    11641 gaagtcgatc ggattcttct cgaacgagga cctttccatt atggtaattc caagcagttc
    11701 cttgacgctt ttcagtttct tgctcttgcc cttctccacc ttggccacta ccagtacgct
    11761 gtatgccacg gtagggctat caaagcctcc gtatttcttc ggatcccaat ccttcttcct
    11821 cgcaatcagt ttgtcggagt tgcgcttagg gagaatggac tccttggaga aaccaccggt
    11881 ttgaacctcc gttttcttga ctatgttgac ttggggcatg ctgagaacct tacgcacagt
    11941 cgcaaaatcc ctgcccttat cccagacaat ctcacctgca agaaacagca aagagagagt
    12001 taagatataa gtgaacaaga aagtcttggg atcgacatac atacaatcaa ttgatcaaat
    12061 tcctaggatt aaacatatga aataggaaag acagacctgt ttcgccattt gtctcgatca
    12121 gaggcctctt ccggatttca ccattcgcca gagtgatctc tgtcttgaag aagttcatga
    12181 tattcgagta aaagaagtat ttcgcggtag ccttgccgat ctcttgttct gacttcgcga
    12241 tcatcttcct aacgtcgtac accttgtaat ccccgtacac aaactcagac tccagtttcg
    12301 gatacttctt gatgagagca gtgccaacaa cggcgttcag gtaggcgtca tgggcatgat
    12361 ggtagttgtt gatctcgcga accttgtaaa actggaagtc cttccggaag tcactaacca
    12421 gtttgctctt gagggtgatc acctgcgtaa ccatcaaaag tcaatatgga ttcatcacaa
    12481 attaatcagg aatagaaaga tcgggtggcc ttgaagttga tcaaataaaa agtttcgaac
    12541 tttagtttga tatggtaaag gaaagttgga aactttacct ttacctccct gataagtttg
    12601 tcattctcat catacttcgt attcatacgc gaatcgagta tctgggccac atgtttcgta
    12661 atctgacgag tttcaaccaa ctggcgcttg atgaagccgg ctttgtcgag ctcagacagt
    12721 ccgccccttt ctgccttcgt gagattgtcg aatttccgtt gggttatgag cttagcattc
    12781 aaaagctgcc tccaatagtt cttcatcttc ttcacaacct cctcgctggg tacattgtcg
    12841 gattttccgc ggttcttgtc tgagcgcgta aggacctata acacgggaga aaccaaaaag
    12901 caaactcatt tcacgccaaa tactacagat ttctcaaaaa ttaagttata atccatatat
    12961 cctgacaaga aattgattaa aacacagttg ctttaccttg ttgtcaatac tatcgtcctt
    13021 caggaaagat tgagggacaa tgtgatccac atcatagtcg gagagtctgt taatgtcgag
    13081 ctcttggtcg acgtacatat ctctcccatt ctggaggtaa tagaggtaca gcttctcgtt
    13141 ctggagttga gtattttcga ctgggtgctc tttcagaatc tgactcccaa gctccttgat
    13201 gccttcctct atccgcttca tgcgctcccg tgagttcttc tgccctttct gagtcgtctg
    13261 gttttccctc gccatctcga tgacgatgtt ctccggctta tggcggccca tgacctgtag
    13321 caataattaa aacaaaaata ccacaaacag ttagaactga ggcaagaaga atgaatgcag
    13381 agaaaaaaaa tcttagctat tcatggaaat ggaagtgcga cgaacaccta aaatgcaagg
    13441 agcataacca aatgcagaac ttaccttcac cagctcatcc acaaccttca ctgtctgcag
    13501 tatgcccttc tttatagctg gcgatccagc caggttggct atgtgttcgt gaaggctatc
    13561 gccctgacct gacacttggg ccttctggat atcttctttg aaggtcagtg agtcatcgtg
    13621 aataagctgc atgaagttcc gattcgcaaa accgtcggat ttgagaaagt ccagtatagt
    13681 cttgccggat tgcttgtcgc ggatgccgtt gatgagcttc ctggagagcc ttccccaacc
    13741 taaaacatag aacaaacatt tgtctcatca caaatatgca tcaaggaaca ctcagaagtt
    13801 agtcatatac ccaaattctt gaaactagag aaacaaaaaa agagggatca ttgcggaaac
    13861 cttttgacct cttacctgta tatctcctcc tcttcaactg cttcatgacc ttgtcatcga
    13921 acagatgcgc ataagtctta aggcgctcct cgatcatttc tctgtcctca aacagcgtga
    13981 gagtcagcac gatgtcctcg aggatgtcct cgttctcctc gttgtcaagg aagtccttgt
    14041 ccttgatgat tttcaacagg tcatggtatg tgccaagaga ggcattgaac ctatcctcaa
    14101 cgccgctgat ttccacagaa tcaaagcatt cgatcttctt aaagtagtcc tctttaagct
    14161 gtttcacagt gaccttccga ttggttttga acaagagatc gacaatggcc ttcttctgct
    14221 cacctgttat aacagtaaaa attactttag aaacaaaaca aaagtgtggt cttttaggaa
    14281 tgattattga tcagttcata gtttaaaatc ttgtgttttt atcgtaaaag tgtggaccag
    14341 gatttacctg acaggaaggc cggtttgcgc atcccctccg tcacatattt caccttcgtc
    14401 agctcgttgt agaccgtgaa gtattcgtac aggagactat gcttgggcag gactttctca
    14461 tttgggagat tcttatcgaa gttggtcatc ctttcgatga aggactgtgc actcgcaccc
    14521 ttgtccacga cctcttcgaa gttccaaggc gtgatagtct cctcggattt gcgtgtcatc
    14581 cacgcaaacc gcgagttccc gcgtgcgagt ggacctaatg aattaataga aaaatcaggc
    14641 aaattagaaa agaaataatt ctcaaaagag aacaactgag gtcagaaaat agcattcatc
    14701 aactgaaact aggtatttct tcatatgata ctaacctaca tagtagggaa tgcggaacgt
    14761 gaggatcttt tcaatcttct ctctgttgtc tttcaggaac ggatagaagt cctcttgcct
    14821 cctcaagata gcatgcagct caccgaggtg gatctggtgc ggtatgctgc cattgtcgaa
    14881 ggtacgctgc ttccgaagca aatcttcgcg gttcaattta acgagcagct cctctgtacc
    14941 gtccatcttt tccagaatag gcttgatgaa tttgtagaat tcctcctgac ttgcacctaa
    15001 taatatcatc agaggaaaac tcaattctta tatcttacag aaatgaacaa caaaatacat
    15061 gtagcagaga ggaaaacaat cataagtaac aacttacctc catcaatgta ccctgcgtag
    15121 ccattcttcg attggtcaaa gaaaatctcc ttgtacttct cggggagttg ctggcgaacc
    15181 aaagccttca gaagggtcag atcctggtga tgctcgtcgt acctcttgat catggaggcg
    15241 ctaagtggcg ctttggttat ctccgtgttg acccgcagaa tgtcactgag caagatagcg
    15301 tctgacagat tcttggcagc caagaacagg tcggcatact gatcacctac aaaagtttaa
    15361 aagactcaat tttaactcta cagcttcaaa taaatttccg caaagaaatt gataggttta
    15421 gacaacaaaa gaagagttcc aatttcaaga gcacctattt gggcgagcag attgtccaga
    15481 tcatcatcgt atgtatcctt tgacagctgc agcttcgcgt cctcggcgag gtcaaagttg
    15541 cttttgaagt taggggtgag gcccagagac agtgcaatca gattcccgaa caagccgttc
    15601 ttcttctcgc ctggcaattg agcgatcaga ttctcaagcc gtctggactt cgaaagtctg
    15661 gcagacagta ttgccttggc gtcgacacct gagattgttt gcaaagaaaa atctcaaagc
    15721 atgtacaatc tataaactta tgatggaaaa agaaaagaaa ggtaactaag gaatgttacc
    15781 tgatgcattg atggggttct cctcgaacag ctgattgtag gtttggacga gctgaatgaa
    15841 cagtttatcc acatcggagt tatccgggtt gaggtcccct tcgatcaaga aatggccacg
    15901 aaactttatc atgtgcgcga gagccaggta tatgagccga aggtctgcct tatcggttga
    15961 gtccaccagc ttcttgcgaa ggtggtatat agtcggatac ttttcatgat aggctacttc
    16021 gtcaacaata ttcccaaaga tggggtgacg ctcgtgtttc ttatcttcct ctaccaggaa
    16081 ggattcctcg aggcgatgga agaacgagtc gtcaacctgc aaaatcaagt ttattgatca
    16141 tcaagtgagt ttacatgtag ttaaatgctt tcagtaatat cccaatgaag caggatatgc
    16201 tttcagtaca aaatacataa gcatactata tcataaaaat ccttaccttc gccatctcgt
    16261 tgctaaagat ctcttggaga taacagattc tgttcttcct gcgagtgtac cttctcctcg
    16321 cagtacgttt aagccttgtc gcctccgcgg tttctccaga gtcaaagagc agagctccga
    16381 tcaagttctt cttaatgctg tgcctatctg tgttgcccag cactttaaat ttcttcgaag
    16441 gtaccttgta ttcatcggtg ataacggccc acccaactga gttcgtacca atatcaagtc
    16501 caatgctgta cttcttgtcc attttccagc tgaccttccg cttcttcttc ggtgggctag
    16561 aagccattgc tgtggtgaga gaaacagagc gtgagctcaa atacacaagt acgaaatcag
    16621 agttatgaga gaggatttgg agaaagaaga tcacctgaag atcgtttgcg tcgcgcgaat
    16681 gagagagacg gcagagaaaa cgagaagctt taagaggacg aacaaaaacc ctaatgtcac
    16741 tccttttata tagatgagaa atttagggtt tgggctctaa tggagctgtc tacatgggct
    16801 ttcatttctt tatccagtaa agaaccggtt taacaggttc cctggtttat acgtggtttt
    16861 agccggttaa cttacggaat attattttta tcttaagtta cacaaattac atcattaata
    16921 gtaaaacaaa attttatggt gcaatttaag tttttatgat gagtgaaatt cgaataatat
    16981 acaaatattt tactgtttag gtttccctaa acctactttt agatatgtaa attaaacatt
    17041 tttttttttt tggttagaac caagtcagtg atcatagaac tcaaaaagct ggtgtttcaa
    17101 ttggtacaag catttttatt gcaagatata aaaattcaga aggacatatc cttgatgtat
    17161 gtacatgaag gaaccgattt tgactccaag ctacaaagaa gaagaagaat gatatacaaa
    17221 gaaaactgca tatcctcacc atgaaattga aagctagagg aaggggaata acagatactc
    17281 tgcgtagaag agattccata taaaccgata atatcctgag atagcttcct gcaataatca
    17341 aatgtttcta cactccgtta gatatacact ccattgatgt tccgtgataa cgagtttatg
    17401 tttgtacaaa agtattttac cttggtgtag tttgcttttt ctagtaccca tgtctaccaa
    17461 agatcaacaa caaagatatt agagattaat gaatttggca gtgtcaaatg cagaaactaa
    17521 agcatatagt tatgaagttc ttgtgcatta tctcaaaagt atgtaagaga tataagagta
    17581 tctcataatg ctttacctgg aaaattaagc ctgaagccaa gatcacatgt gcaggaatca
    17641 tcaagctacc tctaaaaacc tgttcattgt taaaggataa gagggttggt cttttcaaag
    17701 ctgcatactg aggaaagtaa caatatcgca acaaaaataa attggtggcg atagtttatc
    17761 aagcttctac atgcagtaat ggtgttcgtg ctttaattaa ccgaaatagt taagacggaa
    17821 atagatgaat tcacctgagg catgtagaaa gctagtgata tggctgaaac ataatttact
    17881 agcagaagtc cagaaccgag gaatgcaatg tttctcactc caagttttgt tgccagggtt
    17941 gatatttgga acctattcaa gatcaatcaa aggcatgatt aactaaacat gcttgaacaa
    18001 acacataaag acaatatatc aaacggagct ggttaaacat tgtctttgac tgtcaaatgg
    18061 attgagccaa agagcggaaa gaaaatggtt atgcgtgcag ttacttactt tcgatctcct
    18121 tcaacatcag gaaggtcctt tgtaatagca atgaccagtg caaacagtgt cacaaaagat
    18181 gtgatgaacg ccacaggtgc actgcaaata gcgaatcaaa agttgagctc caagcttgaa
    18241 ttcttgcaga gaccgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
    18301 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
    18361 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
    18421 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
    18481 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
    18541 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
    18601 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
    18661 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
    18721 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
    18781 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
    18841 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
    18901 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
    18961 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggggggcg
    19021 taaacgccgc gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
    19081 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
    19141 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
    19201 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
    19261 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
    19321 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
    19381 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
    19441 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
    19501 aagtctcccg tgaactttac ccagtggtgc atatcgggga tgaaagctgg cgcatgatga
    19561 ccaccgatat ggccagtgtg ccggtttccg ttatcgggga agaagtggct gatctcagcc
    19621 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
    19681 gctcccttat acacagccag tcggtctcag cttctgcagg catgcgacgt cgagcttagc
    19741 ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
    19801 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
    19861 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
    19921 cctcgggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatccaaccc
    19981 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtgttc tgaaaacgac
    20041 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgccctt
//
