LOCUS       sequence-309735-        7055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation plasmid for LetAB with LetB PLL6 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
  TITLE     LetB Structure Reveals a Tunnel for Lipid Transport across the 
            Bacterial Envelope.
  JOURNAL   Cell. 2020 Apr 30;181(3):653-664.e19. doi: 
            10.1016/j.cell.2020.03.030.
  PUBMED    32359438
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.03"; journalName: "Cell"; date: "2020-04-30- 
            30"; volume: "181"; issue: "3"; pages: "653-664.e19"
COMMENT     Sequence Label: pBEL1891
FEATURES             Location/Qualifiers
     source          1..7055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     263..282
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        263..281
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             313..335
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(4275..4293)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4289..4336
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4381..4399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4467..4571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4572..4640,4641..5432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4790..4809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5603..6191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6092..6111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6345..6362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6377..6516
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6442..6461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6602..6624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6618..6809)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
       61 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
      121 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
      181 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc
      241 cgagatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
      301 tctagaaata attttgttta actttaagaa ggagatatac atatggctct taacacacca
      361 caaattacgc cgacaaaaaa gataacagtg agggcaatcg gcgaggaact gccgcgtggt
      421 gattaccaac gttgcccgca atgtgacatg ctgtttagcc tgcccgagat aaattctcat
      481 caaagtgcct attgtccgcg ctgtcaggca aaaattcgtg acgggcgcga ctggtcgcta
      541 acgcgcctgg cggcaatggc cttcaccatg ctgttgctta tgccgtttgc ctggggcgaa
      601 ccgctgttgc atatctggct gttaggcatt cgtattgacg ccaacgttat gcaaggcatc
      661 tggcaaatga ccaaacaggg cgatgcgata acggggtcga tggtcttttt ctgcgttatt
      721 ggtgccccgc tcattctggt gacctccata gcttatttat ggtttggtaa ccgactggga
      781 atgaatttac gtccggtact gctgatgctt gagcgactta aagagtgggt aatgctcgat
      841 atctacctgg tcggcattgg cgttgcttct ataaaggtac aggattatgc ccatatccag
      901 gctggtgtcg gcttgttctc ttttgtggcg ttggtgattt taacgacggt gacgttgtca
      961 catcttaatg tcgaagaact gtgggagcga ttttatccgc agcgccccgc tacgcgtagg
     1021 gacgagaaac ttcgtgtctg cctagggtgc cattttaccg gctatccaga tcagcgtggt
     1081 cgctgcccgc gttgccatat cccgctacgc ctgcgtcgcc gtcatagtct gcaaaaatgc
     1141 tgggcggcgc tgttagcgtc aatcgtttta ttgttacctg ccaacctgtt gcctatttct
     1201 atcatttatc tgaacggagg acggcaggaa gatacaattc tttccggaat tatgtcgctg
     1261 gcaagtagca acattgcggt tgcaggaatc gtgtttatcg ccagtattct ggtcccgttt
     1321 actaaagtga tcgtcatgtt cactttactg ttgagcattc attttaaatg ccagcaaggt
     1381 ttacgcacac gcattctgtt actgcggatg gtgacctgga ttggtcgctg gtcgatgctc
     1441 gacctgtttg tcatatcttt aaccatgtcg ctgattaatc gcgatcagat cctcgctttt
     1501 actatgggac cggctgcgtt ttatttcggc gcagcggtaa ttttgactat tcttgctgtg
     1561 gaatggctgg acagccgctt actttgggat gcacatgagt caggaaacgc ccgcttcgac
     1621 gactgaagcg cagattaaaa ataaacgccg tatctcacct ttctggctgc tgccgttcat
     1681 cgcgctaatg attgccagtt ggctgatttg ggacagttat caggaccggg gtaataccgt
     1741 caccatcgac tttatgtcgg cggatggtat tgttccaggc cgtacgcctg ttcgttatca
     1801 gggcgttgaa gtcggaacag tgcaggacat cagcctcagc gacgatcttc gtaagattga
     1861 agtcaaggtc agcatcaagt ccgatatgaa agatgcgctg cgcgaagaga ctcagttctg
     1921 gctggtgacg ccaaaagcat cgttggcagg tgtctccggg ctggacgccc tcgtcggtgg
     1981 gaactatatc ggcatgatgc ccgggaaagg taaagagcag gatcactttg tcgcactcga
     2041 tacccaaccg aaatatcggc tggacaatgg cgatctgatg atccacctgc aagcccccga
     2101 tctcggttcg ctgaacagcg gttcattggt ctatttccgc aagatcccgg tgggaaaagt
     2161 ctacgactat gccatcaatc ccaacaagca aggcgtggtg attgatgtcc tgatcgagcg
     2221 gcgttttacc gatctggtga aaaaaggtag ccgtttctgg aacgtttccg gcgttgatgc
     2281 caacgtcagt atcagtggcg cgaaggtgaa actggaaagc ttggcggcac tggttaacgg
     2341 tgcgattgcc ttcgattcac cagaagagtc gaaacctgcc gaggcggaag atacctttgg
     2401 tctgtatgaa gatctggccc acagccagcg tggcgtaata ataaaactgg aactgccgag
     2461 tggggccgga ttaaccgccg actcgacgcc gttaatgtat caggggctgg aagtcggaca
     2521 gctgactaaa ctggatttaa atcctggtgg taaagtcaca ggggaaatga ccgttgatcc
     2581 cagcgtcgtt accctgcttc gtgaaaatac ccgcatcgaa ttacgcaacc cgaaattatc
     2641 ccttagcgat gctaatctca gcgccctgct gaccggcaaa accttcgagc tggtgcccgg
     2701 cgatggcgag ccacgcaaag aattcgttgt tgtgccaggc gaaaaagcac tgctgcatga
     2761 acctgatgtt ctgacgctga ccctgaccgc gccggaaagt tacggtattg atgcgggtca
     2821 gccgctcatt cttcacggcg tgcaggtagg ccaggttatc gatcgtaaac tcaccagcaa
     2881 aggcgtcacc tttaccgtcg ccatcgagcc tcagcatcgc gaactggtaa aaggcgatag
     2941 caaatttgtc gtcaacagcc gtgtcgatgt gaaggtgggg ctggatggcg ttgagtttct
     3001 cggtgccagc gcctcagaat ggatcaatgg cgggatccgt attctgccgg gcgataaagg
     3061 tgagatgaaa gccagctatc cactgtatgc caatttggaa aaagcgctgg agaacagcct
     3121 tagcgattta cccaccacaa ccgtgagttt gagtgcagag acgctgccgg atgtgcaggc
     3181 aggatcggta gtgctgtacc gtaaatttga agttggtgaa gtgattaccg tgcgtccgcg
     3241 agctaacgcg tttgacatcg atctgcatat taagccggag tatcgcaacc ttctgaccag
     3301 caatagcgtg ttctgggcag aaggcggggc gaaagttcag ctgaatggta gtggtctgac
     3361 cgtacaggca tccccgctct ccagagctct aaagggagcc attagcttcg acaacctcag
     3421 cggtgccagc gccagtcagc gtaaaggcga taagcgtatt ctgtatgctt ccgaaacagc
     3481 ggcccgtgcg gttggcgggc agattacgct tcacgctttc gatgccggaa aactggcggt
     3541 cgggatgcca attcgctatc tcggtattga catcgggcaa atccagacgc tggatctgat
     3601 taccgcacgc aatgaagtac aggcaaaggc ggtactttac ccggaatatg tccagacctt
     3661 tgctcgcggt ggtacgcgct tctcagtggt cacaccggga gggcatcttg atactatcct
     3721 ccagccgtat atcaacgtcg aaccaggccg gggcaatcct cgccgcgact ttgaattaca
     3781 agaggccacc attactgatt cgcggtacct ggatggctta agcattattg ttgaagcgcc
     3841 ggaagccggt tcgttaggca tcggtacgcc tgtgctgttc cgtggtctgg aagtcggtac
     3901 ggttacagga atgacgctgg ggacattgtc agatcgcgtg atgattgcga tgcgcatcag
     3961 taaacgctat caacacctgg tgcgtaacaa ttccgtcttc tggttggcat cgggttacag
     4021 tctggacttt ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc agtttatccg
     4081 tggcggcatc gccttcgcca cgcctccggg tacgccactg gcaccgaaag cccaggaagg
     4141 caaacacttc ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg gaactgcgct
     4201 tcccaaataa tgcccacctc gagcagatcc ggctgctaac aaagcccgaa aggaagctga
     4261 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     4321 cttgaggggt tttttgctga aaggaggaac tatatccgga taattcttga agacgaaagg
     4381 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4441 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4501 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4561 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4621 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4681 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4741 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4801 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4861 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4921 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4981 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5041 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5101 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5161 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5221 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5281 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5341 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5401 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5461 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5521 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5581 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6241 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6301 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6361 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6421 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     6481 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     6541 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6601 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     6661 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     6721 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     6781 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     6841 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     6901 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     6961 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     7021 ccacagggta gccagcagca tcctgcgatg cagat
//
