LOCUS       sequence-309754-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyCatcher003-mi3 in bacterial cells as virus-like 
            particle platform for plug-and-display vaccination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, 
            Bowden TA, Draper SJ, Townsend AR, Howarth M
  TITLE     Overcoming Symmetry Mismatch in Vaccine Nanoassembly through 
            Spontaneous Amidation.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663.
  PUBMED    32886840
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663."
COMMENT     Sequence Label: SpyCatcher003-mi3
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..351
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDG
                     EATEGDAHT"
ORIGIN
        1 atgggttcaa gtgtaaccac cttatcaggt ttatcaggtg aacaaggtcc gtccggtgat
       61 atgacaactg aagaagatag tgctacccat attaaattct caaaacgtga tgaggacggc
      121 cgtgagttag ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca
      181 tggatttcag atggacatgt gaaggatttc tacctgtatc caggaaaata tacatttgtc
      241 gaaaccgcag caccagacgg ttatgaggta gcaactccaa ttgaatttac agttaatgag
      301 gacggtcagg ttactgtaga tggtgaagca actgaaggtg acgctcatac tgggggctca
      361 gggggatctg gcggctctgg cggttcaatg aaaatggaag agcttttcaa gaaacataag
      421 attgtcgcag tattgcgcgc aaacagcgtg gaggaagcga agaaaaaggc tttagcagtg
      481 ttcttgggag gggtgcatct tatagagatc acgttcacag tacctgatgc ggatactgtt
      541 attaaggaat tgtccttctt aaaggaaatg ggggccatta ttggtgcagg gacagtcact
      601 tccgttgagc aagccagaaa agctgtggaa agcggagctg aatttatagt ctccccgcac
      661 ttggacgagg agatcagtca atttgccaaa gaaaaagggg tattttatat gccaggtgta
      721 atgacaccta ccgagttagt gaaagcaatg aagctgggtc acactatatt aaagttgttt
      781 ccgggggaag ttgtagggcc acaattcgtt aaggctatga aagggccatt cccaaatgtt
      841 aaattcgttc ctactggtgg tgtaaatctt gacaatgtct gtgagtggtt taaagccggt
      901 gtccttgccg taggagtcgg atcggcactg gttaagggaa cgcccgtgga ggtagcggag
      961 aaggctaagg ctttcgtgga aaaaattcgt ggctgtacag aaggttctgg cgagcctgag
     1021 gcttaataa
//
