LOCUS       sequence-309755-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, 
            Bowden TA, Draper SJ, Townsend AR, Howarth M
  TITLE     Overcoming Symmetry Mismatch in Vaccine Nanoassembly through 
            Spontaneous Amidation.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663.
  PUBMED    32886840
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663."
COMMENT     Sequence Label: Spy0469
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggccatc atcatcacca ccatggaagc ggagaaaacc tttacttcca aggctcagga
       61 caggctcaag aatggacacc ccgcagcgta acagaaatta aatcggaatt agtattagtg
      121 gacaatgtgt ttacctatac agtaaagtac ggtgatacat taagcactat tgcggaagcg
      181 atgggaatcg acgttcacgt attgggagat attaatcaca ttgctaatat cgaccttatc
      241 tttccggata ctatcttgac ggcgaactac aaccagcacg gtcaggcgac aaacctgact
      301 gttcaggccc cagccagttc acctgcctca gtaagtcatg tcccgtcttc ggaaccgttg
      361 ccgcaagcgt ctgcgacaag ccagcctaca gtccctatgg cgccgcccgc tactccgtcc
      421 gatgtaccaa ccacaccttt cgcgtcggcc aagcctgatt caagtgtcac cgcatcaagt
      481 gagcttacct cttctactaa cgatgttagc accgagttaa gctcagaatc gcagaagcaa
      541 cccgaggtgc cccaagaagc tgtgccaact cccaaagccg cagaaacgac agaggtggaa
      601 cctaagacag atatttcgga agcccccacg tcagctaatc gtccagtccc aaacgagtca
      661 gcaagcgagg aggtttcttc ggcagcaccg gcgcaggctc cagcagaaaa agaagagact
      721 tcggcaccgg cggcccaaaa ggctgtggct gacacaacct ctgtcgcgac atcgaatggt
      781 ctgagttacg ctccgaacca tgcttacaac cctatgaacg cgggtctgca accacagact
      841 gcggccttca aggaagaagt agcgtccgcc tttgggatta ccagtttctc cggttaccgc
      901 ccaggagacc ccggagacca tggcaaggga ttagccatcg attttatggt accggaaaat
      961 tcggcgctgg gagaccaggt cgcgcagtac gcgattgacc atatggccga acgtggcatc
     1021 tcctacgtca tttggaaaca gcgtttctac gcacccttcg cgtccatcta cggtccggcg
     1081 tatacgtgga atccaatgcc agaccgcggg tcgattacag agaatcatta tgaccacgtc
     1141 catgtctcgt tcaatgcgta ataa
//
