LOCUS       sequence-309756-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, 
            Bowden TA, Draper SJ, Townsend AR, Howarth M
  TITLE     Overcoming Symmetry Mismatch in Vaccine Nanoassembly through 
            Spontaneous Amidation.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663.
  PUBMED    32886840
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663."
COMMENT     Sequence Label: PsaA
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggtcatc accaccatca tcatgggtct ggagaaaatc tgtatttcca aggcagtggg
       61 ggtagtgctt gcgcatccgg gaagaaggat acaacttcag ggcagaaact taaggtcgtg
      121 gctacgaatt caattatcgc tgatatcact aaaaacattg cgggcgacaa aattgacttg
      181 cattcaatcg tacccatcgg gcaggacccc cacgaatacg aaccccttcc agaagacgtg
      241 aaaaagacta gcgaggcaga tttaatcttc tacaatggga tcaatttgga gaccggtggc
      301 aatgcttggt tcaccaaatt ggtagaaaac gcgaaaaaaa cagagaataa ggactatttt
      361 gctgttagtg atggtgtaga cgtaatctat ctggaggggc aaaacgaaaa aggaaaagag
      421 gacccgcatg cctggttgaa tcttgaaaac gggattatct ttgccaaaaa cattgcaaaa
      481 caactttcgg ctaaagatcc gaacaacaag gaattctatg agaaaaatct gaaagagtac
      541 acagacaagt tggataagtt ggacaaagaa tccaaagaca agttcaacaa gatcccggct
      601 gagaagaaat tgatcgtaac atcggaggga gcgttcaaat attttagcaa agcatatggc
      661 gtaccgtccg cctacatttg ggagatcaac actgaggaag agggcacccc ggaacaaatc
      721 aaaacgctgg tcgaaaaact tcgtcagaca aaagttccta gcctgtttgt agaaagctct
      781 gtggatgacc gcccgatgaa gacggtatcg caagacacaa atatccctat ctatgcgcaa
      841 atttttacgg actcgattgc cgagcaaggg aaagagggcg atagttatta ctctatgatg
      901 aaatataatc tggacaaaat cgcagaaggg ttagcaaaat aataa
//
