LOCUS       sequence-309758-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal 
            SpyTag003 fusion in bacterial cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, 
            Bowden TA, Draper SJ, Townsend AR, Howarth M
  TITLE     Overcoming Symmetry Mismatch in Vaccine Nanoassembly through 
            Spontaneous Amidation.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663.
  PUBMED    32886840
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663."
COMMENT     Sequence Label: SpyTag003-OspC
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             58..105
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccgt
       61 ggcgtgcctc atatcgtgat ggtggacgcc tacaagcgtt acaagggtag tggtgaaagt
      121 ggtaataaca gcggcaaaga tggtaatgca agcgcaaata gcgcagatga aagcgttaaa
      181 ggtccgaatc tgaccgaaat cagcaaaaaa atcaccgaaa gcaatgcagt tgttctggcc
      241 gttaaagaag ttgaaaccct gctggcaagc attgatgaac tggcaaccaa agcaattggg
      301 aaaaaaatcg gtaataatgg cctggaagca aaccagagca aaaataccag cctgctgagc
      361 ggtgcatatg ccattagcga tctgattgca gaaaaactga atgtgctgaa aaacgaagaa
      421 ctgaaagaga aaatcgatac cgcaaaacag agcagcaccg aatttaccaa taaactgaaa
      481 agcgaacatg ccgtgctggg tttagataat ctgacagatg ataatgccca gcgtgccatt
      541 ctgaaaaaac atgccaataa agataaaggc gcagccgaac tggaaaaact gtttaaagcc
      601 gttgaaaatc tgagcaaagc agcacaggat accctgaaaa atgcagttaa agaactgacc
      661 agtccgattg ttgcagaaag cccgaaaaaa ccgtaataa
//
