LOCUS       sequence-309759-        6187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: pDsRed Monomer C1-YAP2
FEATURES             Location/Qualifiers
     source          1..6187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1227
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     951..970
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    2915..3036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2952..2971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3006..3025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3043..3498)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3180..3201)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3392..3411
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3525..3629
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3631..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3631..3822
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3654..3674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4023..4817
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4023..4817
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4077..4096)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4687..4706
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5005..5024)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5049..5096
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5425..6013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5914..5933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg
      601 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag
      661 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc
      721 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc
      781 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc
      841 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc
      901 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg
      961 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg
     1021 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac
     1081 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac
     1141 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac
     1201 gccgaggccc gccactccgg ctcccagtcc ggactcagat ctcgatcgac ggtacctaag
     1261 gatcccgggc agcagccgcc gcctcaaccg gccccccagg gccaagggca gccgccttcg
     1321 cagcccccgc aggggcaggg cccgccgtcc ggacccgggc aaccggcacc cgcggcgacc
     1381 caggcggcgc cgcaggcacc ccccgccggg catcagatcg tgcacgtccg cggggactcg
     1441 gagaccgacc tggaggcgct cttcaacgcc gtcatgaacc ccaagacggc caacgtgccc
     1501 cagaccgtgc ccatgaggct ccggaagctg cccgactcct tcttcaagcc gccggagccc
     1561 aaatcccact cccgacaggc cagtactgat gcaggcactg caggagccct gactccacag
     1621 catgttcgag ctcattcctc tccagcttct ctgcagttgg gagctgtttc tcctgggaca
     1681 ctgaccccca ctggagtagt ctctggccca gcagctacac ccacagctca gcatcttcga
     1741 cagtcttctt ttgagatacc tgatgatgta cctctgccag caggttggga gatggcaaag
     1801 acatcttctg gtcagagata cttcttaaat cacatcgatc agacaacaac atggcaggac
     1861 cccaggaagg ccatgctgtc ccagatgaac gtcacagccc ccaccagtcc accagtgcag
     1921 cagaatatga tgaactcggc ttcaggtcct cttcctgatg gatgggaaca agccatgact
     1981 caggatggag aaatttacta tataaaccat aagaacaaga ccacctcttg gctagaccca
     2041 aggcttgacc ctcgttttgc catgaaccag agaatcagtc agagtgctcc agtgaaacag
     2101 ccaccacccc tggctcccca gagcccacag ggaggcgtca tgggtggcag caactccaac
     2161 cagcagcaac agatgcgact gcagcaactg cagatggaga aggagaggct gcggctgaaa
     2221 cagcaagaac tgcttcggca ggagttagcc ctgcgtagcc agttaccaac actggagcag
     2281 gatggtggga ctcaaaatcc agtgtcttct cccgggatgt ctcaggaatt gagaacaatg
     2341 acgaccaata gctcagatcc tttccttaac agtggcacct atcactctcg agatgagagt
     2401 acagacagtg gactaagcat gagcagctac agtgtccctc gaaccccaga tgacttcctg
     2461 aacagtgtgg atgagatgga tacaggtgat actatcaacc aaagcaccct gccctcacag
     2521 cagaaccgtt tcccagacta ccttgaagcc attcctggga caaatgtgga ccttggaaca
     2581 ctggaaggag atggaatgaa catagaagga gaggagctga tgccaagtct gcaggaagct
     2641 ttgagttctg acatccttaa tgacatggag tctgttttgg ctgccaccaa gctagataaa
     2701 gaaagctttc ttacatggtt atagagccct caggcagact gaattctgca gatatccagc
     2761 acagtggcgg ccgctcgagt ctagaggatc caccggatct agataactga tcataatcag
     2821 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2881 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3001 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
     3061 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3121 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3181 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3241 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3301 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3361 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3421 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3481 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3601 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4021 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4081 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4141 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4201 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4261 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4321 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4381 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4441 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4501 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4561 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4621 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4681 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4741 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4801 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4861 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4921 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4981 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5041 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5101 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5161 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5221 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6121 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6181 agttccg
//
