LOCUS       sequence-309761-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from 
            Spike) with N-terminal SpyTag fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, 
            Bowden TA, Draper SJ, Townsend AR, Howarth M
  TITLE     Overcoming Symmetry Mismatch in Vaccine Nanoassembly through 
            Spontaneous Amidation.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663.
  PUBMED    32886840
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663."
COMMENT     Sequence Label: SpyTag-RBD
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..111
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 atgaacaccc agatcctggt gttcgccctg atcgccatca ttcccaccaa cgccgacaag
       61 atcggatctg gcgcccacat cgtgatggtg gacgcctaca agcctaccaa aggcagcggt
      121 ggaagcggcg gatctggaac cggtaatatc accaatctgt gccctttcgg cgaggtgttc
      181 aatgccacca gattcgccag cgtgtacgca tggaaccgca agcggataag caattgcgtg
      241 gccgactaca gcgtgctgta caatagcgcc agcttcagca ccttcaaatg ttatggtgtt
      301 tcgccaacaa agctgaatga cctgtgcttc accaatgtgt acgccgacag cttcgtgatc
      361 agaggcgacg aggtgagaca gatcgcgcca gggcagaccg gcaagatcgc cgactacaat
      421 tacaagctgc ctgacgactt caccggctgc gtgatcgcgt ggaactctaa caatctagat
      481 tcgaaagttg gaggcaatta caattacctg tacagactgt tcagaaagag caatctgaag
      541 cctttcgaga gagacatcag caccgagatc taccaggccg gcagcacacc gtgtaatggc
      601 gtggagggct tcaattgcta cttccctctg cagagctacg gcttccagcc taccaatggc
      661 gtgggctacc agccttacag agtggtggtg ctgagcttcg agctgctgca cgctcccgct
      721 accgtgtgcg gccctaagaa gtag
//
