LOCUS       sequence-309769-        8747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding 
            element promoter driving the expression of mStrawberry-pGK-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, 
            Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, 
            Straussman R
  TITLE     Visual barcodes for clonal-multiplexing of live microscopy-based 
            assays.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.
  PUBMED    35585055
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2725. doi"; pages: " 
            10.1038/s41467-022-30008-0"
COMMENT     Sequence Label: HIF-TRE-mStrawberry reporter
FEATURES             Location/Qualifiers
     source          1..8747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             89..799
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(234..252)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     502..521
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    841..958
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1007..1517
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1436..1454
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1529..1924
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    1960..2548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2013..2033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2431..2442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2620..2853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2931..3052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2968..2987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3022..3041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3092..3227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3248..3266)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3276..3293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3276..3292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3285..3307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3434..3889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3521..3540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3731..3752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3915..4019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4020..4088,4089..4880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4238..4257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5051..5639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5540..5559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5793..5810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5927..5948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5963..5968,5969..5986,5987..5993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6001..6017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6006..6028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6025..6041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6025..6041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6060..6080
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6062..6080
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6108..6334
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6335..6515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6562..6687
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7180..7413
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7598..7642
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8016..8047
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     intron          8121..8693
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     8701..8720
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcgacccat ggtgagcaag ggcgaggaga ataacatggc
      121 catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
      181 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      241 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctaaccc ccaacttcac
      301 ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      421 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      481 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      541 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa
      601 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      661 cgtgcagctg cccggcgcct acatcgtcgg catcaagttg gacatcacct cccacaacga
      721 ggactacacc atcgtggaac tgtacgaacg cgccgagggc cgccactcca ccggcggcat
      781 ggacgagctg tacaagtaag aattctagat cttgagacaa atggcagtat tcatccacaa
      841 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      901 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      961 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggttgc
     1021 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     1081 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     1141 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     1201 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     1261 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     1321 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     1381 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     1441 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     1501 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     1561 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     1621 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     1681 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     1741 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     1801 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     1861 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     1921 gggcctgcag ctgcagtagt aaagacgcgt taagtcgaca atcaacctct ggattacaaa
     1981 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2041 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2101 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2161 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2221 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2281 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2341 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     2401 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2461 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2521 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     2581 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2641 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2701 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2761 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2821 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     2881 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     2941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3061 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3121 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3181 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3241 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3301 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3361 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3421 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3481 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3541 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3601 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3661 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3721 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3781 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3841 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     3901 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3961 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4021 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4141 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4201 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4261 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4321 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4381 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4441 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4501 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4561 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4621 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4681 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4861 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5041 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5101 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5161 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5221 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5281 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5341 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5401 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5461 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5521 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5581 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5641 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5701 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5761 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5821 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5881 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5941 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6001 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6061 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6121 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6181 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6241 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6301 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     6361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6481 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6541 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6601 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     6661 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     6721 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     6781 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     6841 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     6901 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     6961 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7021 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7081 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7141 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7201 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7261 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7321 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7381 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7441 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7501 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7561 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7621 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7681 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7741 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     7801 aaccccgagg gaaattcaaa attttatcga ctcagaacac gccgtgtacg tgcttcacgt
     7861 gtacgtggcg atcgtgtacg tgctgtctca cacagcactc tagactactc gtgtacgtgt
     7921 acgacgtgta cgtgggctac gtgtacgtgc tgtctcacac agcacgaagc ttcgtagcaa
     7981 tcggaccgac gacgccgggc tcgagatcta gactctagag ggtatataat ggaagctcga
     8041 attccagctt ggcattccgg tactgttggt aaaaactagg cttttgcaaa aagctccgga
     8101 tcgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     8161 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     8221 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     8281 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     8341 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     8401 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     8461 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     8521 ctggctggcg tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc
     8581 ctttctcttt atggttacaa tgatatacac tgcttgagat gaggataaaa tactctgagt
     8641 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     8701 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattg
//
