LOCUS       sequence-309774-        8549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is a p38 pathway reporter. It has CMV promoter driving 
            the expression of p38-KTR fused to mStrawberry-pGK-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, 
            Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, 
            Straussman R
  TITLE     Visual barcodes for clonal-multiplexing of live microscopy-based 
            assays.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.
  PUBMED    35585055
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2725. doi"; pages: " 
            10.1038/s41467-022-30008-0"
COMMENT     Sequence Label: p38-KTR-mStrawberry reporter
FEATURES             Location/Qualifiers
     source          1..8549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             816..1526
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(961..979)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1229..1248
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1568..1685
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1734..2244
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2163..2181
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2256..2651
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2687..3275
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2740..2760)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3158..3169)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3347..3580
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3658..3779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3695..3714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3749..3768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3819..3954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3974..3993)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3975..3993)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4003..4020)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4003..4019)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4012..4034)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4161..4616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4248..4267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4458..4479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4642..4746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4747..4815,4816..5607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4965..4984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5778..6366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6267..6286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6520..6537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6654..6675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6690..6695,6696..6713,6714..6720)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6728..6744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6733..6755
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6752..6768
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6752..6768
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6787..6807
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6789..6807
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6835..7061
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7062..7242
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7289..7414
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7907..8140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8325..8369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt
      601 ctgcagatat caacaagtgg atgcgtaagc cagatctccg cgttcttatc ccacctggca
      661 gcaagaacac gatgccatca gtgaatcaaa ggataaataa ctccaagcgc tcggccggtc
      721 tcaacgaaga cgagtcgcca tctaagaagc tggcgtcgcc ggaggtgtcg tcaaggctgg
      781 agcgcctgac cctccagtcc agcactagta gacccatggt gagcaagggc gaggagaata
      841 acatggccat catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
      901 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      961 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctaaccccca
     1021 acttcaccta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1081 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1141 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1201 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1261 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga
     1321 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1381 agaagcccgt gcagctgccc ggcgcctaca tcgtcggcat caagttggac atcacctccc
     1441 acaacgagga ctacaccatc gtggaactgt acgaacgcgc cgagggccgc cactccaccg
     1501 gcggcatgga cgagctgtac aagtaagaat tctagatctt gagacaaatg gcagtattca
     1561 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1621 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1681 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc
     1741 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     1801 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     1861 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     1921 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     1981 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     2041 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     2101 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     2161 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     2221 cgaatcaccg acctctctcc ccagggggta ccaccatggc caagcctttg tctcaagaag
     2281 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     2341 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     2401 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     2461 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     2521 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     2581 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     2641 tgtgggaggg cctgcagctg cagtagtaaa gacgcgttaa gtcgacaatc aacctctgga
     2701 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2761 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2821 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2881 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2941 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3001 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3061 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     3121 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3181 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3241 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     3301 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     3361 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     3421 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3481 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3541 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     3601 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     3661 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3721 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3781 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     3961 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4021 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4081 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4141 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4201 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4261 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4321 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4381 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4441 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4501 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4561 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4741 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4801 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4861 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4921 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4981 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5041 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5101 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5161 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5221 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5281 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5341 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5401 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5461 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5521 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5581 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6541 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6601 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6661 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6781 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6841 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     6901 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     6961 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     7021 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     7081 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7141 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7201 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7261 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7321 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7381 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7441 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7501 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7561 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7621 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7681 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7741 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     7981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     8521 acctcccaac cccgaggggc ggccgcccg
//
