LOCUS       sequence-309776-        8570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is a PKA pathway reporter. It has CMV promoter driving 
            the expression of PKA-KTR fused to mStrawberry-pGK-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, 
            Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, 
            Straussman R
  TITLE     Visual barcodes for clonal-multiplexing of live microscopy-based 
            assays.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.
  PUBMED    35585055
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2725. doi"; pages: " 
            10.1038/s41467-022-30008-0"
COMMENT     Sequence Label: PKA-KTR-mStrawberry reporter
FEATURES             Location/Qualifiers
     source          1..8570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             837..1547
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(982..1000)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1250..1269
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1589..1706
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1755..2265
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2184..2202
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2277..2672
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2708..3296
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2761..2781)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3179..3190)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3368..3601
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3679..3800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3716..3735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3770..3789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3840..3975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3995..4014)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3996..4014)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4024..4041)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4024..4040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4033..4055)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4182..4637
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4269..4288)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4479..4500
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4663..4767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4768..4836,4837..5628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4986..5005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5799..6387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6288..6307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6541..6558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6675..6696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6711..6716,6717..6734,6735..6741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6749..6765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6754..6776
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6773..6789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6773..6789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6808..6828
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6810..6828
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6856..7082
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7083..7263
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7310..7435
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7928..8161
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8346..8390
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt
      601 ctgcagatat caacaagtgg atggagatgc cagaggaacc cgccaacagt gggcattcac
      661 tacccccggt ttatatttac agtcccgagt atgtcagcat ctgcgactcc cttgtgaagg
      721 tccccaaacg ggccagtatg gtcaacgaag acgaggcgcc atctagaagg aaggcgtcgg
      781 gtcaggtgtc gtcaaggctg gagcgcctga ccctccagtc cagcactagt agacccatgg
      841 tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      901 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      961 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     1021 cctgggacat cctaaccccc aacttcacct acggctccaa ggcctacgtg aagcaccccg
     1081 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     1141 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     1201 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1261 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     1321 tgaagggcga gatcaagatg aggctgaagc tgaaggacgg cggccactac gacgctgagg
     1381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcggca
     1441 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaactg tacgaacgcg
     1501 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagaa ttctagatct
     1561 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     1621 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     1681 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     1741 ggcgccggct cgagggggcc cgggttgcgc cttttccaag gcagccctgg gtttgcgcag
     1801 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     1861 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     1921 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     1981 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     2041 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     2101 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     2161 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     2221 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagggggt accaccatgg
     2281 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     2341 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     2401 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     2461 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     2521 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     2581 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     2641 aattgctgcc ctctggttat gtgtgggagg gcctgcagct gcagtagtaa agacgcgtta
     2701 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2761 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2821 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2881 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2941 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3001 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3061 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3121 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3181 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3241 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     3301 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     3361 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     3421 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3481 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3541 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3601 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3661 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3721 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3781 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     3841 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3901 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3961 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     4021 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4081 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4141 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     4201 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4261 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4321 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4381 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4441 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4501 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4561 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4621 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4681 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4741 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4801 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4861 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4921 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4981 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5041 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5281 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5941 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6481 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6541 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6601 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6661 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6721 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6781 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     6841 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     6901 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     6961 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     7021 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7081 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7141 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7201 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7261 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7321 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7381 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7441 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7501 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7561 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7621 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7681 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7741 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7801 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7861 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7921 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7981 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8041 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8101 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8161 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8221 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8281 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8341 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8401 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8461 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8521 caccattatc gtttcagacc cacctcccaa ccccgagggg cggccgcccg
//
