LOCUS       sequence-309777-        8686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is a Geminin cell cycle reporter. It has CMV promoter 
            driving the expression of Geminin fused to mStrawberry-pGK-BSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Kaufman T, Nitzan E, Firestein N, Ginzberg MB, Iyengar S, Patel N, 
            Ben-Hamo R, Porat Z, Hunter J, Hilfinger A, Rotter V, Kafri R, 
            Straussman R
  TITLE     Visual barcodes for clonal-multiplexing of live microscopy-based 
            assays.
  JOURNAL   Nat Commun. 2022 May 18;13(1):2725. doi: 10.1038/s41467-022-30008-0.
  PUBMED    35585055
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-18"; volume: "13(1):2725. doi"; pages: " 
            10.1038/s41467-022-30008-0"
COMMENT     Sequence Label: Geminin-mStrawberry reporter
FEATURES             Location/Qualifiers
     source          1..8686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             626..1333
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mStrawberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILTPNFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVELYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(771..789)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1039..1058
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1337..1666
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    1705..1822
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1871..2381
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2300..2318
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2393..2788
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2824..3412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2877..2897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3295..3306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3484..3717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3795..3916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3832..3851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3886..3905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3956..4091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4111..4130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4112..4130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4140..4157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4140..4156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4149..4171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4298..4753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4385..4404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4595..4616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4779..4883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4884..4952,4953..5744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5102..5121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5915..6503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6404..6423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6657..6674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6791..6812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6827..6832,6833..6850,6851..6857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6865..6881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6870..6892
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6889..6905
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6889..6905
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6924..6944
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6926..6944
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6972..7198
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7199..7379
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7426..7551
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8044..8277
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8462..8506
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg tggtggaatt
      601 ctgcagatat caacaagtgg gacccatggt gagcaagggc gaggagaata acatggccat
      661 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt
      721 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      781 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctaaccccca acttcaccta
      841 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      901 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      961 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     1021 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     1081 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga ggctgaagct
     1141 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     1201 gcagctgccc ggcgcctaca tcgtcggcat caagttggac atcacctccc acaacgagga
     1261 ctacaccatc gtggaactgt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     1321 cgagctgtac aaggacatga atcccagtat gaagcagaaa caagaagaaa tcaaagagaa
     1381 tataaagaat agttctgtcc caagaagaac tctgaagatg attcagcctt ctgcatctgg
     1441 atctcttgtt ggaagagaaa atgagctgtc cgcaggcttg tccaaaagga aacatcggaa
     1501 tgaccactta acatctacaa cttccagccc tggggttatt gtcccagaat ctagtgaaaa
     1561 taaaaatctt ggaggagtca cccaggagtc atttgatctt atgattaaag aaaatccatc
     1621 ctctcagtat tggaaggaag tggcagaaaa acggagaaag gcgctgtaat agatcttgag
     1681 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1741 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1801 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     1861 ccggctcgag ggggcccggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     1921 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     1981 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     2041 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     2101 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     2161 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     2221 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     2281 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     2341 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa
     2401 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     2461 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     2521 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     2581 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     2641 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     2701 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     2761 gctgccctct ggttatgtgt gggagggcct gcagctgcag tagtaaagac gcgttaagtc
     2821 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2881 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2941 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3001 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3061 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3121 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3181 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3241 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3301 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3361 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     3421 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3481 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3721 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3781 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3901 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4081 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4141 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4201 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4261 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4741 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4801 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4861 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4921 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4981 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5041 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5101 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5161 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5221 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5281 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5341 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5401 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5461 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5521 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5581 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5641 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5701 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5761 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5821 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6481 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6541 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6601 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6661 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6721 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6781 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6841 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6901 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     6961 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7021 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7081 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7141 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7381 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7441 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7501 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7561 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7621 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7681 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7741 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7801 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     7861 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     7921 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     7981 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8041 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8101 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8161 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8221 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8281 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8341 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8401 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8461 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8521 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8581 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8641 attatcgttt cagacccacc tcccaacccc gaggggcggc cgcccg
//
