LOCUS       sequence-309783-       22139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient plant transformation vector containing selection markers 
            and a Cas9 expression cassette, but lacking guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 22139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE667
FEATURES             Location/Qualifiers
     source          1..22139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6805..7500)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(8706..8958)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8971..9996)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8971..9996)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    9998..10053
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(10089..10268)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(12112..12132)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(17862..18572,18573..18575))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(17904..17925)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     18253..18272
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     18512..18533
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(18588..18614)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        20314..20416
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             20417..21100
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(20483..20502)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             21420..21725
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     21604..21623
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    21846..21870
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctctctgag
     6361 cgactaaatg gagcaaccta ctgtttttga agtttgggtt gccatgtaat cacttgcaat
     6421 aaatatatgt actaatgact gcatcggctt gcgtgtctaa atttgtagct tattgaaaac
     6481 tttttgtggt taaagacgaa tccagttaac taacataagt ttaacaaaac aagacctttt
     6541 ccatatatag accaccgtta ccaacttaca aagaagccaa ataaagtaca acacaacact
     6601 tgcaagttac aaaaagagag gaaaaaaaac atggtaccat ttgacaacat atagaaacac
     6661 acataaacat tacttataac aaaaagattc aaactcggct tttattatct ccccctgatc
     6721 aaattctaaa cactttattc ctcataaact aactccccga cattggagtt attggactat
     6781 gacgatgaca tcagtggggt aagctcactt gtgccccagt ttgctaggga ggtcgcagta
     6841 tctggccaca gccacctcgt gctgctcgac gtaggtttct ttgtcggcct ccttgattct
     6901 ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt
     6961 ggatctgtat gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc
     7021 catgtcgctt ctgccttcca ggccgccgtc agcggggtac agcatctcgg tgttggcctc
     7081 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat
     7141 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     7201 gcccccatct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     7261 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ctgctgccgt acatgaagct
     7321 ggtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
     7381 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt
     7441 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcat
     7501 agtagtgtgc tggccaccac gagtacggtc acggtcatgt tccccaccag tatgttgctg
     7561 tccgtagtac tgagctctgt ctttcagatc ctgagctttg cttcccaact tcatccttgc
     7621 actgtccaac ttgtctgatc cctgtgggtg ctctcccgtt gcgtacctat tcacaatcaa
     7681 taggtatcat accaagcatt ttacctatat agatgatata cacagacaca ttcaagctag
     7741 ctaggtcaaa tttcgtattt agttaaccaa atgttcccta gaatttgcaa tttcttattg
     7801 ttacatttgt tattcgacca aaaaaattga tccaaagcta ctggctcaac acatgcatgc
     7861 acatattgca caaaattatg aagtaagatg ataaatgtgt gcttacttgt aaatccaaga
     7921 gaaaacggtt atagcggcaa tgccaaaccc tccagaggaa agaaaaccgg tgatgaggag
     7981 tgcaactgtg atgagagccg ggacaaggat tgggctgaag ataacgagca gaggtgttgc
     8041 aacagtcaaa gctatgacag ttccaacaag ggtaaggctg gagagaacaa ggagggaacc
     8101 accagctgtg acagcagttg cagctttagc aatctgccta gacttggagt agtcagatcc
     8161 tcgtccggac atctggtact ggtcacgatc tcggcccatc atcgggtact gatctctgcc
     8221 gatgatatcg tgatgggttc ctctagctgt atccgccatt tttttgttct tgtttactag
     8281 agagaatgag attttgaatg agagattgat caaaaagatg tgtatatatt ttggaagagg
     8341 taggagtgtt tgttagatat attaagaaag gagacgagaa gtcacgtgtc aatggagaca
     8401 tcatgcaatt gtaggccacg tgttaatggc gtcaccatgc agccgctgca tgtgttaacg
     8461 agaggagtct attcaaagac acgtttgttt tttgcctagc tcagaatgtg gcgttggcat
     8521 cgaaccgaaa cataaaacgc aaaacgaacc tttgggctcg tcagtatggg tttgggcctt
     8581 caatagattc cttctatacg gcccacatat ccactattgg caacaaaatt agaaaaattt
     8641 aggatttagt tttaaaattt gttccgcgac attctagaac tccccatggg aattcgtaaa
     8701 gcgtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     8761 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     8821 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     8881 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     8941 ccaaatgttt gaacgatctg cttgacaagc ctattccttt gccctcggac gagtgctggg
     9001 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     9061 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     9121 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     9181 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     9241 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     9301 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     9361 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     9421 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     9481 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     9541 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     9601 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     9661 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     9721 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     9781 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     9841 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     9901 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     9961 ctcgacagac gtcgcggtga gttcaggctt tttcattgta attgtaaata gtaattgtaa
    10021 tgttgtttgt tgtttgttgt tgttggtaat tgttgtaaaa atacagtatg agagtgaata
    10081 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
    10141 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
    10201 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
    10261 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
    10321 acgtgactcc cttaattctc cgctctccga gctcgaattc tagtagcgtc ccaaaatctt
    10381 aggaacacgg aatggtacaa aagagtagta cattattgct gaatagctga aacctttgtg
    10441 ttgcataaaa tttcatcatt cgtttgaaac ctttattttt ccccctaatt tgttttgtaa
    10501 atagtgtaat gtttatataa agcttgtaag aatcatgtta aaattatttg taggtttctt
    10561 tgtctctctg caatcgtttg aatcacaaac acctgcggtt atatattgcg tttgcgtttt
    10621 ctgaagtagt agtcatgagt gacttacttg atacacaatt ttaaaatgct attacttgat
    10681 accaaattac tggcgcgttt tatatacggg aatggcaaat tttgtgttgc tataccgata
    10741 actgacgtga cgtggcccgt tattagtcct ggacattcgg tttcagttcg cttccattcg
    10801 gttatcagtt tttctggttc taaagcttag tatccattcg gttattttga aagtttagtt
    10861 cggttctagt ttttttggtt ttaaaatttt gtatccattc ggttgtttag attgaatatt
    10921 tttggatatt ttggttattt ttgaatattt tcggtcattt tggatatttt tcggtcattt
    10981 ttggatattt ttggatattt cggttatttt cgagtatttt tactattata tatatatttt
    11041 taattattta gtctagaaat aactaatatt ttaaaaatat atttatattt cggaaagttt
    11101 cgtttacccg aaatatttcg ctacgtatct atttggtttc agttctttcg attataaaat
    11161 tcagtatcca ttcggttatt tccaactttc ggtacgattc ggtttagtta tttttgtttt
    11221 ggtttcgatt ctgtacagtc ggttcagttt attcgcccag acctacccgt tatatcgagt
    11281 aatatagaaa catttaccaa tttaaagaat caacagagat tagatgatca tatggttagg
    11341 attttaaaaa gagggttttc acaggtcgtg tgaatcacac tctctattca gcatcaaaag
    11401 agttttgaag ttaacgattt acaaacagag aaaagtagac gtgtaggatt tataggaggg
    11461 tacaacagcg ttgtcaatca attggcaagt cataaaatgc attaaaaaat attttcatac
    11521 tcaactacaa atccatgagt ataactataa ttataaagca atgattagaa tctgacaagg
    11581 attctggaaa attacataaa ggaaagttca taaatgtcta aaacacaaga ggacatactt
    11641 gtattcagta acatttgcag cttttctagg tctgaaaata tatttgttgc ctagtgaata
    11701 agcataatgg tacaactaca agtgttttac tcctcatatt aacttcggtc attagaggcc
    11761 acgatttgac acatttttac tcaaaacaaa atgtttgcat atctcttata atttcaaatt
    11821 caacacacaa caaataagag aaaaaacaaa taatattaat ttgagaatga acaaaaggac
    11881 catatcattc attaactctt ctccatccat ttccatttca cagttcgata gcgaaaaccg
    11941 aataaaaaac acagtaaatt acaagcacaa caaatggtac aagaaaaaca gttttcccaa
    12001 tgccataata ctcaaactca gtaggattct ggtgtgtgcg caatgaaact gatgcattga
    12061 acttgacgaa cgttgtcgaa accgatgata cgaacgaaag ctctaagctc agactttacg
    12121 cttcttcttt gggtcagccc tagagtcacc acccaactgc gacagatcta tccttgtctc
    12181 atagagccct gtaatagatt ggtgaataag ggtagcgtcc aacacttcct tggttgatgt
    12241 gtagcgcttt ctgtcgatag tggtatcgaa gtacttaaag gctgctgggg ctcccagatt
    12301 ggtcagtgtg aacaggtgga tgatgttttc cgcttgttcc cttatcggct tatctctgtg
    12361 cttgttgtac gcggaaagca cctttaattg tagaaaacca gtaaaacatg gacaattagg
    12421 aggcatcgat tatctaacca aagactttaa caacactaat ctgccactaa tattcatgag
    12481 aagtccttac cttatcgagg ttagcgtctg caagaatgac ccgcttggaa aattcgctga
    12541 tctgctctat gatctcatca agatagtgct tgtgctgctc gacaaacaac tgcttctgct
    12601 cgttgtcctc tggtgacccc ttcagcttct catagtgtga ggccaagtac aggaagttga
    12661 cgtacttact gggcaatgcc agctcgttgc ccttctgcag ttctccggca gatgccagca
    12721 tgcgttttct gccattttcc aattcgaaaa gcgaatactt gggcagtttg ataatgaggt
    12781 ctttcttcac ctctttgtaa cctggtttgt ataattttgg ttcagttaat taggaaaatt
    12841 acataatggc tccaaaaaaa aattatcgac cttttaccaa aaaaaataaa atttatcgac
    12901 cttgacagga aaaagttaca aatcaaaaga aagaatatcg tctggcatcc aatattttac
    12961 ctttagcctc gaggaagtcg atcggattct tctcgaacga ggacctttcc attatggtaa
    13021 ttccaagcag ttccttgacg cttttcagtt tcttgctctt gcccttctcc accttggcca
    13081 ctaccagtac gctgtatgcc acggtagggc tatcaaagcc tccgtatttc ttcggatccc
    13141 aatccttctt cctcgcaatc agtttgtcgg agttgcgctt agggagaatg gactccttgg
    13201 agaaaccacc ggtttgaacc tccgttttct tgactatgtt gacttggggc atgctgagaa
    13261 ccttacgcac agtcgcaaaa tccctgccct tatcccagac aatctcacct gcaagaaaca
    13321 gcaaagagag agttaagata taagtgaaca agaaagtctt gggatcgaca tacatacaat
    13381 caattgatca aattcctagg attaaacata tgaaatagga aagacagacc tgtttcgcca
    13441 tttgtctcga tcagaggcct cttccggatt tcaccattcg ccagagtgat ctctgtcttg
    13501 aagaagttca tgatattcga gtaaaagaag tatttcgcgg tagccttgcc gatctcttgt
    13561 tctgacttcg cgatcatctt cctaacgtcg tacaccttgt aatccccgta cacaaactca
    13621 gactccagtt tcggatactt cttgatgaga gcagtgccaa caacggcgtt caggtaggcg
    13681 tcatgggcat gatggtagtt gttgatctcg cgaaccttgt aaaactggaa gtccttccgg
    13741 aagtcactaa ccagtttgct cttgagggtg atcacctgcg taaccatcaa aagtcaatat
    13801 ggattcatca caaattaatc aggaatagaa agatcgggtg gccttgaagt tgatcaaata
    13861 aaaagtttcg aactttagtt tgatatggta aaggaaagtt ggaaacttta cctttacctc
    13921 cctgataagt ttgtcattct catcatactt cgtattcata cgcgaatcga gtatctgggc
    13981 cacatgtttc gtaatctgac gagtttcaac caactggcgc ttgatgaagc cggctttgtc
    14041 gagctcagac agtccgcccc tttctgcctt cgtgagattg tcgaatttcc gttgggttat
    14101 gagcttagca ttcaaaagct gcctccaata gttcttcatc ttcttcacaa cctcctcgct
    14161 gggtacattg tcggattttc cgcggttctt gtctgagcgc gtaaggacct ataacacggg
    14221 agaaaccaaa aagcaaactc atttcacgcc aaatactaca gatttctcaa aaattaagtt
    14281 ataatccata tatcctgaca agaaattgat taaaacacag ttgctttacc ttgttgtcaa
    14341 tactatcgtc cttcaggaaa gattgaggga caatgtgatc cacatcatag tcggagagtc
    14401 tgttaatgtc gagctcttgg tcgacgtaca tatctctccc attctggagg taatagaggt
    14461 acagcttctc gttctggagt tgagtatttt cgactgggtg ctctttcaga atctgactcc
    14521 caagctcctt gatgccttcc tctatccgct tcatgcgctc ccgtgagttc ttctgccctt
    14581 tctgagtcgt ctggttttcc ctcgccatct cgatgacgat gttctccggc ttatggcggc
    14641 ccatgacctg tagcaataat taaaacaaaa ataccacaaa cagttagaac tgaggcaaga
    14701 agaatgaatg cagagaaaaa aaatcttagc tattcatgga aatggaagtg cgacgaacac
    14761 ctaaaatgca aggagcataa ccaaatgcag aacttacctt caccagctca tccacaacct
    14821 tcactgtctg cagtatgccc ttctttatag ctggcgatcc agccaggttg gctatgtgtt
    14881 cgtgaaggct atcgccctga cctgacactt gggccttctg gatatcttct ttgaaggtca
    14941 gtgagtcatc gtgaataagc tgcatgaagt tccgattcgc aaaaccgtcg gatttgagaa
    15001 agtccagtat agtcttgccg gattgcttgt cgcggatgcc gttgatgagc ttcctggaga
    15061 gccttcccca acctaaaaca tagaacaaac atttgtctca tcacaaatat gcatcaagga
    15121 acactcagaa gttagtcata tacccaaatt cttgaaacta gagaaacaaa aaaagaggga
    15181 tcattgcgga aaccttttga cctcttacct gtatatctcc tcctcttcaa ctgcttcatg
    15241 accttgtcat cgaacagatg cgcataagtc ttaaggcgct cctcgatcat ttctctgtcc
    15301 tcaaacagcg tgagagtcag cacgatgtcc tcgaggatgt cctcgttctc ctcgttgtca
    15361 aggaagtcct tgtccttgat gattttcaac aggtcatggt atgtgccaag agaggcattg
    15421 aacctatcct caacgccgct gatttccaca gaatcaaagc attcgatctt cttaaagtag
    15481 tcctctttaa gctgtttcac agtgaccttc cgattggttt tgaacaagag atcgacaatg
    15541 gccttcttct gctcacctgt tataacagta aaaattactt tagaaacaaa acaaaagtgt
    15601 ggtcttttag gaatgattat tgatcagttc atagtttaaa atcttgtgtt tttatcgtaa
    15661 aagtgtggac caggatttac ctgacaggaa ggccggtttg cgcatcccct ccgtcacata
    15721 tttcaccttc gtcagctcgt tgtagaccgt gaagtattcg tacaggagac tatgcttggg
    15781 caggactttc tcatttggga gattcttatc gaagttggtc atcctttcga tgaaggactg
    15841 tgcactcgca cccttgtcca cgacctcttc gaagttccaa ggcgtgatag tctcctcgga
    15901 tttgcgtgtc atccacgcaa accgcgagtt cccgcgtgcg agtggaccta atgaattaat
    15961 agaaaaatca ggcaaattag aaaagaaata attctcaaaa gagaacaact gaggtcagaa
    16021 aatagcattc atcaactgaa actaggtatt tcttcatatg atactaacct acatagtagg
    16081 gaatgcggaa cgtgaggatc ttttcaatct tctctctgtt gtctttcagg aacggataga
    16141 agtcctcttg cctcctcaag atagcatgca gctcaccgag gtggatctgg tgcggtatgc
    16201 tgccattgtc gaaggtacgc tgcttccgaa gcaaatcttc gcggttcaat ttaacgagca
    16261 gctcctctgt accgtccatc ttttccagaa taggcttgat gaatttgtag aattcctcct
    16321 gacttgcacc taataatatc atcagaggaa aactcaattc ttatatctta cagaaatgaa
    16381 caacaaaata catgtagcag agaggaaaac aatcataagt aacaacttac ctccatcaat
    16441 gtaccctgcg tagccattct tcgattggtc aaagaaaatc tccttgtact tctcggggag
    16501 ttgctggcga accaaagcct tcagaagggt cagatcctgg tgatgctcgt cgtacctctt
    16561 gatcatggag gcgctaagtg gcgctttggt tatctccgtg ttgacccgca gaatgtcact
    16621 gagcaagata gcgtctgaca gattcttggc agccaagaac aggtcggcat actgatcacc
    16681 tacaaaagtt taaaagactc aattttaact ctacagcttc aaataaattt ccgcaaagaa
    16741 attgataggt ttagacaaca aaagaagagt tccaatttca agagcaccta tttgggcgag
    16801 cagattgtcc agatcatcat cgtatgtatc ctttgacagc tgcagcttcg cgtcctcggc
    16861 gaggtcaaag ttgcttttga agttaggggt gaggcccaga gacagtgcaa tcagattccc
    16921 gaacaagccg ttcttcttct cgcctggcaa ttgagcgatc agattctcaa gccgtctgga
    16981 cttcgaaagt ctggcagaca gtattgcctt ggcgtcgaca cctgagattg tttgcaaaga
    17041 aaaatctcaa agcatgtaca atctataaac ttatgatgga aaaagaaaag aaaggtaact
    17101 aaggaatgtt acctgatgca ttgatggggt tctcctcgaa cagctgattg taggtttgga
    17161 cgagctgaat gaacagttta tccacatcgg agttatccgg gttgaggtcc ccttcgatca
    17221 agaaatggcc acgaaacttt atcatgtgcg cgagagccag gtatatgagc cgaaggtctg
    17281 ccttatcggt tgagtccacc agcttcttgc gaaggtggta tatagtcgga tacttttcat
    17341 gataggctac ttcgtcaaca atattcccaa agatggggtg acgctcgtgt ttcttatctt
    17401 cctctaccag gaaggattcc tcgaggcgat ggaagaacga gtcgtcaacc tgcaaaatca
    17461 agtttattga tcatcaagtg agtttacatg tagttaaatg ctttcagtaa tatcccaatg
    17521 aagcaggata tgctttcagt acaaaataca taagcatact atatcataaa aatccttacc
    17581 ttcgccatct cgttgctaaa gatctcttgg agataacaga ttctgttctt cctgcgagtg
    17641 taccttctcc tcgcagtacg tttaagcctt gtcgcctccg cggtttctcc agagtcaaag
    17701 agcagagctc cgatcaagtt cttcttaatg ctgtgcctat ctgtgttgcc cagcacttta
    17761 aatttcttcg aaggtacctt gtattcatcg gtgataacgg cccacccaac tgagttcgta
    17821 ccaatatcaa gtccaatgct gtacttcttg tccattgcag atttgtacag ctcgtccatg
    17881 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
    17941 ttggggtctt tgctcagctt ggactgagta ctcaggtagt ggttgtcggg cagcagcacg
    18001 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
    18061 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
    18121 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
    18181 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
    18241 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
    18301 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
    18361 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
    18421 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
    18481 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
    18541 accaccccgg tgaacagctc ctcgcccttg ctcactgcgg cagctgagtc gagtttaact
    18601 cttttggcag caggagccat ggtgtggtga gagaaacaga gcgtgagctc aaatacacaa
    18661 gtacgaaatc agagttatga gagaggattt ggagaaagaa gatcacctga agatcgtttg
    18721 cgtcgcgcga atgagagaga cggcagagaa aacgagaagc tttaagagga cgaacaaaaa
    18781 ccctaatgtc actcctttta tatagatgag aaatttaggg tttgggctct aatggagctg
    18841 tctacatggg ctttcatttc tttatccagt aaagaaccgg tttaacaggt tccctggttt
    18901 atacgtggtt ttagccggtt aacttacgga atattatttt tatcttaagt tacacaaatt
    18961 acatcattaa tagtaaaaca aaattttatg gtgcaattta agtttttatg atgagtgaaa
    19021 ttcgaataat atacaaatat tttactgttt aggtttccct aaacctactt ttagatatgt
    19081 aaattaaaca tttttttttt tttggttaga accaagtcag tgatcataga actcaaaaag
    19141 ctggtgtttc aattggtaca agcattttta ttgcaagata taaaaattca gaaggacata
    19201 tccttgatgt atgtacatga aggaaccgat tttgactcca agctacaaag aagaagaaga
    19261 atgatataca aagaaaactg catatcctca ccatgaaatt gaaagctaga ggaaggggaa
    19321 taacagatac tctgcgtaga agagattcca tataaaccga taatatcctg agatagcttc
    19381 ctgcaataat caaatgtttc tacactccgt tagatataca ctccattgat gttccgtgat
    19441 aacgagttta tgtttgtaca aaagtatttt accttggtgt agtttgcttt ttctagtacc
    19501 catgtctacc aaagatcaac aacaaagata ttagagatta atgaatttgg cagtgtcaaa
    19561 tgcagaaact aaagcatata gttatgaagt tcttgtgcat tatctcaaaa gtatgtaaga
    19621 gatataagag tatctcataa tgctttacct ggaaaattaa gcctgaagcc aagatcacat
    19681 gtgcaggaat catcaagcta cctctaaaaa cctgttcatt gttaaaggat aagagggttg
    19741 gtcttttcaa agctgcatac tgaggaaagt aacaatatcg caacaaaaat aaattggtgg
    19801 cgatagttta tcaagcttct acatgcagta atggtgttcg tgctttaatt aaccgaaata
    19861 gttaagacgg aaatagatga attcacctga ggcatgtaga aagctagtga tatggctgaa
    19921 acataattta ctagcagaag tccagaaccg aggaatgcaa tgtttctcac tccaagtttt
    19981 gttgccaggg ttgatatttg gaacctattc aagatcaatc aaaggcatga ttaactaaac
    20041 atgcttgaac aaacacataa agacaatata tcaaacggag ctggttaaac attgtctttg
    20101 actgtcaaat ggattgagcc aaagagcgga aagaaaatgg ttatgcgtgc agttacttac
    20161 tttcgatctc cttcaacatc aggaaggtcc tttgtaatag caatgaccag tgcaaacagt
    20221 gtcacaaaag atgtgatgaa cgccacaggt gcactgcaaa tagcgaatca aaagttgagc
    20281 tccaagcttg aattcttgca gagaccgaga cgttgatcgg cacgtaagag gttccaactt
    20341 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
    20401 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
    20461 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
    20521 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
    20581 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
    20641 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
    20701 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
    20761 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
    20821 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
    20881 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
    20941 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
    21001 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
    21061 gcaggggggg cgtaaacgcc gcgtggatcc ggcttactaa aagccagata acagtatgcg
    21121 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
    21181 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
    21241 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
    21301 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
    21361 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
    21421 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
    21481 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
    21541 tgctgtcaga taaagtctcc cgtgaacttt acccagtggt gcatatcggg gatgaaagct
    21601 ggcgcatgat gaccaccgat atggccagtg tgccggtttc cgttatcggg gaagaagtgg
    21661 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
    21721 tataaatgtc aggctccctt atacacagcc agtcggtctc agcttctgca ggcatgcgac
    21781 gtcgagctta gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
    21841 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
    21901 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
    21961 ccacagggtt cccctcggga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
    22021 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtgt
    22081 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgccctt
//
