LOCUS       sequence-309793-       10021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA5 transfection plasmid for the exogenous expression of 
            N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Gonatopoulos-Pournatzis T, Niibori R, Salter EW, Weatheritt RJ, 
            Tsang B, Farhangmehr S, Liang X, Braunschweig U, Roth J, Zhang S, 
            Henderson T, Sharma E, Quesnel-Vallieres M, Permanyer J, Maier S, 
            Georgiou J, Irimia M, Sonenberg N, Forman-Kay JD, Gingras AC, 
            Collingridge GL, Woodin MA, Cordes SP, Blencowe BJ
  TITLE     Autism-Misregulated eIF4G Microexons Control Synaptic Translation 
            and Higher Order Cognitive Functions.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1176-1192.e16. doi: 
            10.1016/j.molcel.2020.01.006. Epub 2020 Jan 29.
  PUBMED    31999954
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.006"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1176-1192.e16"
COMMENT     Sequence Label: pcDNA5 CMV 3xFG eIF4G1 +MIC
FEATURES             Location/Qualifiers
     source          1..10021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             800..823
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    824..848
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5673..5697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5739..5756)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5745..5969
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6102..6121)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6253..6300
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6308..7330
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7460..7581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7497..7516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7551..7570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7630..7646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7630..7646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7643..7665)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7654..7670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7678..7684,7685..7702,7703..7708))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7723..7744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7861..7878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8032..8620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8112..8131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8791..9582,9583..9651))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9414..9433
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9652..9756)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9831..9850)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttc caccatggac tacaaagacc atgacggtga
      781 tacatatgat catgacatcg attacaagga tgacgatgac aagacaagtt tgtacaaaaa
      841 agcaggcttc aacaaagctc cacagtccac aggcccccca cccgccccat cccccggact
      901 cccacagcca gcgtttcccc cggggcagac agcgccggtg gtgttcagta cgccacaagc
      961 gacacaaatg aacacgcctt ctcagccccg ccagggagga ttcaggtctc tgcagcactt
     1021 ctaccctagc cgggcccagc ccccgagcag tgcagcctcc cgagtgcaga gtgcagcccc
     1081 tgcccgccct ggcccagctg cccatgtcta ccctgctgga tcccaagtaa tgatgatccc
     1141 ttcccagatc tcctacccag cctcccaggg ggcctactac atccctggac aggggcgttc
     1201 cacatacgtt gtcccgacac agcagtaccc tgtgcagcca ggagccccag gcttctatcc
     1261 aggtgcaagc cctacagaat ttgggaccta cgctggcgcc tactatccag cccaaggggt
     1321 gcagcagttt cccactggcg tggcccccgc cccagttttg atgaaccagc caccccagat
     1381 tgctcccaag agggagcgta agacgatccg aattcgagat ccaaaccaag gaggaaagga
     1441 tatcacagag gagatcatgt ctggggcccg cactgcctcc acacccaccc ctccccagac
     1501 gggaggcggt ctggagcctc aagctaatgg ggagacgccc caggttgctg tcattgtccg
     1561 gccagatgac cggtcacagg gagcaatcat tgctgaccgg ccagggctgc ctggcccaga
     1621 gcatagccct tcagaatccc agccttcgtc gccttctccg accccatcac catccccagt
     1681 cttggaaccg gggtctgagc ctaatctcgc agtcctctct attcctgggg acactatgac
     1741 aactatacaa atgtctgtag aagaatcaac ccccatctcc cgtgaaactg gggagccata
     1801 tcgcctctct ccagaaccca ctcctctcgc cgaacccata ctggaagtag aagtgacact
     1861 tagcaaaccg gttccagaat ctgagttttc ttccagtcct ctccaggctc ccaccccttt
     1921 ggcatctcac acagtggaaa ttcatgagcc taatggcatg gtcccatctg aagatctgga
     1981 accagaggtg gagtcaagcc cagagcttgc tcctccccca gcttgcccct ccgaatcccc
     2041 tgtgcccatt gctccaactg cccaacctga ggaactgctc aacggagccc cctcgccacc
     2101 agctgtggac ttaagcccag tcagtgagcc agaggagcag gccaaggagg tgacagcatc
     2161 agtggcgccc cccaccatcc cctctgctac tccagctacg gctccttcag ctacttcccc
     2221 agctcaggag gaggaaatgg aagaagaaga agaagaggaa gaaggagaag caggagaagc
     2281 aggagaagct gagagtgaga aaggaggaga ggaactgctc cccccagaga gtacccctat
     2341 tccagccaac ttgtctcaga atttggaggc agcagcagcc actcaagtgg cagtatctgt
     2401 gccaaagagg agacggaaaa ttaaggagct aaataagaag gaggctgttg gagaccttct
     2461 ggatgccttc aaggaggcga acccggcagt accagaggtg gaaaatcagc ctcctgcagg
     2521 cagcaatcca ggcccagagt ctgagggcag tggtgtgccc ccacgtcctg aggaagcaga
     2581 tgagacctgg gactcaaagg aagacaaaat tcacaatgct gagaacatcc agcccgggga
     2641 acagaagtat gaatataagt cagatcagtg gaagcctcta aacctagagg agaaaaaacg
     2701 ttacgaccgt gagttcctgc ttggttttca gttcatcttt gccagtatgc agaagccaga
     2761 gggattgcca catatcagtg acgtggtgct ggacaaggcc aataaaacac cactgcggcc
     2821 actggatccc actagactac aaggcataaa ttgtggccca gacttcactc catcctttgc
     2881 caaccttggc cggacaaccc ttagcacccg tgggccccca aggggtgggc caggtgggga
     2941 gctgccccgt gggccggctg gcctgggacc ccggcgctct cagcagggac cccgaaaaga
     3001 accacgcaag atcattgcca cagtgttaat gaccgaagat ataaaactga acaaagcaga
     3061 gaaagcctgg aaacccagca gcaagcggac ggcggctgat aaggatcgag gggaagaaga
     3121 tgctgatggc agcaaaaccc aggacctatt ccgcagggtg cgctccatcc tgaataaact
     3181 gacaccccag atgttccagc agctgatgaa gcaagtgacg cagctggcca tcgacaccga
     3241 ggaacgcctc aaaggggtca ttgacctcat ttttgagaag gccatttcag agcccaactt
     3301 ctctgtggcc tatgccaaca tgtgccgctg cctcatggcg ctgaaagtgc ccactacgga
     3361 aaagccaaca gtgactgtga acttccgaaa gctgttgttg aatcgatgtc agaaggagtt
     3421 tgagaaagac aaagatgatg atgaggtttt tgagaagaag caaaaagaga tggatgaagc
     3481 tgctacggca gaggaacgag gacgcctgaa ggaagagctg gaagaggctc gggacatagc
     3541 ccggcggcgc tctttaggga atatcaagtt tattggagag ttgttcaaac tgaagatgtt
     3601 aacagaggca ataatgcatg actgtgtggt caaactgctt aagaaccatg atgaagagtc
     3661 ccttgagtgc ctttgtcgtc tgctcaccac cattggcaaa gacctggact ttgaaaaagc
     3721 caagccccga atggatcagt atttcaacca gatggaaaaa atcattaaag aaaagaagac
     3781 gtcatcccgc atccgcttta tgctgcagga cgtgctggat ctgcgaggga gcaattgggt
     3841 gccacgccga ggggatcagg gtcccaagac cattgaccag atccataagg aggctgagat
     3901 ggaagaacat cgagagcaca tcaaagtgca gcagctcatg gccaagggca gtgacaagcg
     3961 tcggggcggt cctccaggcc ctcccatcag ccgtggactt ccccttgtgg atgatggtgg
     4021 ctggaacaca gttcccatca gcaaaggtag ccgccccatt gacacctcac gactcaccaa
     4081 gatcaccaag cctggctcca tcgattctaa caaccagctc tttgcacctg gagggcgact
     4141 gagctggggc aagggcagca gcggaggctc aggagccaag ccctcagacg cagcatcaga
     4201 agctgctcgc ccagctacta gtactttgaa tcgcttctca gcccttcaac aagcggtacc
     4261 cacagaaagc acagataata gacgtgtggt gcagaggagt agcttgagcc gagaacgagg
     4321 cgagaaagct ggagaccgag gagaccgcct agagcggagt gaacggggag gggaccgtgg
     4381 ggaccggctt gatcgtgcgc ggacacctgc taccaagcgg agcttcagca aggaagtgga
     4441 ggagcggagt agagaacggc cctcccagcc tgaggggctg cgcaaggcag ctagcctcac
     4501 ggaggatcgg gaccgtgggc gggatgccgt gaagcgagaa gctgccctac ccccagtgag
     4561 ccccctgaag gcggctctct ctgaggagga gttagagaag aaatccaagg ctatcattga
     4621 ggaatatctc catctcaatg acatgaaaga ggcagtccag tgcgtgcagg agctggcctc
     4681 accctccttg ctcttcatct ttgtacggca tggtgtcgag tctacgctgg agcgcagtgc
     4741 cattgctcgt gagcatatgg ggcagctgct gcaccagctg ctctgtgctg ggcatctgtc
     4801 tactgctcag tactaccaag ggttgtatga aatcttggaa ttggctgagg acatggaaat
     4861 tgacatcccc cacgtgtggc tctacctagc ggaactggta acacccattc tgcaggaagg
     4921 tggggtgccc atgggggagc tgttcaggga gattacaaag cctctgagac cgttgggcaa
     4981 agctgcttcc ctgttgctgg agatcctggg cctcctgtgc aaaagcatgg gtcctaaaaa
     5041 ggtggggacg ctgtggcgag aagccgggct tagctggaag gaatttctac ctgaaggcca
     5101 ggacattggt gcattcgtcg ctgaacagaa ggtggagtat accctgggag aggagtcgga
     5161 agcccctggc cagagggcac tcccctccga ggagctgaac aggcagctgg agaagctgct
     5221 gaaggagggc agcagtaacc agcgggtgtt cgactggata gaggccaacc tgagtgagca
     5281 gcagatagta tccaacacgt tagttcgagc cctcatgacg gctgtctgct attctgcaat
     5341 tatttttgag actcccctcc gagtggacgt tgcagtgctg aaagcgcgag cgaagctgct
     5401 gcagaaatac ctgtgtgacg agcagaagga gctacaggcg ctctacgccc tccaggccct
     5461 tgtagtgacc ttagaacagc ctcccaacct gctgcggatg ttctttgacg cactgtatga
     5521 cgaggacgtg gtgaaggagg atgccttcta cagttgggag agtagcaagg accccgctga
     5581 gcagcagggc aagggtgtgg cccttaaatc tgtcacagcc ttcttcaagt ggctccgtga
     5641 agcagaggag gagtctgacc acaactgatg agacccagct ttcttgtaca aagtggtctg
     5701 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     5761 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5821 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5881 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5941 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     6001 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6061 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6121 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6181 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6241 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     6301 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     6361 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     6421 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     6481 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     6541 cattggggag ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     6601 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     6661 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     6721 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     6781 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     6841 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     6901 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     6961 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     7021 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     7081 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     7141 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     7201 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     7261 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     7321 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7381 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7441 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7561 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7621 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7681 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7741 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7801 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8461 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8701 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8761 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8881 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8941 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9781 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     9841 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     9901 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     9961 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
    10021 t
//
