LOCUS       sequence-309804-       10689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA5 based transfection plasmid for the exogenous expression of 
            N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for 
            generation of N2A FlpIn cell lines for BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Gonatopoulos-Pournatzis T, Niibori R, Salter EW, Weatheritt RJ, 
            Tsang B, Farhangmehr S, Liang X, Braunschweig U, Roth J, Zhang S, 
            Henderson T, Sharma E, Quesnel-Vallieres M, Permanyer J, Maier S, 
            Georgiou J, Irimia M, Sonenberg N, Forman-Kay JD, Gingras AC, 
            Collingridge GL, Woodin MA, Cordes SP, Blencowe BJ
  TITLE     Autism-Misregulated eIF4G Microexons Control Synaptic Translation 
            and Higher Order Cognitive Functions.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1176-1192.e16. doi: 
            10.1016/j.molcel.2020.01.006. Epub 2020 Jan 29.
  PUBMED    31999954
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.006"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1176-1192.e16"
COMMENT     Sequence Label: pcDNA5 rtTA BirA eIF4G
FEATURES             Location/Qualifiers
     source          1..10689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..351,352..354,355..963)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             994..1017
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1030..1054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5858..5882)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5924..5941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5930..6154
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6287..6306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6438..6485
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6493..7515
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6493..7515
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7645..7766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7682..7701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7736..7755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7815..7831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7815..7831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7828..7850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7839..7855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7863..7869,7870..7887,7888..7893))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7908..7929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8046..8063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8217..8805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8297..8316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8976..9767,9768..9836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9599..9618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9837..9941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10016..10035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    10234..10252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10271..10289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10308..10326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10345..10363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10382..10400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10419..10437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10456..10474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10493..10511
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10533..10557
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgaaggaca acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac
       61 agcggcgagc agctgggcga gaccctgggc atgagccggg ccgccatcaa caagcacatc
      121 cagaccctgc gggactgggg cgtggacgtg ttcaccgtgc ccggcaaggg ctacagcctg
      181 cccgagccca tccagctgct gaacgccaag cagatcctgg gccagctgga cggcggcagc
      241 gtggccgtgc tgcccgtgat cgacagcacc aaccagtacc tgctggaccg gatcggcgag
      301 ctgaagagcg gcgacgcctg catcgccgag taccagcagg ccggccgggg cggccggggc
      361 cggaagtggt tcagcccctt cggcgccaac ctgtacctga gcatgttctg gcggctggag
      421 cagggccccg ccgccgccat cggcctgagc ctggtgatcg gcatcgtgat ggccgaggtg
      481 ctgcggaagc tgggcgccga caaggtgcgg gtgaagtggc ccaacgacct gtacctgcag
      541 gaccggaagc tggccggcat cctggtggag ctgaccggca agaccggcga cgccgcccag
      601 atcgtgatcg gcgccggcat caacatggcc atgcggcggg tggaggagag cgtggtgaac
      661 cagggctgga tcaccctgca ggaggccggc atcaacctgg accggaacac cctggccgcc
      721 atgctgatcc gggagctgcg ggccgccctg gagctgttcg agcaggaggg cctggccccc
      781 tacctgagcc ggtgggagaa gctggacaac ttcatcaacc ggcccgtgaa gctgatcatc
      841 ggcgacaagg agatcttcgg catcagccgg ggcatcgaca agcagggcgc cctgctgctg
      901 gagcaggacg gcatcatcaa gccctggatg ggcggcgaga tcagcctgcg gagcgccgag
      961 aaggggggat ctggccccgg cggcggcgcg ccagactaca aagacgatga cgataaacta
     1021 ggcgcgccca caagtttgta caaaaaagca ggcttcaaca aagctccaca gtccacaggc
     1081 cccccacccg ccccatcccc cggactccca cagccagcgt ttcccccggg gcagacagcg
     1141 ccggtggtgt tcagtacgcc acaagcgaca caaatgaaca cgccttctca gccccgccag
     1201 cacttctacc ctagccgggc ccagcccccg agcagtgcag cctcccgagt gcagagtgca
     1261 gcccctgccc gccctggccc agctgcccat gtctaccctg ctggatccca agtaatgatg
     1321 atcccttccc agatctccta cccagcctcc cagggggcct actacatccc tggacagggg
     1381 cgttccacat acgttgtccc gacacagcag taccctgtgc agccaggagc cccaggcttc
     1441 tatccaggtg caagccctac agaatttggg acctacgctg gcgcctacta tccagcccaa
     1501 ggggtgcagc agtttcccac tggcgtggcc cccgccccag ttttgatgaa ccagccaccc
     1561 cagattgctc ccaagaggga gcgtaagacg atccgaattc gagatccaaa ccaaggagga
     1621 aaggatatca cagaggagat catgtctggg gcccgcactg cctccacacc cacccctccc
     1681 cagacgggag gcggtctgga gcctcaagct aatggggaga cgccccaggt tgctgtcatt
     1741 gtccggccag atgaccggtc acagggagca atcattgctg accggccagg gctgcctggc
     1801 ccagagcata gcccttcaga atcccagcct tcgtcgcctt ctccgacccc atcaccatcc
     1861 ccagtcttgg aaccggggtc tgagcctaat ctcgcagtcc tctctattcc tggggacact
     1921 atgacaacta tacaaatgtc tgtagaagaa tcaaccccca tctcccgtga aactggggag
     1981 ccatatcgcc tctctccaga acccactcct ctcgccgaac ccatactgga agtagaagtg
     2041 acacttagca aaccggttcc agaatctgag ttttcttcca gtcctctcca ggctcccacc
     2101 cctttggcat ctcacacagt ggaaattcat gagcctaatg gcatggtccc atctgaagat
     2161 ctggaaccag aggtggagtc aagcccagag cttgctcctc ccccagcttg cccctccgaa
     2221 tcccctgtgc ccattgctcc aactgcccaa cctgaggaac tgctcaacgg agccccctcg
     2281 ccaccagctg tggacttaag cccagtcagt gagccagagg agcaggccaa ggaggtgaca
     2341 gcatcagtgg cgccccccac catcccctct gctactccag ctacggctcc ttcagctact
     2401 tccccagctc aggaggagga aatggaagaa gaagaagaag aggaagaagg agaagcagga
     2461 gaagcaggag aagctgagag tgagaaagga ggagaggaac tgctcccccc agagagtacc
     2521 cctattccag ccaacttgtc tcagaatttg gaggcagcag cagccactca agtggcagta
     2581 tctgtgccaa agaggagacg gaaaattaag gagctaaata agaaggaggc tgttggagac
     2641 cttctggatg ccttcaagga ggcgaacccg gcagtaccag aggtggaaaa tcagcctcct
     2701 gcaggcagca atccaggccc agagtctgag ggcagtggtg tgcccccacg tcctgaggaa
     2761 gcagatgaga cctgggactc aaaggaagac aaaattcaca atgctgagaa catccagccc
     2821 ggggaacaga agtatgaata taagtcagat cagtggaagc ctctaaacct agaggagaaa
     2881 aaacgttacg accgtgagtt cctgcttggt tttcagttca tctttgccag tatgcagaag
     2941 ccagagggat tgccacatat cagtgacgtg gtgctggaca aggccaataa aacaccactg
     3001 cggccactgg atcccactag actacaaggc ataaattgtg gcccagactt cactccatcc
     3061 tttgccaacc ttggccggac aacccttagc acccgtgggc ccccaagggg tgggccaggt
     3121 ggggagctgc cccgtgggcc ggctggcctg ggaccccggc gctctcagca gggaccccga
     3181 aaagaaccac gcaagatcat tgccacagtg ttaatgaccg aagatataaa actgaacaaa
     3241 gcagagaaag cctggaaacc cagcagcaag cggacggcgg ctgataagga tcgaggggaa
     3301 gaagatgctg atggcagcaa aacccaggac ctattccgca gggtgcgctc catcctgaat
     3361 aaactgacac cccagatgtt ccagcagctg atgaagcaag tgacgcagct ggccatcgac
     3421 accgaggaac gcctcaaagg ggtcattgac ctcatttttg agaaggccat ttcagagccc
     3481 aacttctctg tggcctatgc caacatgtgc cgctgcctca tggcgctgaa agtgcccact
     3541 acggaaaagc caacagtgac tgtgaacttc cgaaagctgt tgttgaatcg atgtcagaag
     3601 gagtttgaga aagacaaaga tgatgatgag gtttttgaga agaagcaaaa agagatggat
     3661 gaagctgcta cggcagagga acgaggacgc ctgaaggaag agctggaaga ggctcgggac
     3721 atagcccggc ggcgctcttt agggaatatc aagtttattg gagagttgtt caaactgaag
     3781 atgttaacag aggcaataat gcatgactgt gtggtcaaac tgcttaagaa ccatgatgaa
     3841 gagtcccttg agtgcctttg tcgtctgctc accaccattg gcaaagacct ggactttgaa
     3901 aaagccaagc cccgaatgga tcagtatttc aaccagatgg aaaaaatcat taaagaaaag
     3961 aagacgtcat cccgcatccg ctttatgctg caggacgtgc tggatctgcg agggagcaat
     4021 tgggtgccac gccgagggga tcagggtccc aagaccattg accagatcca taaggaggct
     4081 gagatggaag aacatcgaga gcacatcaaa gtgcagcagc tcatggccaa gggcagtgac
     4141 aagcgtcggg gcggtcctcc aggccctccc atcagccgtg gacttcccct tgtggatgat
     4201 ggtggctgga acacagttcc catcagcaaa ggtagccgcc ccattgacac ctcacgactc
     4261 accaagatca ccaagcctgg ctccatcgat tctaacaacc agctctttgc acctggaggg
     4321 cgactgagct ggggcaaggg cagcagcgga ggctcaggag ccaagccctc agacgcagca
     4381 tcagaagctg ctcgcccagc tactagtact ttgaatcgct tctcagccct tcaacaagcg
     4441 gtacccacag aaagcacaga taatagacgt gtggtgcaga ggagtagctt gagccgagaa
     4501 cgaggcgaga aagctggaga ccgaggagac cgcctagagc ggagtgaacg gggaggggac
     4561 cgtggggacc ggcttgatcg tgcgcggaca cctgctacca agcggagctt cagcaaggaa
     4621 gtggaggagc ggagtagaga acggccctcc cagcctgagg ggctgcgcaa ggcagctagc
     4681 ctcacggagg atcgggaccg tgggcgggat gccgtgaagc gagaagctgc cctaccccca
     4741 gtgagccccc tgaaggcggc tctctctgag gaggagttag agaagaaatc caaggctatc
     4801 attgaggaat atctccatct caatgacatg aaagaggcag tccagtgcgt gcaggagctg
     4861 gcctcaccct ccttgctctt catctttgta cggcatggtg tcgagtctac gctggagcgc
     4921 agtgccattg ctcgtgagca tatggggcag ctgctgcacc agctgctctg tgctgggcat
     4981 ctgtctactg ctcagtacta ccaagggttg tatgaaatct tggaattggc tgaggacatg
     5041 gaaattgaca tcccccacgt gtggctctac ctagcggaac tggtaacacc cattctgcag
     5101 gaaggtgggg tgcccatggg ggagctgttc agggagatta caaagcctct gagaccgttg
     5161 ggcaaagctg cttccctgtt gctggagatc ctgggcctcc tgtgcaaaag catgggtcct
     5221 aaaaaggtgg ggacgctgtg gcgagaagcc gggcttagct ggaaggaatt tctacctgaa
     5281 ggccaggaca ttggtgcatt cgtcgctgaa cagaaggtgg agtataccct gggagaggag
     5341 tcggaagccc ctggccagag ggcactcccc tccgaggagc tgaacaggca gctggagaag
     5401 ctgctgaagg agggcagcag taaccagcgg gtgttcgact ggatagaggc caacctgagt
     5461 gagcagcaga tagtatccaa cacgttagtt cgagccctca tgacggctgt ctgctattct
     5521 gcaattattt ttgagactcc cctccgagtg gacgttgcag tgctgaaagc gcgagcgaag
     5581 ctgctgcaga aatacctgtg tgacgagcag aaggagctac aggcgctcta cgccctccag
     5641 gcccttgtag tgaccttaga acagcctccc aacctgctgc ggatgttctt tgacgcactg
     5701 tatgacgagg acgtggtgaa ggaggatgcc ttctacagtt gggagagtag caaggacccc
     5761 gctgagcagc agggcaaggg tgtggccctt aaatctgtca cagccttctt caagtggctc
     5821 cgtgaagcag aggaggagtc tgaccacaac tgatgagacc cagctttctt gtacaaagtg
     5881 gtgtgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5941 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6001 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6061 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6121 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6181 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6241 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6301 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6361 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6421 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
     6481 acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     6541 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     6601 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     6661 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     6721 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     6781 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     6841 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     6901 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     6961 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     7021 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     7081 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     7141 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     7201 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     7261 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     7321 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     7381 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     7441 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     7501 agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7561 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7621 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7681 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7741 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7801 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7861 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7921 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7981 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8101 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8641 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8881 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8941 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9001 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9061 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9121 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9181 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9241 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9301 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9361 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9421 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9481 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9541 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9601 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9661 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9721 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9781 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9841 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9901 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9961 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    10021 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
    10081 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
    10141 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
    10201 cgcgtctcga gggatccaag ctggccgcac gtctccctat cagtgataga gaagtcgaca
    10261 cgtctcgagc tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga
    10321 tagagaagtc gacacgtctc gagctcccta tcagtgatag agaaggtacg tctagaacgt
    10381 ctccctatca gtgatagaga agtcgacacg tctcgagctc cctatcagtg atagagaagg
    10441 tacgtctaga acgtctccct atcagtgata gagaagtcga cacgtctcga gctccctatc
    10501 agtgatagag aaggtacccc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
    10561 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
    10621 cgcggaggct ggatcctcta gcgtttaaac ttaagcttgg taccgagctc ggatccagct
    10681 agcgccacc
//
