LOCUS       sequence-30981-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC,
            Brock JE, Richardson AL, Weinberg RA
  TITLE     A pleiotropically acting microRNA, miR-31, inhibits breast cancer 
            metastasis.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1032-46.
  PUBMED    19524507
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1032-46."
COMMENT     Sequence Label: CMV-miR-31 sponge
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     151..172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(547..564)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    553..777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
       61 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      121 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      181 cgggatcact ctcggcatgg acgagctgta caagaagctt agccatggct tcccgccgga
      241 ggtggaggag caggatgatg gcacgctgcc catgtcttgt gcccaggaga gcgggatgga
      301 ccgtcaccct gcagcctgtg cttctgctag gatcaatgtg taggcggccg cctcgagagc
      361 tatgcctcgt cttgcctccg gagctatgcc tcgtcttgcc tccggagcta tgcctcgtct
      421 tgcctccgga gctatgcctc gtcttgcctc cggagctatg cctcgtcttg cctccggagc
      481 tatgcctcgt cttgcctccg gagctatgcc tcgtcttgcc tgggcccgtt taaacccgct
      541 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      601 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      661 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      721 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
      781 ctgangcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
      841 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      901 tagcgcccgc t
//
