LOCUS       sequence-309812-       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA5 based transfection plasmid for the exogenous expression of 
            N-terminal BirA-Flag-tagged eIF4G1 including the microexon for 
            generation of N2A FlpIn cell lines for BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Gonatopoulos-Pournatzis T, Niibori R, Salter EW, Weatheritt RJ, 
            Tsang B, Farhangmehr S, Liang X, Braunschweig U, Roth J, Zhang S, 
            Henderson T, Sharma E, Quesnel-Vallieres M, Permanyer J, Maier S, 
            Georgiou J, Irimia M, Sonenberg N, Forman-Kay JD, Gingras AC, 
            Collingridge GL, Woodin MA, Cordes SP, Blencowe BJ
  TITLE     Autism-Misregulated eIF4G Microexons Control Synaptic Translation 
            and Higher Order Cognitive Functions.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1176-1192.e16. doi: 
            10.1016/j.molcel.2020.01.006. Epub 2020 Jan 29.
  PUBMED    31999954
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.006"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1176-1192.e16"
COMMENT     Sequence Label: pcDNA5 rtTA BirA eIF4G +MIC
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..351,352..354,355..963)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             994..1017
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1030..1054
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5879..5903)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5945..5962)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5951..6175
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6308..6327)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6459..6506
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6514..7536
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6514..7536
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7666..7787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7703..7722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7757..7776
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7836..7852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7836..7852)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7849..7871)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7860..7876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7884..7890,7891..7908,7909..7914))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7929..7950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8067..8084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8238..8826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8318..8337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8997..9788,9789..9857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9620..9639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9858..9962)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10037..10056)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    10255..10273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10292..10310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10329..10347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10366..10384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10403..10421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10440..10458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10477..10495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10514..10532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10554..10578
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgaaggaca acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac
       61 agcggcgagc agctgggcga gaccctgggc atgagccggg ccgccatcaa caagcacatc
      121 cagaccctgc gggactgggg cgtggacgtg ttcaccgtgc ccggcaaggg ctacagcctg
      181 cccgagccca tccagctgct gaacgccaag cagatcctgg gccagctgga cggcggcagc
      241 gtggccgtgc tgcccgtgat cgacagcacc aaccagtacc tgctggaccg gatcggcgag
      301 ctgaagagcg gcgacgcctg catcgccgag taccagcagg ccggccgggg cggccggggc
      361 cggaagtggt tcagcccctt cggcgccaac ctgtacctga gcatgttctg gcggctggag
      421 cagggccccg ccgccgccat cggcctgagc ctggtgatcg gcatcgtgat ggccgaggtg
      481 ctgcggaagc tgggcgccga caaggtgcgg gtgaagtggc ccaacgacct gtacctgcag
      541 gaccggaagc tggccggcat cctggtggag ctgaccggca agaccggcga cgccgcccag
      601 atcgtgatcg gcgccggcat caacatggcc atgcggcggg tggaggagag cgtggtgaac
      661 cagggctgga tcaccctgca ggaggccggc atcaacctgg accggaacac cctggccgcc
      721 atgctgatcc gggagctgcg ggccgccctg gagctgttcg agcaggaggg cctggccccc
      781 tacctgagcc ggtgggagaa gctggacaac ttcatcaacc ggcccgtgaa gctgatcatc
      841 ggcgacaagg agatcttcgg catcagccgg ggcatcgaca agcagggcgc cctgctgctg
      901 gagcaggacg gcatcatcaa gccctggatg ggcggcgaga tcagcctgcg gagcgccgag
      961 aaggggggat ctggccccgg cggcggcgcg ccagactaca aagacgatga cgataaacta
     1021 ggcgcgccca caagtttgta caaaaaagca ggcttcaaca aagctccaca gtccacaggc
     1081 cccccacccg ccccatcccc cggactccca cagccagcgt ttcccccggg gcagacagcg
     1141 ccggtggtgt tcagtacgcc acaagcgaca caaatgaaca cgccttctca gccccgccag
     1201 ggaggattca ggtctctgca gcacttctac cctagccggg cccagccccc gagcagtgca
     1261 gcctcccgag tgcagagtgc agcccctgcc cgccctggcc cagctgccca tgtctaccct
     1321 gctggatccc aagtaatgat gatcccttcc cagatctcct acccagcctc ccagggggcc
     1381 tactacatcc ctggacaggg gcgttccaca tacgttgtcc cgacacagca gtaccctgtg
     1441 cagccaggag ccccaggctt ctatccaggt gcaagcccta cagaatttgg gacctacgct
     1501 ggcgcctact atccagccca aggggtgcag cagtttccca ctggcgtggc ccccgcccca
     1561 gttttgatga accagccacc ccagattgct cccaagaggg agcgtaagac gatccgaatt
     1621 cgagatccaa accaaggagg aaaggatatc acagaggaga tcatgtctgg ggcccgcact
     1681 gcctccacac ccacccctcc ccagacggga ggcggtctgg agcctcaagc taatggggag
     1741 acgccccagg ttgctgtcat tgtccggcca gatgaccggt cacagggagc aatcattgct
     1801 gaccggccag ggctgcctgg cccagagcat agcccttcag aatcccagcc ttcgtcgcct
     1861 tctccgaccc catcaccatc cccagtcttg gaaccggggt ctgagcctaa tctcgcagtc
     1921 ctctctattc ctggggacac tatgacaact atacaaatgt ctgtagaaga atcaaccccc
     1981 atctcccgtg aaactgggga gccatatcgc ctctctccag aacccactcc tctcgccgaa
     2041 cccatactgg aagtagaagt gacacttagc aaaccggttc cagaatctga gttttcttcc
     2101 agtcctctcc aggctcccac ccctttggca tctcacacag tggaaattca tgagcctaat
     2161 ggcatggtcc catctgaaga tctggaacca gaggtggagt caagcccaga gcttgctcct
     2221 cccccagctt gcccctccga atcccctgtg cccattgctc caactgccca acctgaggaa
     2281 ctgctcaacg gagccccctc gccaccagct gtggacttaa gcccagtcag tgagccagag
     2341 gagcaggcca aggaggtgac agcatcagtg gcgcccccca ccatcccctc tgctactcca
     2401 gctacggctc cttcagctac ttccccagct caggaggagg aaatggaaga agaagaagaa
     2461 gaggaagaag gagaagcagg agaagcagga gaagctgaga gtgagaaagg aggagaggaa
     2521 ctgctccccc cagagagtac ccctattcca gccaacttgt ctcagaattt ggaggcagca
     2581 gcagccactc aagtggcagt atctgtgcca aagaggagac ggaaaattaa ggagctaaat
     2641 aagaaggagg ctgttggaga ccttctggat gccttcaagg aggcgaaccc ggcagtacca
     2701 gaggtggaaa atcagcctcc tgcaggcagc aatccaggcc cagagtctga gggcagtggt
     2761 gtgcccccac gtcctgagga agcagatgag acctgggact caaaggaaga caaaattcac
     2821 aatgctgaga acatccagcc cggggaacag aagtatgaat ataagtcaga tcagtggaag
     2881 cctctaaacc tagaggagaa aaaacgttac gaccgtgagt tcctgcttgg ttttcagttc
     2941 atctttgcca gtatgcagaa gccagaggga ttgccacata tcagtgacgt ggtgctggac
     3001 aaggccaata aaacaccact gcggccactg gatcccacta gactacaagg cataaattgt
     3061 ggcccagact tcactccatc ctttgccaac cttggccgga caacccttag cacccgtggg
     3121 cccccaaggg gtgggccagg tggggagctg ccccgtgggc cggctggcct gggaccccgg
     3181 cgctctcagc agggaccccg aaaagaacca cgcaagatca ttgccacagt gttaatgacc
     3241 gaagatataa aactgaacaa agcagagaaa gcctggaaac ccagcagcaa gcggacggcg
     3301 gctgataagg atcgagggga agaagatgct gatggcagca aaacccagga cctattccgc
     3361 agggtgcgct ccatcctgaa taaactgaca ccccagatgt tccagcagct gatgaagcaa
     3421 gtgacgcagc tggccatcga caccgaggaa cgcctcaaag gggtcattga cctcattttt
     3481 gagaaggcca tttcagagcc caacttctct gtggcctatg ccaacatgtg ccgctgcctc
     3541 atggcgctga aagtgcccac tacggaaaag ccaacagtga ctgtgaactt ccgaaagctg
     3601 ttgttgaatc gatgtcagaa ggagtttgag aaagacaaag atgatgatga ggtttttgag
     3661 aagaagcaaa aagagatgga tgaagctgct acggcagagg aacgaggacg cctgaaggaa
     3721 gagctggaag aggctcggga catagcccgg cggcgctctt tagggaatat caagtttatt
     3781 ggagagttgt tcaaactgaa gatgttaaca gaggcaataa tgcatgactg tgtggtcaaa
     3841 ctgcttaaga accatgatga agagtccctt gagtgccttt gtcgtctgct caccaccatt
     3901 ggcaaagacc tggactttga aaaagccaag ccccgaatgg atcagtattt caaccagatg
     3961 gaaaaaatca ttaaagaaaa gaagacgtca tcccgcatcc gctttatgct gcaggacgtg
     4021 ctggatctgc gagggagcaa ttgggtgcca cgccgagggg atcagggtcc caagaccatt
     4081 gaccagatcc ataaggaggc tgagatggaa gaacatcgag agcacatcaa agtgcagcag
     4141 ctcatggcca agggcagtga caagcgtcgg ggcggtcctc caggccctcc catcagccgt
     4201 ggacttcccc ttgtggatga tggtggctgg aacacagttc ccatcagcaa aggtagccgc
     4261 cccattgaca cctcacgact caccaagatc accaagcctg gctccatcga ttctaacaac
     4321 cagctctttg cacctggagg gcgactgagc tggggcaagg gcagcagcgg aggctcagga
     4381 gccaagccct cagacgcagc atcagaagct gctcgcccag ctactagtac tttgaatcgc
     4441 ttctcagccc ttcaacaagc ggtacccaca gaaagcacag ataatagacg tgtggtgcag
     4501 aggagtagct tgagccgaga acgaggcgag aaagctggag accgaggaga ccgcctagag
     4561 cggagtgaac ggggagggga ccgtggggac cggcttgatc gtgcgcggac acctgctacc
     4621 aagcggagct tcagcaagga agtggaggag cggagtagag aacggccctc ccagcctgag
     4681 gggctgcgca aggcagctag cctcacggag gatcgggacc gtgggcggga tgccgtgaag
     4741 cgagaagctg ccctaccccc agtgagcccc ctgaaggcgg ctctctctga ggaggagtta
     4801 gagaagaaat ccaaggctat cattgaggaa tatctccatc tcaatgacat gaaagaggca
     4861 gtccagtgcg tgcaggagct ggcctcaccc tccttgctct tcatctttgt acggcatggt
     4921 gtcgagtcta cgctggagcg cagtgccatt gctcgtgagc atatggggca gctgctgcac
     4981 cagctgctct gtgctgggca tctgtctact gctcagtact accaagggtt gtatgaaatc
     5041 ttggaattgg ctgaggacat ggaaattgac atcccccacg tgtggctcta cctagcggaa
     5101 ctggtaacac ccattctgca ggaaggtggg gtgcccatgg gggagctgtt cagggagatt
     5161 acaaagcctc tgagaccgtt gggcaaagct gcttccctgt tgctggagat cctgggcctc
     5221 ctgtgcaaaa gcatgggtcc taaaaaggtg gggacgctgt ggcgagaagc cgggcttagc
     5281 tggaaggaat ttctacctga aggccaggac attggtgcat tcgtcgctga acagaaggtg
     5341 gagtataccc tgggagagga gtcggaagcc cctggccaga gggcactccc ctccgaggag
     5401 ctgaacaggc agctggagaa gctgctgaag gagggcagca gtaaccagcg ggtgttcgac
     5461 tggatagagg ccaacctgag tgagcagcag atagtatcca acacgttagt tcgagccctc
     5521 atgacggctg tctgctattc tgcaattatt tttgagactc ccctccgagt ggacgttgca
     5581 gtgctgaaag cgcgagcgaa gctgctgcag aaatacctgt gtgacgagca gaaggagcta
     5641 caggcgctct acgccctcca ggcccttgta gtgaccttag aacagcctcc caacctgctg
     5701 cggatgttct ttgacgcact gtatgacgag gacgtggtga aggaggatgc cttctacagt
     5761 tgggagagta gcaaggaccc cgctgagcag cagggcaagg gtgtggccct taaatctgtc
     5821 acagccttct tcaagtggct ccgtgaagca gaggaggagt ctgaccacaa ctgatgagac
     5881 ccagctttct tgtacaaagt ggtgtgactc gagtctagag ggcccgttta aacccgctga
     5941 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6001 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6061 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6121 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6181 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6241 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6301 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6361 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6421 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
     6481 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
     6541 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     6601 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     6661 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     6721 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     6781 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     6841 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     6901 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     6961 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     7021 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     7081 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     7141 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     7201 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     7261 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     7321 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     7381 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     7441 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     7501 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     7561 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7621 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7681 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7741 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7801 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7861 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7921 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7981 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8041 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8641 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9961 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    10021 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    10081 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    10141 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    10201 gcgatgtacg ggccagatat acgcgtctcg agggatccaa gctggccgca cgtctcccta
    10261 tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga aggtacgtct
    10321 agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata
    10381 gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct
    10441 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg
    10501 acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag cagagctcgt
    10561 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
    10621 caccgggacc gatccagcct ccgcggaggc tggatcctct agcgtttaaa cttaagcttg
    10681 gtaccgagct cggatccagc tagcgccacc
//
