LOCUS       sequence-309826-        9962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-EYFP-ccdB-Blast
FEATURES             Location/Qualifiers
     source          1..9962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(543..545,546..548,549..1259)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(588..609)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(849..868)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1196..1217
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1290..1414
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1439..1444,1445..1462,1463..1469)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1523..2182
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1589..1608)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2524..2829
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2708..2727
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2870..2994)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2999..3040
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3054..3071
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3081..3591
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3510..3528
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3607..4002
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4021..4074
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4075..4692,4693..4698,4699..4734,4735..4737,4738..4773,
                     4774..4776,4777..4812,4813..4821)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4861..4881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5279..5290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5495..5728
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5800..5921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5837..5856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5891..5910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5961..6096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6096..6167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6168..6236,6237..7028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6386..6405)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7199..7787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7688..7707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7941..7958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8075..8096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8111..8116,8117..8134,8135..8141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8149..8165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8154..8176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8173..8189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8173..8189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8208..8228
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8210..8228
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8256..8482
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8483..8663
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8710..8835
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9328..9561
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9746..9790
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      601 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      661 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      721 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      781 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      841 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      901 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      961 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1021 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1081 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1141 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1201 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1261 gggccggcca tatcagcggc tctggttcga caagtttgta caaaaaagct gaacgagaaa
     1321 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1381 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1441 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1501 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1561 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1621 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1681 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1741 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1801 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1861 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1921 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1981 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2041 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2101 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2161 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     2221 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2281 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2341 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2401 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2461 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2521 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2581 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2641 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2701 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2761 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2821 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2881 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2941 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgcagg
     3001 aaagccaatc ccaaacccac tcctaggtct cgacagtaca gcatagaccg gtccaccacc
     3061 accaccacca ctaaggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     3121 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     3181 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     3241 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     3301 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     3361 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     3421 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     3481 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     3541 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gcttaattaa
     3601 ttcaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     3661 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     3721 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     3781 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     3841 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     3901 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     3961 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gccctgcagg tcccggtgcc
     4021 gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg tccaatgtct
     4081 agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc
     4141 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     4201 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     4261 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     4321 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     4381 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     4441 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     4501 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     4561 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
     4621 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     4681 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
     4741 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     4801 cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta ttcttaacta
     4861 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4921 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4981 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5041 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5101 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5161 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5221 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5281 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5341 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt
     5401 ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5461 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5521 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5581 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5641 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5701 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5761 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     5821 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5881 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     5941 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6001 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6061 tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat ccgctcatga
     6121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7321 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7861 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7921 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7981 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8041 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8101 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8161 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8221 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     8281 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8341 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8401 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8461 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     8521 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8581 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8641 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8701 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     8761 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8821 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8881 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8941 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9001 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9061 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9121 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     9181 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     9241 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     9301 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     9361 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     9421 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     9481 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     9541 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     9601 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9661 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9721 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9781 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9841 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9901 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9961 ac
//
