LOCUS       sequence-309838-        7005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson 
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under 
            replicating and non-replicating conditions using optimized far-red 
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
  PUBMED    20352111
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(3):e9823."
COMMENT     Sequence Label: pASTA3
FEATURES             Location/Qualifiers
     source          1..7005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1760..1776)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1761..1777)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      5976..6564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6465..6484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6718..6735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6852..6873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6888..6893,6894..6911,6912..6918)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6926..6942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6931..6953
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6950..6966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6950..6966
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctagaggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct ctgggagtac ccgtgtgtac
      241 gaccagcacg gcatacatca tttcgacgcc gagagattcg ccgcccgaaa tgagcacgat
      301 ccggatccag gagggaattc atggtgagta agggcgagga ggtcattaag gagttcatgc
      361 ggttcaaggt caggatggag ggcagcatga acggtcatga gttcgaaatc gaaggagaag
      421 gagagggccg gccgtacgag gggacgcaga ccgcgaagct gaaggtgacg aagggcggcc
      481 cactcccctt cgcatgggac atcctgtcgc cgcagttcat gtatggttcc aaggcgtacg
      541 tgaagcatcc ggcggacata ccggactaca agaagctgag cttcccggag gggttcaagt
      601 gggagcgggt catgaacttt gaggacggcg gccttgtcac agtgacccag gatagcagtt
      661 tgcaggacgg cacgctgatc tataaggtga agatgcgagg gaccaacttc ccgcccgacg
      721 gccccgtgat gcagaagaag accatgggct gggaggcttc gacggagcgc ctgtacccgc
      781 gtgacggtgt gctgaagggc gaaatccacc aggccctgaa gctgaaggat ggaggtcact
      841 acctcgtcga gttcaagacc atctacatgg ccaaaaagcc cgtgcagctc cccggctact
      901 actatgttga caccaagctc gacattacct cgcataacga ggactatacc atcgtggagc
      961 aatacgaacg ctcggagggc cggcaccacc tgttcctcgg tcatgggacc ggttcgactg
     1021 gcagcggtag ttcaggaacc gcctcgtcgg aggacaacaa tatggccgtg atcaaagagt
     1081 tcatgcgctt caaggtgcga atggaaggtt cgatgaacgg ccacgagttc gagatcgagg
     1141 gggaaggcga aggtcggcca tacgaaggca cccagacggc gaagttgaaa gtgaccaagg
     1201 gcggcccgct gccgttcgcg tgggacatcc tgtcaccgca attcatgtat ggctcgaagg
     1261 cctacgtcaa gcacccggcc gacattccag actacaagaa gctctctttt cccgagggct
     1321 tcaagtggga gcgcgtcatg aactttgagg atggtggcct ggtgaccgta acgcaggaca
     1381 gctccctcca ggacgggacc ctgatctata aagtgaagat gcgcggcacc aacttcccac
     1441 ccgatggccc agtaatgcaa aagaaaacga tgggatggga ggcaagcacc gaacgtctgt
     1501 acccgcgcga cggagtcctg aagggtgaaa tccaccaagc cctaaaactg aaggacggtg
     1561 ggcactacct ggttgagttc aagaccatct atatggcaaa gaagcccgtg caactccccg
     1621 gatattacta cgtggacacg aagctggaca tcaccagcca caacgaagac tacacgatcg
     1681 tcgagcaata cgaacggagt gagggtcggc accatctctt cctctatggt atggacgagc
     1741 tgtacaagta aaagcttatc gataccgtcg acctcgaggg ggggcccccg gggatcatcg
     1801 agccgagaac gttatcgaag ttggtcatgt gtaatcccct cgtttgaact ttggattaag
     1861 cgtagataca cccttggaca agccagttgg attcggagac aagcaaattc agccttaaaa
     1921 agggcgaggc cctgcggtgg tggaacaccg cagggcctct aaccgctcga cgcgctgcac
     1981 caaccagccc gcgaacggct ggcagccagc gtaaggcgcg gctcatcggg cggcgttcgc
     2041 cacgatgtcc tgcacttcga gccaagcctc gaacacctgc tggtgtgcac gactcacccg
     2101 gttgttgaca ccgcgcgcgg ccgtgcgggc tcggtggggc ggctctgtcg cccttgccag
     2161 cgtgagtagc gcgtacctca cctcgcccaa caggtcgcac acagccgatt cgtacgccat
     2221 aaagccaggt gagcccacca gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca
     2281 ttcaggcggc agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct
     2341 cagcggcccg gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt
     2401 tgggggtgct cggctgtcgc tggtgttcca ccaccagggc tcgacgggag agcgggggag
     2461 tgtgcagttg tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat
     2521 acaccggtga ttaatcgtgg tctactacca agcgtgagcc acgtcgccga cgaatttgag
     2581 cagctctggc tgccgtactg gccgctggca agcgacgatc tgctcgaggg gatctaccgc
     2641 caaagccgcg cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca
     2701 aacctgctgg tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg
     2761 gggtcccatc cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca
     2821 gtgtgggcac tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca
     2881 tacatggcgg cgtgcgccga aggccttcgg cgcgccgtcg acggcgaccg cagttactca
     2941 ggcctcatga ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat
     3001 ctctacacac tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg
     3061 cgtcagcaga ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc
     3121 gattccgtca ggttgtgggc ctatcgtccc gccctcatgc ggatctacct gccgacccgg
     3181 aacgtggacg gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattc
     3241 ccgtgcaacg acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac
     3301 agcatttggc gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac
     3361 gaggccacac tcagtgcgcg ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg
     3421 gcggcgagca cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc attgctatga
     3481 gcgacggcta cagcgacggc tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga
     3541 ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg
     3601 cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct
     3661 atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg ctgcatctgg
     3721 acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg
     3781 ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat tggggagcgg
     3841 gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg
     3901 tagacgctag ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt
     3961 tttgaatacc agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc
     4021 cgctgaatat cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac
     4081 tccgttgtag tgcctgtggt ggcatccgtg gccactctcg ttgcacggtt cgttgtgccg
     4141 ttacaggccc cgttgacagc tcaccgaacg tagttaaaac atgctggtca aactaggttt
     4201 accaacgata cgagtcagct catctagggc cagttctagg cgttgttcgt tgcgcggttc
     4261 gttgcgcatg tttcgtgtgg ttgctagatg gctccgcaac cacacgcttc gaggttgagt
     4321 gcttccagca cgggcgcgat ccagaagaac ttcgtcgtgc gactgtcctc gttgatcctt
     4381 gccgagctgg gatggaagcc cggccgaccc accctggagg agatgatcga ggatgccagg
     4441 gcctttcacg cccgccgctg ctgagcgtcc gccgccgggc ccgcaccgcc gtcggccggc
     4501 ccgctccggg ctcgcagcag cgggcttcgg cgcgggcccg gggctcccgg gccgccgggc
     4561 ggggctccgc ccggcggccg ccgggggccg ggggcggcgc cgggcggccc ggggcgtcag
     4621 gcgccggggg cggtgtccgg cggcccccag aggaactgcg ccagttcctc cggatcggtg
     4681 aagccggaga gatccagcgg ggtctcctcg aacacctcga agtcgtgcag gaaggtgaag
     4741 gcgagcagtt cgcgggcgaa gtcctcggtc cgcttccact gcgccccgtc gagcagcgcg
     4801 gccaggatct cgcggtcgcc ccggaaggcg ttgagatgca gttgcaccag gctgtagcgg
     4861 gagtctcccg catagacgtc ggtgaagtcg acgatcccgg tgacctcggt cgcggccagg
     4921 tccacgaaga tgttggtccc gtgcaggtcg ccgtggacga accggggttc gcggccggcc
     4981 agcagcgtgt ccacgtccgg cagccagtcc tccaggcggt ccagcagccg gggcgagagg
     5041 tagccccacc cgcggtggtc ctcgacggtc gccgcgcggc gttcccgcag cagttccggg
     5101 aagacctcgg aatggggggt gagcacggtg ttcccggtca gcggcaccct gtgcagccgg
     5161 ccgagcaccc ggccgagttc gcgggccagg gcgagcagcg cgttccggtc ggtcgtgccg
     5221 tccatcgcgg accgccaggt ggtgccggtc atccggctca tcaccaggta gggccacggc
     5281 caggctccgg tgccgggccg cagctcgccg cggccgagga ggcggggcac cggcaccggg
     5341 gcgtccgcca ggaccgcgta cgcctccgac tccgacgcga ggctctccgg accgcaccag
     5401 tgctcgccga acagcttgat caccgggccg ggctcgccga ccagtacggg gttggtgctc
     5461 tcgccgggca cccgcagcac cggcggcacc ggcagcccga gctcctccag ggctcggcgg
     5521 gccagcggct cccagaattc ctggtcgttc cgcaggctcg cgtaggaatc atccgaatca
     5581 atacggtcga gaagtaacag ggattcttgt gtcacagcgg acctctattc acagggtacg
     5641 ggccggctta attccgcacg gccggtcgcg acacggcctg tccgcaccgc ggatcaggcg
     5701 ttgacgatga cgggctggtc ggccacgtcg gggagtcagg caactatgga tgaacgaaat
     5761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6781 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6841 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6901 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6961 tatgacatga ttaccagatc tggctcgcac cgcggtggcg gccgc
//
