LOCUS       sequence-309842-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-EBFP2-ccdB-Puro
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(543..545,546..548,549..1259)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(588..609)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    1290..1414
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1439..1444,1445..1462,1463..1469)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1523..2182
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1589..1608)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2524..2829
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2708..2727
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2870..2994)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2999..3040
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3054..3071
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3081..3591
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3510..3528
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(3607..3626)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4103..4123
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4213..4266
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4267..4884,4885..4890,4891..4926,4927..4929,4930..4965,
                     4966..4968,4969..5004,5005..5013)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5053..5073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5471..5482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5687..5920
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5992..6113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6029..6048)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6083..6102
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6153..6288
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6288..6359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6360..6428,6429..7220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6578..6597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7391..7979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7880..7899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8133..8150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8267..8288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8303..8308,8309..8326,8327..8333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8341..8357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8346..8368
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8365..8381
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8365..8381
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8400..8420
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8402..8420
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8448..8674
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8675..8855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8902..9027
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9520..9753
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9938..9982
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      601 acggcgacgt aaacggccac aagttcagcg tgaggggcga gggcgagggc gatgccacca
      661 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      721 ccctcgtgac caccctgagc cacggcgtgc agtgcttcgc ccgctacccc gaccacatga
      781 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      841 tcttcaagga cgacggcacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      901 tagtgaaccg catcgagctg aagggcgtcg acttcaagga ggacggcaac atcctggggc
      961 acaagctgga gtacaacttc aacagccaca acatctatat catggccgtc aagcagaaga
     1021 acggcatcaa ggtgaacttc aagatccgcc acaacgtgga ggacggcagc gtgcagctcg
     1081 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacagcc
     1141 actacctgag cacccagtcc gtgctgagca aagaccccaa cgagaagcgc gatcacatgg
     1201 tcctgctgga gttccgcacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1261 gggccggcca tatcagcggc tctggttcga caagtttgta caaaaaagct gaacgagaaa
     1321 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1381 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1441 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1501 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1561 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1621 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1681 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1741 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1801 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1861 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1921 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1981 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2041 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2101 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2161 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     2221 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2281 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2341 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2401 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2461 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2521 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2581 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2641 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2701 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2761 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2821 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2881 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2941 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgcagg
     3001 aaagccaatc ccaaacccac tcctaggtct cgacagtaca gcatagaccg gtccaccacc
     3061 accaccacca ctaaggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     3121 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     3181 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     3241 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     3301 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     3361 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     3421 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     3481 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     3541 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gcttaattaa
     3601 ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     3661 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     3721 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     3781 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     3841 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     3901 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     3961 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     4021 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     4081 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     4141 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcctgca
     4201 ggtcccggtg ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct
     4261 ggtccaatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     4321 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     4381 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     4441 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     4501 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     4561 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     4621 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     4681 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     4741 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     4801 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     4861 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     4921 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     4981 gacgattttg accttgacat gctccccggg taactaagta aggatcgatc caagatatcg
     5041 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5101 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5161 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5221 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5281 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5341 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     5401 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5461 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5521 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5581 ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac
     5641 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     5701 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     5761 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5821 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5881 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     5941 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     6001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6121 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6181 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6241 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt
     6301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8161 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8281 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8341 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8401 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     8461 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     8521 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8581 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8641 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8821 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8881 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8941 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     9001 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     9061 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     9121 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     9181 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     9241 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9301 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9361 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9421 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9481 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9541 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9601 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9661 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9721 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9781 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9841 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9901 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9961 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10021 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10081 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10141 aaccccgagg ggac
//
