LOCUS       sequence-309844-        6282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson 
            BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
  TITLE     Sensitive detection of gene expression in mycobacteria under 
            replicating and non-replicating conditions using optimized far-red 
            reporters.
  JOURNAL   PLoS One. 2010  . 5(3):e9823.
  PUBMED    20352111
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(3):e9823."
COMMENT     Sequence Label: pCHARGE3
FEATURES             Location/Qualifiers
     source          1..6282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1037..1053)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1038..1054)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      5253..5841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5742..5761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5995..6012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6129..6150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6165..6170,6171..6188,6189..6195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6203..6219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6208..6230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6227..6243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6227..6243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctagaggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct ctgggagtac ccgtgtgtac
      241 gaccagcacg gcatacatca tttcgacgcc gagagattcg ccgcccgaaa tgagcacgat
      301 ccggatccag gagggaattc atggtcggcg aggacagcgt cctgatcacc gagaacatgc
      361 acatgaagct ctacatggag ggcaccgtca acgaccacca tttcaagtgt acctcggagg
      421 gtgagggcaa gccgtacgag ggcacccaga ccatgaagat caaggtcgtg gagggcggtc
      481 cgctgccgtt cgccttcgac atcctcgcca ccagcttcat gtacggttcg aagaccttca
      541 tcaaccacac ccagggcatc ccggacttct tcaagcaatc gttccccgag ggcttcacct
      601 gggagcgcat caccacctac gaggacggtg gcgtcctgac cgccacccag gacacctcgc
      661 tccagaacgg ctgcctgatc tacaacgtga agatcaacgg cgtcaacttc ccctcgaacg
      721 gcccggtcat gcagaagaag acgctgggct gggaggccag caccgagatg ctgtaccccg
      781 ccgactccgg tctgcgcggc cactcccaga tggcgctcaa gctggtgggt ggcggctacc
      841 tgcactgttc gctcaagacg acctaccgct cgaagaagcc ggcgaagaac ctgaagatgc
      901 ccggcttcta cttcgtggac cgccggctgg agcggatcaa ggaggcggac aaggagacct
      961 acgtggagca gcacgagatg gccgtcgccc gctactgcga cctgccctcg aagctgggcc
     1021 actcgtaaaa gcttatcgat accgtcgacc tcgagggggg gcccccgggg atcatcgagc
     1081 cgagaacgtt atcgaagttg gtcatgtgta atcccctcgt ttgaactttg gattaagcgt
     1141 agatacaccc ttggacaagc cagttggatt cggagacaag caaattcagc cttaaaaagg
     1201 gcgaggccct gcggtggtgg aacaccgcag ggcctctaac cgctcgacgc gctgcaccaa
     1261 ccagcccgcg aacggctggc agccagcgta aggcgcggct catcgggcgg cgttcgccac
     1321 gatgtcctgc acttcgagcc aagcctcgaa cacctgctgg tgtgcacgac tcacccggtt
     1381 gttgacaccg cgcgcggccg tgcgggctcg gtggggcggc tctgtcgccc ttgccagcgt
     1441 gagtagcgcg tacctcacct cgcccaacag gtcgcacaca gccgattcgt acgccataaa
     1501 gccaggtgag cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc
     1561 aggcggcagg gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag
     1621 cggcccggaa acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg
     1681 gggtgctcgg ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt
     1741 gcagttgtgg ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca
     1801 ccggtgatta atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag
     1861 ctctggctgc cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa
     1921 agccgcgcgt cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac
     1981 ctgctggtcg tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg
     2041 tcccatccgc tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg
     2101 tgggcactca acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac
     2161 atggcggcgt gcgccgaagg ccttcggcgc gccgtcgacg gcgaccgcag ttactcaggc
     2221 ctcatgacca aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc
     2281 tacacactca gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt
     2341 cagcagacca cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat
     2401 tccgtcaggt tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac
     2461 gtggacggac tcggccgcgc gatctatgcc gagtgccacg cgcgaaacgc cgaattcccg
     2521 tgcaacgacg tgtgtcccgg accgctaccg gacagcgagg tccgcgccat cgccaacagc
     2581 atttggcgtt ggatcacaac caagtcgcgc atttgggcgg acgggatcgt ggtctacgag
     2641 gccacactca gtgcgcgcca gtcggccatc tcgcggaagg gcgcagcagc gcgcacggcg
     2701 gcgagcacag ttgcgcggcg cgcaaagtcc gcgtcagcca tggaggcatt gctatgagcg
     2761 acggctacag cgacggctac aaccggcagc cgactgtccg caaaaagcgg cgcgtgaccg
     2821 ccgccgaagg cgctcgaatc accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc
     2881 aggaacgcag cgagtggctc gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc
     2941 acgacgacga gggccactct tggccgcaaa cggccaaaca tttcgggctg catctggaca
     3001 ccgttaagcg actcggctat cgggcgagga aagagcgtgc ggcagaacag gaagcggctc
     3061 aaaaggccca caacgaagcc gacaatccac cgctgttcta acgcaattgg ggagcgggtg
     3121 tcgcgggggt tccgtggggg gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag
     3181 acgctagttt tctggtttgg gccatgcctg tctcgttgcg tgtttcgttg cgcccgtttt
     3241 gaataccagc cagacgagac ggggttctac gaatcttggt cgataccaag ccatttccgc
     3301 tgaatatcgg ggagctcacc gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc
     3361 gttgtagtgc ctgtggtggc atccgtggcc actctcgttg cacggttcgt tgtgccgtta
     3421 caggccccgt tgacagctca ccgaacgtag ttaaaacatg ctggtcaaac taggtttacc
     3481 aacgatacga gtcagctcat ctagggccag ttctaggcgt tgttcgttgc gcggttcgtt
     3541 gcgcatgttt cgtgtggttg ctagatggct ccgcaaccac acgcttcgag gttgagtgct
     3601 tccagcacgg gcgcgatcca gaagaacttc gtcgtgcgac tgtcctcgtt gatccttgcc
     3661 gagctgggat ggaagcccgg ccgacccacc ctggaggaga tgatcgagga tgccagggcc
     3721 tttcacgccc gccgctgctg agcgtccgcc gccgggcccg caccgccgtc ggccggcccg
     3781 ctccgggctc gcagcagcgg gcttcggcgc gggcccgggg ctcccgggcc gccgggcggg
     3841 gctccgcccg gcggccgccg ggggccgggg gcggcgccgg gcggcccggg gcgtcaggcg
     3901 ccgggggcgg tgtccggcgg cccccagagg aactgcgcca gttcctccgg atcggtgaag
     3961 ccggagagat ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa ggtgaaggcg
     4021 agcagttcgc gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag cagcgcggcc
     4081 aggatctcgc ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct gtagcgggag
     4141 tctcccgcat agacgtcggt gaagtcgacg atcccggtga cctcggtcgc ggccaggtcc
     4201 acgaagatgt tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg gccggccagc
     4261 agcgtgtcca cgtccggcag ccagtcctcc aggcggtcca gcagccgggg cgagaggtag
     4321 ccccacccgc ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag ttccgggaag
     4381 acctcggaat ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg cagccggccg
     4441 agcacccggc cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt cgtgccgtcc
     4501 atcgcggacc gccaggtggt gccggtcatc cggctcatca ccaggtaggg ccacggccag
     4561 gctccggtgc cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg caccggggcg
     4621 tccgccagga ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc gcaccagtgc
     4681 tcgccgaaca gcttgatcac cgggccgggc tcgccgacca gtacggggtt ggtgctctcg
     4741 ccgggcaccc gcagcaccgg cggcaccggc agcccgagct cctccagggc tcggcgggcc
     4801 agcggctccc agaattcctg gtcgttccgc aggctcgcgt aggaatcatc cgaatcaata
     4861 cggtcgagaa gtaacaggga ttcttgtgtc acagcggacc tctattcaca gggtacgggc
     4921 cggcttaatt ccgcacggcc ggtcgcgaca cggcctgtcc gcaccgcgga tcaggcgttg
     4981 acgatgacgg gctggtcggc cacgtcgggg agtcaggcaa ctatggatga acgaaataga
     5041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6001 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6061 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6121 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6181 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6241 gacatgatta ccagatctgg ctcgcaccgc ggtggcggcc gc
//
