LOCUS       sequence-30985-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC,
            Brock JE, Richardson AL, Weinberg RA
  TITLE     A pleiotropically acting microRNA, miR-31, inhibits breast cancer 
            metastasis.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1032-46.
  PUBMED    19524507
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1032-46."
COMMENT     Sequence Label: pIS1-miR-335 site
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    208..329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(219..238)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     273..292
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        462..566
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
       61 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
      121 taggagctct caagagcaat aacgaaaaat gtaccggtct cgatatcact actagtgttc
      181 tagagcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
      241 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      301 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      361 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      421 aaatcgataa ggatccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      481 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      541 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      601 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      661 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggnt ctcaacagcg
      721 gtaagatcct tgaga
//
