LOCUS       sequence-309855-        9067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber 
            suppression in transient or stable, piggybac-mediated, integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Meineke B, Heimgartner J, Eirich J, Landreh M, Elsasser SJ
  TITLE     Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal
            Labeling and Crosslinking of Proteins on Live Mammalian Cells.
  JOURNAL   Cell Rep. 2020 Jun 23;31(12):107811. doi: 
            10.1016/j.celrep.2020.107811.
  PUBMED    32579937
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-23"; volume: "31(12):107811. doi"; pages: " 
            10.1016/j.celrep.2020.107811"
COMMENT     Sequence Label: pAS_4xG1 PylT FLAG-G1 PylRS Y125A
FEATURES             Location/Qualifiers
     source          1..9067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   288..350
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2154..2365
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2378..2646
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2678..2687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2687..2710
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3554..4126
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3721..3738)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3948..3967
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4135..4929
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4189..4208)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4799..4818
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4989..5009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5051..5172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5088..5107)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5142..5161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5935..5969
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6460..6476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6460..6476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6473..6495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6484..6500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6508..6514,6515..6532,6533..6538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6553..6574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6691..6708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6862..7450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6942..6961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7621..8412,8413..8481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8244..8263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8482..8586)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8612..9067)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8749..8770)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8961..8980
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg
      181 tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg
      241 agcagatgaa gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata
      301 atcatattgt gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg
      361 gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca
      421 cttacatact aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa
      481 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc
      541 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
      601 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
      661 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
      721 tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccacggcc aactagtaaa
      781 aaccgcactt gtccgaaagg ccggggggtc gaaccccgct tacaggtttt agagacccgt
      841 ttgctcgccg gagcgccctc cgaggtaccc aggcggcgca caagctatat aaacctgaag
      901 gaaatctcaa ctttacactt aggtcaagtt acttatcgta ctagagcttc agcaggaaat
      961 ttaactaaaa tctaatttaa ccagcatagc aaatatcatt tattcccaaa atgctaaagt
     1021 ttgagataaa cggacttgat ttccggctgt tttgacacta tccagaatgc cttgcagatg
     1081 ggtggggcat gctaaatact gcaggcaaaa aaaccgcact tgtccgaaag gccggggggt
     1141 cgaaccccgc ttacaggttt tagagacccg tttgctcgcc ggagcgccct ccgaggtacc
     1201 caggcggcgc acaagctata taaacctgaa ggaaatctca actttacact taggtcaagt
     1261 tacttatcgt actagagctt cagcaggaaa tttaactaaa atctaattta accagcatag
     1321 caaatatcat ttattcccaa aatgctaaag tttgagataa acggacttga tttccggctg
     1381 ttttgacact atccagaatg ccttgcagat gggtggggca tgctaaatac tgcagcaaga
     1441 aaaaccgcac ttgtccgaaa ggccgggggg tcgaaccccg cttacaggtt ttagagaccc
     1501 gtttgctcgc cggagcgccc tccgaggtac ccaggcggcg cacaagctat ataaacctga
     1561 aggaaatctc aactttacac ttaggtcaag ttacttatcg tactagagct tcagcaggaa
     1621 atttaactaa aatctaattt aaccagcata gcaaatatca tttattccca aaatgctaaa
     1681 gtttgtgata aacggacttg atttccggct gttttgacac tatccagaat gccttgcaga
     1741 tgggtggggc atgctaaata ctgcagcgat aaaaaccgca cttgtccgaa aggccggggg
     1801 gtcgaacccc gcttacaggt tttagagacc cgtttgctcg ccggagcgcc ctccgaggta
     1861 cccaggcggc gcacaagcta tataaacctg aaggaaatct caactttaca cttaggtcaa
     1921 gttacttatc gtactagagc ttcagcagga aatttaacta aaatctaatt taaccagcat
     1981 agcaaatatc atttattccc aaaatgctaa agtttgagat aaacggactt gatttccggc
     2041 tgttttgaca ctatccagaa tgccttgcag atgggtgggg catgctaaat actgcagctc
     2101 caaggccact agtgctctag aaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     2161 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     2221 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     2281 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     2341 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     2401 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     2461 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     2521 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     2581 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     2641 cagatccaag ctgtgaccgg cgcctactct agagctagcc accatggact acaaggacga
     2701 cgacgacaag gtggtcaagt tcaccgacag ccagatccag cacctgatgg aatacggcga
     2761 caacgattgg agcgaggccg agttcgaaga tgccgccgct agagacaaag agttcagcag
     2821 ccagttcagc aagctgaagt ccgccaacga caagggcctg aaggacgtga tcgccaatcc
     2881 tcggaacgac ctgaccgacc tggaaaacaa gatcagagag aagctggccg ccagaggctt
     2941 catcgaggtg cacaccccta tcttcgtgtc caagtctgcc ctggccaaga tgaccatcac
     3001 cgaggatcac cctctgttca aacaggtgtt ctggatcgac gacaagcggg ccctgaggcc
     3061 tatgcacgcc atgaacctgg ccaaagtgat gagagagctg cgggaccaca caaagggccc
     3121 cgtgaagatc tttgagatcg gcagctgctt ccggaaagag agcaagagca gcacccacct
     3181 ggaagagttc accatgctga acctggtgga aatgggcccc gacggcgatc ctatggaaca
     3241 cctgaagatg tatatcggcg acatcatgga cgccgtgggc gtcgagtaca ccaccagcag
     3301 agaagagtcc gacgtgtacg tggaaaccct ggacgtggaa atcaacggca ccgaagtggc
     3361 tagcggagcc gtgggacctc acaaactgga tcctgctcac gacgtgcacg aaccttgggc
     3421 cggaatcggc tttggcctgg aaagactgct gatgctgaag aacggcaaga gcaacgccag
     3481 aaagaccggc aagtccatca cctacctgaa cggctacaag ctggactgat gaatcgatgg
     3541 atccgcggcc gcgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     3601 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     3661 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     3721 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     3781 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     3841 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     3901 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     3961 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     4021 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     4081 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataatacctc cggaatgatt
     4141 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4201 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4261 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     4321 gaggcagcgc ggctatcgtg gctggccgcg acgggcgttc cttgcgcagc tgtgctcgac
     4381 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4441 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4501 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4561 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4621 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4681 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4741 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4801 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4861 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4921 ttcttctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4981 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5041 gcgttaacta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5101 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5161 caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct
     5221 catctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc
     5281 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc
     5341 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga
     5401 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg
     5461 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     5521 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     5581 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     5641 tttggggggg ggctgtccct gatatctata acaagaaaat atatatataa taagttatca
     5701 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     5761 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     5821 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     5881 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     5941 tcaattttac gcagactatc tttctagggt taatctagct gcatcaggat catatcgtcg
     6001 ggtctttttt ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag
     6061 aagcccgtgc cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact
     6121 gctgctgcat tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg
     6181 ccatgcacgc ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc
     6241 ggcttttccg gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata
     6301 ccggcgacag ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg
     6361 gatattacgt cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac
     6421 cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7261 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8581 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     8641 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8701 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8761 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8821 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8881 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8941 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     9001 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     9061 ggcgcgt
//
