LOCUS       sequence-309864-        5312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MlaF 1-246, tagless.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Kolich LR, Chang YT, Coudray N, Giacometti SI, MacRae MR, Isom GL, 
            Teran EM, Bhabha G, Ekiert DC
  TITLE     Structure of MlaFB uncovers novel mechanisms of ABC transporter 
            regulation.
  JOURNAL   Elife. 2020 Jun 30;9. pii: 60030. doi: 10.7554/eLife.60030.
  PUBMED    32602838
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-30"; volume: "9. pii: 60030. doi"; pages: " 
            10.7554/eLife.60030"
COMMENT     Sequence Label: pBEL1647
FEATURES             Location/Qualifiers
     source          1..5312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..286
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     209..228
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(1172..1189)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1342..1428
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1520..1547
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1566..1657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1658..1726,1727..2518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1876..1895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2560..3015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2647..2666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2857..2878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3126..3713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3614..3633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3867..3884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4050..4072
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4210..4229
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4409..5287)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
       61 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
      121 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
      181 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
      241 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg
      301 gctagccgaa gggtgaatta tggagcagtc tgtggcgaat ttagtcgata tgcgcgatgt
      361 cagttttacg cgtggcaatc gctgcatctt cgataatatt tccctgaccg tgccgcgagg
      421 gaagatcacg gcgatcatgg ggccatcggg catcggtaaa acgacgctac tccgtctgat
      481 tggcgggcaa atcgcaccag atcatggtga gatccttttc gatggtgaga atattccggc
      541 gatgtctcgt tcgcgcctgt atacagtgcg caaacggatg agcatgttat ttcagtccgg
      601 ggcgttgttc actgatatga acgtatttga caacgtcgcc tatccactgc gcgaacatac
      661 ccaacttccc gcgccattgt tgcatagtac ggtgatgatg aagctggagg ccgtggggct
      721 gcgtggagcg gctaaactaa tgccttctga actttccggt gggatggcgc ggcgtgcagc
      781 gctggcgcgt gcgattgcgc tggagccgga tctcatcatg tttgatgaac cttttgttgg
      841 gcaagatccc atcaccatgg gcgtactggt gaagctgatt tctgagctga acagcgcgct
      901 gggcgtgact tgtgtggtgg tttctcacga tgtgccggaa gtgttaagta ttgcggatca
      961 cgcctggatc ctggcggaca aaaaaattgt cgctcatggc agtgcccagg cgttgcaggc
     1021 gaatcctgat ccgcgcgtac gtcagtttct ggacgggtaa ggtaccaaat tccagaaaag
     1081 agacgctgaa aagcgtcttt tttcgttttg gtcctggtct gaggaatggt tgtaagcttg
     1141 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa
     1201 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca
     1261 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga
     1321 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1381 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     1441 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     1501 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     1561 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1621 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1681 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1741 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1801 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1861 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     1921 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1981 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     2041 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2101 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2161 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     2221 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2281 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2341 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2401 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2461 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2521 gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat
     2581 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2641 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2701 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2761 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2821 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2881 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     2941 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3001 taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggctcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3901 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtcatggc
     3961 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4021 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4081 gtcatcaccg aaacgcgcga ggcagaagga gatggcgccc aacagtcccc cggccacggg
     4141 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     4201 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     4261 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atcatcgatg
     4321 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
     4381 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
     4441 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     4501 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     4561 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     4621 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     4681 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     4741 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     4801 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     4861 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     4921 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     4981 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     5041 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     5101 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     5161 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     5221 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     5281 cagccatact tttcatactc ccgccattca ga
//
