LOCUS       sequence-309870-        9962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-EBFP2-ccdB-Blast
FEATURES             Location/Qualifiers
     source          1..9962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(332..334,335..337,338..1048)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(377..398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    1079..1203
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1228..1233,1234..1251,1252..1258)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1312..1971
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1378..1397)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2313..2618
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2497..2516
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2659..2783)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2788..2829
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2843..2860
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2870..3380
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3299..3317
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3396..3791
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3810..3863
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3864..4481,4482..4487,4488..4523,4524..4526,4527..4562,
                     4563..4565,4566..4601,4602..4610)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4650..4670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5068..5079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5284..5517
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5589..5710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5626..5645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5680..5699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5750..5885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5885..5956
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5957..6025,6026..6817)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6175..6194)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6988..7576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7477..7496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7730..7747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7864..7885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7900..7905,7906..7923,7924..7930)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7938..7954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7943..7965
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7962..7978
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7962..7978
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7997..8017
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7999..8017
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8045..8271
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8272..8452
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8499..8624
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9117..9350
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9535..9579
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9792..9909
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag catggtgagc aagggcgagg agctgttcac
      361 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      421 gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
      481 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtgca
      541 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      601 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct acaagacccg
      661 cgccgaggtg aagttcgagg gcgacaccct agtgaaccgc atcgagctga agggcgtcga
      721 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
      781 catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      841 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      901 cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg tgctgagcaa
      961 agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg ccgccgggat
     1021 cactctcggc atggacgagc tgtacaaggg ggccggccat atcagcggct ctggttcgac
     1081 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     1141 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     1201 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     1261 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     1321 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1381 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1441 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1501 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     1561 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1621 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1681 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1741 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1801 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1861 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1921 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga
     1981 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     2041 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     2101 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     2161 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     2221 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     2281 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     2341 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     2401 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2461 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     2521 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     2581 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2641 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     2701 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     2761 tcagctttct tgtacaaagt ggttgcagga aagccaatcc caaacccact cctaggtctc
     2821 gacagtacag catagaccgg tccaccacca ccaccaccac taaggatccg gggttggggt
     2881 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     2941 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     3001 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     3061 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     3121 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     3181 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     3241 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     3301 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     3361 tcaccgacct ctctccccag cttaattaat tcaccatggc caagcctttg tctcaagaag
     3421 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     3481 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     3541 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     3601 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     3661 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     3721 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     3781 tgtgggaggg ccctgcaggt cccggtgccg aaggtagagg ttctctcctc acttgtggtg
     3841 atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag
     3901 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     3961 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     4021 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     4081 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     4141 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     4201 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     4261 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     4321 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     4381 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     4441 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     4501 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     4561 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     4621 atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4801 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4861 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4921 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4981 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5041 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5101 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5161 atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt
     5221 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5281 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5341 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5401 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5461 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5521 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5581 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5641 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5701 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     5761 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5821 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5881 aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5941 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6001 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6061 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6121 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6181 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6241 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6301 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6361 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6421 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6481 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6541 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6601 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6661 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6721 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6781 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6841 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6901 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6961 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7021 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7081 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7141 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7201 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7261 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7321 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7381 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7441 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7501 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7561 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7621 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7681 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7741 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7801 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7861 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7921 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7981 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8041 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     8101 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     8161 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     8221 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     8281 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8341 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8401 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8461 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8521 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8581 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8641 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8701 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     8761 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8821 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8881 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     8941 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     9001 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9061 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9121 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9181 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9241 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9301 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9361 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9421 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9481 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9541 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9601 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9661 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9721 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     9781 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9841 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     9901 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     9961 tc
//
