LOCUS       sequence-309874-       11059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-EBFP2-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..11059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(332..334,335..337,338..1048)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     protein_bind    1079..1203
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1228..1233,1234..1251,1252..1258)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1312..1971
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1378..1397)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2313..2618
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2497..2516
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2659..2783)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2788..2829
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2843..2860
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2870..3380
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3299..3317
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(3396..3398,3399..3401,3402..4112)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4131..4184
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4185..4802,4803..4808,4809..4844,4845..4847,4848..4883,
                     4884..4886,4887..4922,4923..4931)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4971..4991)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5389..5400)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5605..5838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5910..6031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5947..5966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6001..6020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6071..6206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6206..6277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6278..6346,6347..7138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6496..6515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7309..7897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7798..7817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8051..8068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8185..8206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8221..8226,8227..8244,8245..8251)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8259..8275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8264..8286
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8283..8299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8283..8299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8318..8338
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8320..8338
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     mobile_element  8339..9106
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     promoter        9142..9368
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9369..9549
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9596..9721
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10214..10447
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10632..10676
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10889..11006
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag catggtgagc aagggcgagg agctgttcac
      361 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      421 gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
      481 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtgca
      541 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      601 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct acaagacccg
      661 cgccgaggtg aagttcgagg gcgacaccct agtgaaccgc atcgagctga agggcgtcga
      721 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
      781 catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      841 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      901 cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg tgctgagcaa
      961 agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg ccgccgggat
     1021 cactctcggc atggacgagc tgtacaaggg ggccggccat atcagcggct ctggttcgac
     1081 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     1141 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     1201 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     1261 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     1321 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1381 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1441 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1501 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     1561 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1621 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1681 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1741 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1801 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1861 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1921 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga
     1981 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     2041 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     2101 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     2161 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     2221 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     2281 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     2341 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     2401 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2461 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     2521 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     2581 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2641 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     2701 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     2761 tcagctttct tgtacaaagt ggttgcagga aagccaatcc caaacccact cctaggtctc
     2821 gacagtacag catagaccgg tccaccacca ccaccaccac taaggatccg gggttggggt
     2881 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     2941 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     3001 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     3061 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     3121 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     3181 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     3241 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     3301 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     3361 tcaccgacct ctctccccag cttaattaat tcaccatggt gagcaagggc gaggagctgt
     3421 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3481 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3541 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
     3601 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3661 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3721 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3781 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3841 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3901 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3961 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
     4021 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4081 ggatcactct cggcatggac gagctgtaca agcctgcagg tcccggtgcc gaaggtagag
     4141 gttctctcct cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca
     4201 agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga
     4261 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga
     4321 agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc
     4381 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat
     4441 accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa
     4501 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct
     4561 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat
     4621 tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta
     4681 tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct
     4741 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg
     4801 gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg
     4861 acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc
     4921 tccccgggta actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct
     4981 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5041 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5101 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5161 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5221 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5281 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5341 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5401 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5461 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg
     5521 cgtccggact agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5581 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5641 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5821 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5881 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6061 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6121 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6181 gtagtgagga ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc
     6241 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6301 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6361 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6421 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6481 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6541 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6601 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6661 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6721 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6781 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6841 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6901 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6961 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7021 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7081 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7141 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7201 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7261 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7321 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7381 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7441 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7501 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7561 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7621 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7681 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7741 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7861 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7921 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7981 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8041 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8101 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8161 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8221 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8281 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaagggg
     8341 taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat
     8401 gcagctccac cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc
     8461 tcagattcag gttatgccgc tcaattcgct gcgtatatcg cttgctgatt acgtgcagct
     8521 ttcccttcag gcgggattca tacagcggcc agccatccgt catccatatc accacgtcaa
     8581 agggtgacag caggctcata agacgcccca gcgtcgccat agtgcgttca ccgaatacgt
     8641 gcgcaacaac cgtcttccgg agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt
     8701 tagccccgac atagccccac tgttcgtcca tttccgcgca gacgatgacg tcactgcccg
     8761 gctgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt
     8821 gaggccaacg cccataatgc gggctgttgc ccggcatcca acgccattca tggccatatc
     8881 aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca gttgccatgt
     8941 tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc cgttacgcac
     9001 caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc
     9061 tcaaaaacac catcatacac taaatcagta agttggcagc atcaccacta aagggaacaa
     9121 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     9181 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     9241 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     9301 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9361 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9421 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9481 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9541 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9601 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9661 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9721 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9781 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9841 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9901 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     9961 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    10021 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    10081 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10141 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10201 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10261 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10321 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10381 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10441 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10501 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10561 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10621 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    10681 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
    10741 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
    10801 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggacaa ttctcgacct
    10861 cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    10921 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10981 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    11041 ggccgcgaat cgatatgtc
//
