LOCUS       sequence-309875-       10130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-EBFP2-ccdB-NatMx
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(332..334,335..337,338..1048)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(377..398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    1079..1203
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1228..1233,1234..1251,1252..1258)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1312..1971
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1378..1397)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2313..2618
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2497..2516
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2659..2783)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2788..2829
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2843..2860
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2870..3380
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3299..3317
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3390..3959
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             3978..4031
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4032..4649,4650..4655,4656..4691,4692..4694,4695..4730,
                     4731..4733,4734..4769,4770..4778)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4818..4838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5236..5247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5452..5685
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5757..5878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5794..5813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5848..5867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5918..6053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6053..6124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6125..6193,6194..6985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6343..6362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7156..7744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7645..7664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7898..7915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8032..8053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8068..8073,8074..8091,8092..8098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8106..8122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8111..8133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8130..8146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8130..8146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8165..8185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8167..8185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8213..8439
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8440..8620
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8667..8792
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9285..9518
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9703..9747
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9960..10077
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag catggtgagc aagggcgagg agctgttcac
      361 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      421 gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
      481 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtgca
      541 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      601 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct acaagacccg
      661 cgccgaggtg aagttcgagg gcgacaccct agtgaaccgc atcgagctga agggcgtcga
      721 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
      781 catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca agatccgcca
      841 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      901 cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg tgctgagcaa
      961 agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg ccgccgggat
     1021 cactctcggc atggacgagc tgtacaaggg ggccggccat atcagcggct ctggttcgac
     1081 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     1141 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     1201 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     1261 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     1321 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1381 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1441 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1501 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     1561 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1621 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1681 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1741 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1801 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1861 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1921 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga
     1981 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     2041 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     2101 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     2161 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     2221 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     2281 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     2341 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     2401 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2461 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     2521 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     2581 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     2641 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     2701 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     2761 tcagctttct tgtacaaagt ggttgcagga aagccaatcc caaacccact cctaggtctc
     2821 gacagtacag catagaccgg tccaccacca ccaccaccac taaggatccg gggttggggt
     2881 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     2941 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     3001 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     3061 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     3121 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     3181 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     3241 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     3301 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     3361 tcaccgacct ctctccccag cttaattaaa tgggtaccac tcttgacgac acggcttacc
     3421 ggtaccgcac cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca
     3481 ccaccgacac cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc
     3541 cggtggaccc gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg
     3601 acggggagga cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc
     3661 tggcgggctt cgtggttgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca
     3721 tcgaggtcgc cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga
     3781 cggagttcgc ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg
     3841 caccggcgat ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc
     3901 tgtacgacgg caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgccccc
     3961 ctgcaggtcc cggtgccgaa ggtagaggtt ctctcctcac ttgtggtgat gttgaagaaa
     4021 accctggtcc aatgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac
     4081 tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     4141 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     4201 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     4261 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
     4321 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     4381 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     4441 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     4501 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     4561 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     4621 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
     4681 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     4741 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat cgatccaaga
     4801 tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4861 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4921 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4981 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5041 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5101 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5161 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5221 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5281 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5341 gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta agaccaatga
     5401 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5461 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5521 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5581 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5641 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5701 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5761 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5821 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5881 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5941 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6001 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctttcaaa
     6061 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     8161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     8221 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     8281 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     8341 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     8401 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     8461 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8521 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8581 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     8641 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     8701 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     8761 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     8821 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     8881 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     8941 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     9001 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     9061 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     9121 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     9181 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9241 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9301 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9361 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9421 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9481 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9541 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     9601 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     9661 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     9721 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     9781 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     9841 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     9901 ctcccaaccc cgaggggaca attctcgacc tcgagacaaa tggcagtatt catccacaat
     9961 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
    10021 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
    10081 gtttattaca gggacagcag agatccactt tggccgcgaa tcgatatgtc
//
