LOCUS       sequence-309876-        9215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-ccdB-Blast
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    332..456
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(481..486,487..504,505..511)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             565..1224
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(631..650)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1566..1871
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1750..1769
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1912..2036)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2041..2082
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2096..2113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2123..2633
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2552..2570
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2649..3044
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3063..3116
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3117..3734,3735..3740,3741..3776,3777..3779,3780..3815,
                     3816..3818,3819..3854,3855..3863)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3903..3923)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4321..4332)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4537..4770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4842..4963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4879..4898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4933..4952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5003..5138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5138..5209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5210..5278,5279..6070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5428..5447)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6241..6829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6730..6749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6983..7000
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7117..7138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7153..7158,7159..7176,7177..7183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7191..7207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7196..7218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7215..7231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7215..7231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7250..7270
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7252..7270
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7298..7524
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7525..7705
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7752..7877
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8370..8603
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8788..8832
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9045..9162
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag cacaagtttg tacaaaaaag ctgaacgaga
      361 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      421 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
      481 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
      541 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      601 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      661 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      721 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      781 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
      841 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
      901 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
      961 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1021 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1081 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1141 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1201 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     1261 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     1321 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     1381 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     1441 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     1501 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     1561 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     1621 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     1681 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     1741 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     1801 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     1861 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     1921 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     1981 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgca
     2041 ggaaagccaa tcccaaaccc actcctaggt ctcgacagta cagcatagac cggtccacca
     2101 ccaccaccac cactaaggat ccggggttgg ggttgcgcct tttccaaggc agccctgggt
     2161 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     2221 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     2281 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     2341 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     2401 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     2461 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     2521 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     2581 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcttaatt
     2641 aattcaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     2701 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     2761 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     2821 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     2881 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     2941 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     3001 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggccctgca ggtcccggtg
     3061 ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt
     3121 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     3181 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     3241 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     3301 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     3361 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     3421 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     3481 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     3541 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     3601 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     3661 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     3721 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     3781 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     3841 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     3901 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3961 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4021 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4081 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4141 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4201 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4261 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4321 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4381 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4441 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     4501 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4561 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4621 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4681 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4741 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     4801 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     4861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4981 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5101 gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat
     5161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5221 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5281 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5341 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5401 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5461 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5521 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5581 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5641 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5701 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5761 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5821 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5881 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5941 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6001 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6061 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6121 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6361 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6901 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6961 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7081 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7141 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7201 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7261 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7321 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7381 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7441 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     7501 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     7561 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7621 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7681 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     7741 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     7801 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     7861 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     7921 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     7981 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8041 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8101 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8161 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8221 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8281 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8341 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8401 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8461 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8521 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8581 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8641 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     8701 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     8761 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     8821 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     8881 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     8941 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9001 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     9061 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9121 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     9181 agcagagatc cactttggcc gcgaatcgat atgtc
//
