LOCUS       sequence-309878-        9383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-ccdB-NatMx
FEATURES             Location/Qualifiers
     source          1..9383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    332..456
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(481..486,487..504,505..511)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             565..1224
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(631..650)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1566..1871
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1750..1769
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1912..2036)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2041..2082
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2096..2113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2123..2633
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2552..2570
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2643..3212
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             3231..3284
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3285..3902,3903..3908,3909..3944,3945..3947,3948..3983,
                     3984..3986,3987..4022,4023..4031)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4071..4091)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4489..4500)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4705..4938
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5010..5131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5047..5066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5101..5120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5171..5306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5306..5377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5378..5446,5447..6238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5596..5615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6409..6997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6898..6917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7151..7168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7285..7306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7321..7326,7327..7344,7345..7351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7359..7375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7364..7386
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7383..7399
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7383..7399
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7418..7438
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7420..7438
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7466..7692
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7693..7873
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7920..8045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8538..8771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8956..9000
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9213..9330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag cacaagtttg tacaaaaaag ctgaacgaga
      361 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      421 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
      481 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
      541 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      601 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      661 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      721 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      781 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
      841 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
      901 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
      961 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1021 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1081 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1141 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1201 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     1261 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     1321 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     1381 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     1441 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     1501 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     1561 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     1621 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     1681 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     1741 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     1801 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     1861 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     1921 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     1981 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgca
     2041 ggaaagccaa tcccaaaccc actcctaggt ctcgacagta cagcatagac cggtccacca
     2101 ccaccaccac cactaaggat ccggggttgg ggttgcgcct tttccaaggc agccctgggt
     2161 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     2221 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     2281 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     2341 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     2401 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     2461 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     2521 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     2581 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcttaatt
     2641 aaatgggtac cactcttgac gacacggctt accggtaccg caccagtgtc ccgggggacg
     2701 ccgaggccat cgaggcactg gatgggtcct tcaccaccga caccgtcttc cgcgtcaccg
     2761 ccaccgggga cggcttcacc ctgcgggagg tgccggtgga cccgcccctg accaaggtgt
     2821 tccccgacga cgaatcggac gacgaatcgg acgacgggga ggacggcgac ccggactccc
     2881 ggacgttcgt cgcgtacggg gacgacggcg acctggcggg cttcgtggtt gtctcgtact
     2941 ccggctggaa ccgccggctg accgtcgagg acatcgaggt cgccccggag caccgggggc
     3001 acggggtcgg gcgcgcgttg atggggctcg cgacggagtt cgcccgcgag cggggcgccg
     3061 ggcacctctg gctggaggtc accaacgtca acgcaccggc gatccacgcg taccggcgga
     3121 tggggttcac cctctgcggc ctggacaccg ccctgtacga cggcaccgcc tcggacggcg
     3181 agcaggcgct ctacatgagc atgccctgcc cccctgcagg tcccggtgcc gaaggtagag
     3241 gttctctcct cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca
     3301 agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga
     3361 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga
     3421 agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc
     3481 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat
     3541 accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa
     3601 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct
     3661 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat
     3721 tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta
     3781 tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct
     3841 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg
     3901 gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg
     3961 acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc
     4021 tccccgggta actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct
     4081 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4141 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4201 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4261 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4321 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4381 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4441 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4501 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4561 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg
     4621 cgtccggact agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4681 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4741 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4801 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4861 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4921 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     4981 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5041 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5101 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5161 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5221 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5281 gtagtgagga ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc
     5341 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5401 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5461 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5521 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5581 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5641 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5701 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5761 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5821 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5881 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5941 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6001 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6061 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7021 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7081 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7141 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7201 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7261 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7321 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7381 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     7441 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     7501 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     7561 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     7621 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     7681 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7741 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7801 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7861 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     7921 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     7981 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8041 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8101 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8161 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8221 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8281 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     8341 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     8401 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     8461 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     8521 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     8581 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     8641 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     8701 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     8761 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     8821 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     8881 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8941 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9001 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9061 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9121 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acaattctcg
     9181 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     9241 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     9301 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     9361 ctttggccgc gaatcgatat gtc
//
