LOCUS       sequence-30990-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Ivan M, Kondo K, Yang H, Kim W, Valiando J, Ohh M, Salic A, Asara 
            JM, Lane WS, Kaelin WG
  TITLE     HIFalpha targeted for VHL-mediated destruction by proline 
            hydroxylation: implications for O2 sensing.
  JOURNAL   Science. 2001 Apr 20. 292(5516):464-8.
  PUBMED    11292862
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2001 Apr 20. 292(5516):464-8."
COMMENT     Sequence Label: Gal4-HA-HIF1-alpha(530-652)
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..39
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             19..45
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(472..490)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(473..490)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(510..527)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    516..740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(873..892)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggatcatgg cttacccata cgatgttcca gattacgcta gcttgggtgg tcatatggcc
       61 atggaggccc cggggatccc catggaattc aagttggaat tggtagaaaa actttttgct
      121 gaagacacag aagcaaagaa cccattttct actcaggaca cagatttaga cttggagatg
      181 ttagctccct atatcccaat ggatgatgac ttccagttac gttccttcga tcagttgtca
      241 ccattagaaa gcagttccgc aagccctgaa agcgcaagtc ctcaaagcac agttacagta
      301 ttccagcaga ctcaaataca agaacctact gctaatgcca ccactaccac tgccaccact
      361 gatgaattaa aaacagtgac aaaagaccgt atggaagaca ttaaaatatt gattgcatct
      421 ccatctccta cccacataca taaagaaact acttgatcta gagggcccta ttctatagtg
      481 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
      781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      901 cgccacgttc gccg
//
