LOCUS       sequence-309910-        5666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimerization-dependent fluorescent protein binding partner targeted 
            to outer mitochondrial membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK
  TITLE     Fission and fusion machineries converge at ER contact sites to 
            regulate mitochondrial morphology.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 
            10.1083/jcb.201911122.
  PUBMED    32328629
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133843. doi"; 
            pages: " 10.1083/jcb.201911122"
COMMENT     Sequence Label: B-MFF
FEATURES             Location/Qualifiers
     source          1..5666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(655..673)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     923..942
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1117..1140
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2394..2515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2431..2450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2485..2504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2522..2977)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2659..2680)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2871..2890
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3004..3108
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3110..3467
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3110..3301
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3133..3153)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3318..3453
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3502..4296
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3502..4296
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3556..3575)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4166..4185
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4484..4503)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4528..4575
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4904..5492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5393..5412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      541 ccaccatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
      601 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      661 tgaaggtgac caagggcggc cccctgccct ttgcctggga catcctgtcc ccccagatca
      721 tgtacggctc caaagcgtac gtgaggcacc ccgccgacat ccccgattac aagaagctgc
      781 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaagacggc ggtctggtga
      841 ccgttaccca ggactcctcc ctgcaggacg gcacgctgat ctgcaaggtg aagattcgcg
      901 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      961 ccaccgagat gctgtacccc aaggacggcg tgctgaaggg ccatagctat caggccctga
     1021 agctgaagga cggcggccac tacctggtgg agttcgagac catctacatg gccaagaagc
     1081 ccgtgcaact gcccggcgat tactgtgtgg acaccaagct ggacatcacc tcccacaacg
     1141 aggactacac catcgtggaa cagtacgggc gctccgaggg ccgccaccgt ctgggcatgg
     1201 acgagcggta caagtccgga ctcagatctc gagtgatgag taaaggaaca agcagtgaca
     1261 catcactagg aagggtgagc agggcagcat ttccttctcc cactgctgct gagatggcag
     1321 aaattagtcg aattcagtac gaaatggaat atactgaagg cattagtcag cgaatgaggg
     1381 tcccagaaaa gttaaaagta gcaccgccaa acgctgacct ggaacaagga ttccaagaag
     1441 gagttccaaa tgctagtgtg ataatgcaag ttccggagag gattgttgta gcaggaaata
     1501 atgaagatgt ttcattttca agaccagcag atcttgacct tattcagtca actcccttta
     1561 aacccctggc actgaaaaca ccacctcgtg tacttacgct gagtgaaaga ccactagatt
     1621 ttctggattt agaaagacct cctacaaccc ctcaaaatga agaaatccga gcagttggca
     1681 gactaaaaag agagcggtct atgagtgaaa atgctgttcg ccaaaatgga cagctggtca
     1741 gaaatgattc tctgtggcac agatcagatt ctgccccaag aaataaaatt tcaaggttcc
     1801 aggcaccgat ttctgcaccg gagtacactg tgacaccatc gccacaacag gctcgggtct
     1861 gtcctcccca tatgttacct gaagatggag ctaatctttc ctctgctcgt ggcattttgt
     1921 cgcttatcca gtcttctact cgtagggcat accagcagat cttggatgtg ctggatgaaa
     1981 atcgcagacc tgtgttgcgt ggtgggtctg ctgccgccac ttctaatcct catcatgaca
     2041 acgtcaggta tggcatttca aatatagata caaccattga aggaacgtca gatgacctga
     2101 ctgttgtaga tgcagcttca ctaagacgac agataatcaa actaaataga cgtctacaac
     2161 ttctggaaga ggagaacaaa gaacgtgcta aaagagaaat ggtcatgtat tcaattactg
     2221 tagctttctg gctgcttaat agctggctct ggtttcgccg ctagggatcc accggatcta
     2281 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2341 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2401 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2461 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     2521 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2581 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2641 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2701 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2761 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2821 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2881 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2941 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3001 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3061 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     3121 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3301 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     3481 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3541 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3601 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3661 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     3721 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3781 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3841 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3901 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3961 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4021 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     4081 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4141 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4201 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4261 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4321 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4381 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4441 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     4501 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     4561 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     4621 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     4681 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     4741 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4801 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5521 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5581 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     5641 gttcatagcc catatatgga gttccg
//
