LOCUS       sequence-309912-        5681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dimerization-dependent green fluorescent protein targeted to 
            mitochondrial outer membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK
  TITLE     Fission and fusion machineries converge at ER contact sites to 
            regulate mitochondrial morphology.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 
            10.1083/jcb.201911122.
  PUBMED    32328629
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133843. doi"; 
            pages: " 10.1083/jcb.201911122"
COMMENT     Sequence Label: GA-MFF
FEATURES             Location/Qualifiers
     source          1..5681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(670..688)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     938..957
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1132..1155
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2409..2530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2446..2465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2500..2519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2537..2992)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2674..2695)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2886..2905
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3019..3123
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3125..3482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3125..3316
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3148..3168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3333..3468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3517..4311
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3517..4311
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3571..3590)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4181..4200
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4499..4518)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4543..4590
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4919..5507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5408..5427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      541 tgagcaagag cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc ttggagggct
      601 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgccct tacgagggca
      661 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      721 tgtcccccct gatcatgtac ggctccaaga tgtacgtgaa gcaccccgcc gacgtccccg
      781 attacatgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg cacttcgagg
      841 acggcggtct ggtgaccgcg acacaggact cctccctgca ggacggcacg ctgatctaca
      901 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacct
      961 tgggctggga ttatgccacc gagcgcctgt accccgaaga aggcgtgctg aagggcgagc
     1021 tcctggggcg cctgaagctg aaggacggcg gcctcaacct ggtggagtcc aagaccatct
     1081 acatggccaa gaagcccgtg caactgcccg gctactactt cgtggacacc aagctggaca
     1141 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     1201 accacctggg gcatggcacc ggcagcacct ccggactcag atctcgagtg atgagtaaag
     1261 gaacaagcag tgacacatca ctaggaaggg tgagcagggc agcatttcct tctcccactg
     1321 ctgctgagat ggcagaaatt agtcgaattc agtacgaaat ggaatatact gaaggcatta
     1381 gtcagcgaat gagggtccca gaaaagttaa aagtagcacc gccaaacgct gacctggaac
     1441 aaggattcca agaaggagtt ccaaatgcta gtgtgataat gcaagttccg gagaggattg
     1501 ttgtagcagg aaataatgaa gatgtttcat tttcaagacc agcagatctt gaccttattc
     1561 agtcaactcc ctttaaaccc ctggcactga aaacaccacc tcgtgtactt acgctgagtg
     1621 aaagaccact agattttctg gatttagaaa gacctcctac aacccctcaa aatgaagaaa
     1681 tccgagcagt tggcagacta aaaagagagc ggtctatgag tgaaaatgct gttcgccaaa
     1741 atggacagct ggtcagaaat gattctctgt ggcacagatc agattctgcc ccaagaaata
     1801 aaatttcaag gttccaggca ccgatttctg caccggagta cactgtgaca ccatcgccac
     1861 aacaggctcg ggtctgtcct ccccatatgt tacctgaaga tggagctaat ctttcctctg
     1921 ctcgtggcat tttgtcgctt atccagtctt ctactcgtag ggcataccag cagatcttgg
     1981 atgtgctgga tgaaaatcgc agacctgtgt tgcgtggtgg gtctgctgcc gccacttcta
     2041 atcctcatca tgacaacgtc aggtatggca tttcaaatat agatacaacc attgaaggaa
     2101 cgtcagatga cctgactgtt gtagatgcag cttcactaag acgacagata atcaaactaa
     2161 atagacgtct acaacttctg gaagaggaga acaaagaacg tgctaaaaga gaaatggtca
     2221 tgtattcaat tactgtagct ttctggctgc ttaatagctg gctctggttt cgccgctagg
     2281 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     2341 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2401 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2461 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2521 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2581 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2641 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2701 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2761 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2821 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2881 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2941 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     3001 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3061 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3121 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     3181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     3241 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3301 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3361 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3421 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3481 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     3541 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     3601 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     3661 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     3721 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     3781 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     3841 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     3901 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     3961 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     4021 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     4081 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     4141 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     4201 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     4261 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     4321 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     4381 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4441 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     4501 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     4561 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     4621 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     4681 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     4741 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     4801 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4861 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5041 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5221 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5281 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5341 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5401 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5461 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5521 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5581 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     5641 attacggggt cattagttca tagcccatat atggagttcc g
//
