LOCUS       sequence-309930-        8508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of mGold and EBFP2 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Lee J, Liu Z, Suzuki PH, Ahrens JF, Lai S, Lu X, Guan S, St-Pierre F
  TITLE     Versatile phenotype-activated cell sorting.
  JOURNAL   Sci Adv. 2020 Oct 23;6(43). pii: 6/43/eabb7438. doi: 
            10.1126/sciadv.abb7438. Print 2020 Oct.
  PUBMED    33097540
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-23"; volume: "6(43). pii: 6/43/eabb7438. doi"; pages:
            " 10.1126/sciadv.abb7438. Print 2020 Oct"
COMMENT     Sequence Label: pJL2-mGold-P2A-EBFP2
FEATURES             Location/Qualifiers
     source          1..8508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(704..706,707..709,710..1420)
                     /codon_start=1
                     /product="photostable derivative of mVenus (Lee et al., 
                     2020)"
                     /label=mGold
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTSLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1436..1492
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1499..1501,1502..1504,1505..2218)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(2220..2236)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2220..2236)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    2240..2464
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2478..2497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2479..2497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2645..2832
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2700..2724)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2844..2861)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2844..2860)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2853..2875)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3296..4642
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4753..5556)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5557..5772)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5797..5815)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5883..5987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5988..6056,6057..6848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6206..6225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7019..7607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7508..7527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7761..7778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7895..7916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7931..7936,7937..7954,7955..7961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7969..7985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7974..7996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7993..8009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7993..8009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8039..8494)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8176..8197)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8388..8407
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccccgaa ttcatatgtc tagaattaga tctatggtga gcaagggcga
      721 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      781 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      841 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccagcctggg
      901 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      961 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1021 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1081 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1141 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1201 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     1261 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1321 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1381 cgccgccggg atcactctcg gcatggacga gctgtacaag ctgcagggct ctggagccac
     1441 caacttcagc ctgctgaagc aggcaggcga cgtggaagag aaccctggcc ctgtcgacat
     1501 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1561 cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg
     1621 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1681 cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     1741 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1801 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1861 gaaccgcatc gagctgaagg gcgtggactt caaggaggac ggcaacatcc tggggcacaa
     1921 gctggagtac aacttcaaca gccacaacat ctatatcatg gccgtcaagc agaagaacgg
     1981 catcaaggtg aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
     2041 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acagccacta
     2101 cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2161 gctggagttc cgcaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg
     2221 atccactagt tctagacgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2281 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2341 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2401 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2461 atggggtacc caattcgccc tatagtgagt cgtattacgc gcgcatccag ctttggactt
     2521 cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga
     2581 aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa
     2641 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     2701 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     2761 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     2821 cacctctacc ggcatgccaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2881 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2941 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3001 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataatcgga
     3061 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     3121 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     3181 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     3241 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     3301 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     3361 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     3421 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     3481 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     3541 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     3601 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     3661 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     3721 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     3781 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     3841 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     3901 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     3961 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     4021 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     4081 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     4141 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     4201 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     4261 agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     4321 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     4381 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     4441 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     4501 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     4561 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     4621 tcatttcctt tgatattgga tcgatccgat gataagctgt caaacatgag aattgggtaa
     4681 taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta
     4741 taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt
     4801 tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac
     4861 aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa
     4921 tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc
     4981 atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt
     5041 cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa
     5101 ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa
     5161 accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc
     5221 tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt
     5281 tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt
     5341 gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg
     5401 acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca
     5461 caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg
     5521 agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcatgt
     5581 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     5641 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     5701 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     5761 tgatgaattg aaaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     5821 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5881 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5941 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6001 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6061 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6121 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6181 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6241 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6301 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6361 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6421 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6481 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6541 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6601 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6661 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7141 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7741 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7801 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7861 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7921 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7981 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttacc gcatcaggaa
     8041 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8101 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8161 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8221 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8281 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8341 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8401 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8461 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gcgctagc
//
