LOCUS       sequence-309935-        8930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the auxin receiver that report on auxin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Ma Y, Budde MW, Mayalu MN, Zhu J, Lu AC, Murray RM, Elowitz MB
  TITLE     Synthetic mammalian signaling circuits for robust cell population 
            control.
  JOURNAL   Cell. 2022 Mar 17;185(6):967-979.e12. doi: 
            10.1016/j.cell.2022.01.026. Epub 2022 Mar 1.
  PUBMED    35235768
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.01.026"; journalName: "Cell"; date: 
            "2022-03-17- 17"; volume: "185"; issue: "6"; pages: "967-979.e12"
COMMENT     Sequence Label: vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
FEATURES             Location/Qualifiers
     source          1..8930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        268..1446
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          499..1437
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1394..1414
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1469..3193
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             3194..3247
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3248..3952
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3390..3408)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3658..3677
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3974..4177
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             4190..4213
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4229..4627
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4629..4750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4666..4685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4720..4739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5273..5307
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5798..5814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5798..5814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5811..5833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5822..5838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5846..5852,5853..5870,5871..5876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5891..5912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6029..6046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6200..6788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6280..6299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6959..7750,7751..7819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7582..7601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7820..7924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7950..8405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8087..8108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8299..8318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8532..8554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8546..8563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8547..8563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8693..8755
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
       61 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc
      121 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
      181 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaggcctcca
      241 cggccagcac ctgcgtgacg tacgtgaggc tccggtgccc gtcagtgggc agagcgcaca
      301 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      361 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      421 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      481 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      541 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
      601 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
      661 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
      721 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
      781 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
      841 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
      901 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
      961 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     1021 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     1081 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     1141 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     1201 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     1261 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     1321 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     1381 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     1441 tcgtgaacct tcacgcaggt gggccaccat gacatacttt cctgaagagg tcgtcgaaca
     1501 catttttagc ttcctgcctg cacagagaga tagaaacaca gtgagcctgg tctgcaaagt
     1561 gtggtacgag atcgaacgcc tgagccggag aggagtgttc gtcggcaact gctatgctgt
     1621 gagagcaggc agggtcgccg ctaggtttcc aaatgtgcgc gcactgaccg tcaaggggaa
     1681 accccacttc gccgacttta acctggtgcc ccctgattgg ggaggatacg ccggcccttg
     1741 gatcgaggca gccgctcgcg gctgtcatgg actggaggaa ctgcgcatga agcgaatggt
     1801 ggtctctgac gaaagtctgg agctgctggc tcggagcttc cctaggtttc gcgcactggt
     1861 gctgatttct tgcgaaggct tcagcaccga tggactggca gccgtggcct cccactgtaa
     1921 gctgctgcgg gagctggacc tccaggagaa tgaagtggag gatagaggcc ccagatggct
     1981 gtcttgcttc ccagactcat gtaccagcct ggtgtccctg aactttgcct gcatcaaagg
     2041 cgaagtgaat gctgggtccc tggagcggct ggtctcaaga agccccaacc tgaggtctct
     2101 gcggctgaac cggagcgtga gcgtggacac tctggctaag attctgctga gaacccctaa
     2161 cctggaggat ctgggaaccg gcaatctgac agacgatttc cagacagaat cctactttaa
     2221 actgacttct gccctggaga agtgtaaaat gctgaggagt ctgtcaggat tctgggatgc
     2281 ttcacccgtg tgcctgagct ttatctaccc tctgtgtgca cagctgacag gcctgaacct
     2341 gagctatgca ccaaccctgg acgccagtga tctgacaaag atgatctcac gctgcgtgaa
     2401 actccagcga ctgtgggtgc tggactgtat ttccgataag gggctccagg tggtcgccag
     2461 ctcctgcaag gacctccagg agctgagagt gttcccatct gatttttacg tggccggata
     2521 tagtgctgtc actgaggaag gcctggtggc agtctcactg ggatgcccaa agctgaacag
     2581 cctgctgtat ttctgtcatc agatgactaa tgctgcactg gtgaccgtcg ccaagaactg
     2641 ccctaatttc acccgatttc ggctgtgtat tctggaacca ggcaaacccg acgtggtcac
     2701 atcccagcca ctggatgaag ggtttggagc tatcgtgaga gagtgcaagg gactccagag
     2761 gctgagcatt tccggcctgc tgacagacaa agtgttcatg tacatcggca agtatgctaa
     2821 gcagctggag atgctgagca ttgcatttgc cggagactcc gataagggca tgatgcacgt
     2881 gatgaacggg tgtaagaatc tgcgaaaact ggaaatccgg gacagccctt tcggggatgc
     2941 cgctctgctg ggaaactttg ccagatacga gacaatgagg agcctgtgga tgtctagttg
     3001 caatgtgact ctgaagggct gtcaggtcct ggctagtaaa atgcctatgc tgaacgtgga
     3061 agtcattaat gagcgggacg ggtctaacga aatggaggaa aatcatggcg acctgccaaa
     3121 ggtggagaaa ctgtatgtgt atcggaccac cgcaggggca agagatgatg ctcccaactt
     3181 tgtgaagatt ctggagggca gggggagcct cctaacttgc ggagacgtcg aggagaatcc
     3241 tggcccagtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3301 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3361 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3421 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3481 gcaccccgcc gacattcccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3541 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3601 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3661 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3721 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3781 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     3841 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     3901 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtctggatc
     3961 cggtgcaggc gccaaggaga agagtgcttg tcctaaagat ccagccaaac ctccggccaa
     4021 ggcacaagtt gtgggatggc caccggtgag atcataccgg aagaacgtga tggtttcctg
     4081 ccaaaaatca agcggtggcc cggaggcggc ggcgttcgtg aaggtatcaa tggacggagc
     4141 accgtacttg aggaaaatcg atttgaggat gtataaagga ggatccggtg actataagga
     4201 cgatgacgac aagggtggtg gagggtccat ggccaagcct ttgtctcaag aagaatccac
     4261 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag attacagcgt
     4321 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     4381 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     4441 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     4501 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     4561 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     4621 gggctgacaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4681 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4741 atgtatctta ggcccattgg tatggctttt tccccgtatc cccccaggtg tctgcaggct
     4801 caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg
     4861 gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc
     4921 ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt
     4981 tggggggggg ctgtccctga tatctataac aagaaaatat atatataata agttatcacg
     5041 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     5101 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     5161 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     5221 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     5281 aattttacgc agactatctt tctagggtta atctagctgc atcaggatca tatcgtcggg
     5341 tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa
     5401 gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga ggatgactgc
     5461 tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga aggtgtggcc
     5521 atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag ttcgtcgcgg
     5581 cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc
     5641 ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc ggcgctggga
     5701 tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac atggataccc
     5761 cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5881 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5941 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6601 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7081 gtgctgcaat gataccgcga gatccacgct caccggctcc agatttatca gcaataaacc
     7141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7921 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     7981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8401 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8461 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8521 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat
     8581 tcacgttttt gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt
     8641 gatggagcag atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa
     8701 agataatcat attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt
     8761 tatcggtctg tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat
     8821 ttacacttac atactaataa taaattcaac aaacaattta tttatgttta tttatttatt
     8881 aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttat
//
