LOCUS       sequence-309952-       15210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with OsCDPK2 intron, NU structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15210)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL080
FEATURES             Location/Qualifiers
     source          1..15210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        108..2105
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3605..3680
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4229..6613
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6626..6646
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6657..6905
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6926..6950
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8251..8880
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9309..10382
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10448..10642
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10878..10900)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10976..11116
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11031..11050
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11131..11148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11302..11890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11382..11401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11977..12771)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13196..13220
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13298..13472
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(join(14562..15210,1..28))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14605..14625)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
       61 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ggcgcgcctg cagtgcagcg
      121 tgacccggtc gtgcccctct cttgagataa ggtgagcatt gcatgtctaa gttataaaaa
      181 attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat
      241 atatttaaac tttactctac gaataatata atctatagta ctacaataat atcagtgttt
      301 tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca
      361 acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata
      421 gcttcaccta tataatactt catccatttt attagtacat ccatttaggg tttagggtta
      481 atggttttta tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat
      541 taagaaaact aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat
      601 aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa
      661 catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg
      721 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat
      781 ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc
      841 tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc
      901 tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct
      961 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1021 cgtgttgttc ggagcgcaca cacacacaac cagaactccc ccaaatccac ccgtcggcac
     1081 ctccgcttca aggtacgccg ctcgtcctcc cccccccccn tctctacctt ctcaagatcg
     1141 gcgttccggt ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc
     1201 cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg gatgcgacct gtacgtcaga
     1261 cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc
     1321 cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt
     1381 gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc
     1441 ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcaa gatcggagta
     1501 gaattaattc tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg
     1561 ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg
     1621 tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt
     1681 tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc aagatcggag tagaatactg
     1741 tttcaaacta cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc
     1801 atagttacga gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt
     1861 gggttttact gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct
     1921 tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat cttgatatac
     1981 ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct
     2041 atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc
     2101 tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa gtactctatc ggactcgata
     2161 tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga
     2221 agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc
     2281 tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa
     2341 ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg
     2401 ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata
     2461 agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga
     2521 agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc
     2581 tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg
     2641 agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga
     2701 cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta
     2761 tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg
     2821 gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta
     2881 acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct
     2941 acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc
     3001 tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg
     3061 agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg
     3121 atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt
     3181 tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag
     3241 agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg
     3301 tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc
     3361 ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc
     3421 cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga atcccttact
     3481 acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg
     3541 aaaccatcac cccttggaac ttcgaagagg tcaatatgct gtcaaaggca ggagaccggt
     3601 ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
     3661 aagtggcacc gagtcggtgc tttcttttct tttccttttc gttgcttgtt ttccccttct
     3721 gatgttgtgt gttgagttta ttgtttggtt tgctggtaga tccagtattt ggatcatttt
     3781 tccttttttt tttctttcat tgcttctgcg gagggatgag tttgtgcatg aatcttttgt
     3841 tggttggatt cgcttagttt tcacactagt cctttgattc agcgcaaatc tttgttaatt
     3901 ggctcgctta attctgacat aaagtgttcc ttcttggttc tgcttgcagg tggtggataa
     3961 gggtgctagt gctcagtctt tcatcgagag gatgaccaac ttcgataaga accttccaaa
     4021 cgagaaggtg ctccctaagc actctttgct ctacgagtac ttcaccgtgt acaacgagtt
     4081 gaccaaggtt aagtacgtga ccgagggaat gaggaagcct gcttttttgt caggtgagca
     4141 aaagaaggct atcgttgatc tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct
     4201 caaagaggat tacttcaaga aaatcgagtg cttcgattca gttgagattt ctggtgttga
     4261 ggataggttc aacgcatctc tcggaaccta ccacgatctc ctcaagatca ttaaggataa
     4321 ggatttcttg gataacgagg aaaacgagga tatcttggag gatatcgttc ttaccctcac
     4381 cctctttgaa gatagagaga tgattgaaga aaggctcaag acctacgctc atctcttcga
     4441 tgataaggtg atgaagcagt tgaagagaag aagatacact ggttggggaa ggctctcaag
     4501 aaagctcatt aacggaatca gggataagca gtctggaaag acaatccttg atttcctcaa
     4561 gtctgatgga ttcgctaaca gaaacttcat gcagctcatc cacgatgatt ctctcacctt
     4621 taaagaggat atccagaagg ctcaggtttc aggacagggt gatagtctcc atgagcatat
     4681 cgctaacctc gctggatctc ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt
     4741 ggatgagttg gtgaaggtga tgggaaggca taagcctgag aacatcgtga tcgaaatggc
     4801 tagagagaac cagaccactc agaagggaca gaagaactct agggaaagga tgaagaggat
     4861 cgaggaaggt atcaaagagc ttggatctca gatcctcaaa gagcaccctg ttgagaacac
     4921 tcagctccag aatgagaagc tctacctcta ctacctccag aacggaaggg atatgtatgt
     4981 ggatcaagag ttggatatca acaggctctc tgattacgat gttgatcata tcgtgccaca
     5041 gtcattcttg aaggatgatt ctatcgataa caaggtgctc accaggtctg ataagaacag
     5101 gggtaagagt gataacgtgc caagtgaaga ggttgtgaag aaaatgaaga actattggag
     5161 gcagctcctc aacgctaagc tcatcactca gagaaagttc gataacttga ctaaggctga
     5221 gaggggagga ctctctgaat tggataaggc aggattcatc aagaggcagc ttgtggaaac
     5281 caggcagatc actaagcacg ttgcacagat cctcgattct aggatgaaca ccaagtacga
     5341 tgagaacgat aagttgatca gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc
     5401 tgatttcaga aaggatttcc aattctacaa ggtgagggaa atcaacaact accaccacgc
     5461 tcacgatgct taccttaacg ctgttgttgg aaccgctctc atcaagaagt atcctaagct
     5521 cgagtcagag ttcgtgtacg gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa
     5581 gtctgagcaa gagatcggaa aggctaccgc taagtatttc ttctactcta acatcatgaa
     5641 tttcttcaag accgagatta ccctcgctaa cggtgagatc agaaagaggc cactcatcga
     5701 gacaaacggt gaaacaggtg agatcgtgtg ggataaggga agggatttcg ctaccgttag
     5761 aaaggtgctc tctatgccac aggtgaacat cgttaagaaa accgaggtgc agaccggtgg
     5821 attctctaaa gagtctatcc tccctaagag gaactctgat aagctcattg ctaggaagaa
     5881 ggattgggac cctaagaaat acggtggttt cgattctcct accgtggctt actctgttct
     5941 cgttgtggct aaggttgaga agggaaagag taagaagctc aagtctgtta aggaacttct
     6001 cggaatcact atcatggaaa ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc
     6061 taagggatac aaagaggtta agaaggatct catcatcaag ctcccaaagt actcactctt
     6121 cgaactcgag aacggtagaa agaggatgct cgcttctgct ggtgagcttc aaaagggaaa
     6181 cgagcttgct ctcccatcta agtacgttaa ctttctttac ctcgcttctc actacgagaa
     6241 gttgaaggga tctccagaag ataacgagca gaagcaactt ttcgttgagc agcacaagca
     6301 ctacttggat gagatcatcg agcagatctc tgagttctct aaaagggtga tcctcgctga
     6361 tgcaaacctc gataaggtgt tgtctgctta caacaagcac agagataagc ctatcaggga
     6421 acaggcagag aacatcatcc atctcttcac ccttaccaac ctcggtgctc ctgctgcttt
     6481 caagtacttc gatacaacca tcgataggaa gagatacacc tctaccaaag aagtgctcga
     6541 tgctaccctc atccatcagt ctatcactgg actctacgag actaggatcg atctctcaca
     6601 gctcggtggt gattcaaggg ctgatcctaa gaagaagagg aaggtttgac tcgagatatg
     6661 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     6721 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     6781 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     6841 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     6901 ataaggagct caaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     6961 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     7021 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
     7081 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
     7141 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
     7201 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
     7261 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
     7321 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
     7381 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
     7441 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
     7501 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
     7561 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
     7621 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
     7681 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
     7741 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
     7801 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
     7861 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
     7921 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
     7981 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
     8041 gcccgcgcac ggctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
     8101 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
     8161 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
     8221 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
     8281 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
     8341 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
     8401 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
     8461 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
     8521 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
     8581 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
     8641 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
     8701 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
     8761 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
     8821 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
     8881 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
     8941 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
     9001 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
     9061 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
     9121 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
     9181 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
     9241 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
     9301 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     9361 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     9421 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     9481 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     9541 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     9601 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     9661 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     9721 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     9781 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     9841 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     9901 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     9961 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
    10021 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
    10081 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
    10141 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
    10201 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
    10261 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    10321 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    10381 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc
    10441 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    10501 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    10561 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
    10621 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
    10681 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
    10741 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
    10801 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
    10861 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aaacggtcac agcttgtctg
    10921 taagcggatg ccgggagcag acaagcgcgt cagcgggtgt tggcgggtgt cggggcgcag
    10981 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
    11041 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11101 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11701 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    11761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11941 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat
    12001 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac
    12061 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca
    12121 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac
    12181 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc
    12241 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt
    12301 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct
    12361 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca
    12421 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca
    12481 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg
    12541 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc
    12601 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt
    12661 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg
    12721 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt
    12781 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac
    12841 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag
    12901 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga
    12961 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
    13021 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
    13081 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
    13141 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
    13201 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
    13261 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt
    13321 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
    13381 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat
    13441 ctgggaacta ctcacacatt attatggaga aaatagagct tgtcgatcgt taattaatca
    13501 cctccaagat cccggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
    13561 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
    13621 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
    13681 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
    13741 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
    13801 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
    13861 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
    13921 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
    13981 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
    14041 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
    14101 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
    14161 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
    14221 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
    14281 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
    14341 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
    14401 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggacac
    14461 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
    14521 catcctagga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
    14581 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
    14641 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
    14701 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
    14761 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
    14821 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
    14881 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
    14941 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac
    15001 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    15061 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    15121 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    15181 agctgggcaa tggaatccga ggaggtttcc
//
