LOCUS       sequence-309971-        9919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of miniTurbo-3XNLS (as control) tagged 
            with N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9919)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K
FEATURES             Location/Qualifiers
     source          1..9919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        63..167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(168..236,237..1028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1199..1787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1688..1707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1941..1958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2075..2096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2111..2116,2117..2134,2135..2141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2149..2165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2154..2176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2173..2189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2173..2189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2208..2228
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2210..2228
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2256..2482
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2483..2663
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2710..2835
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3328..3561
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3746..3790
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4057..4173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4207..4227
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4227..5438
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5275..5294
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5442..5462)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             5466..5734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5744..5785
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5783..5807
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5819..5845
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5849..5875
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5879..5905
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5912..6682
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6701..6721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6725..6745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6749..6769
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6780..6804)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6834..7422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6887..6907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7305..7316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7436..7935
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7471..7494)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7868..7887
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7956..8555
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7956..7975)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8452..8472
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8693..8926
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8998..9119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9035..9054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9089..9108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9159..9294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9314..9333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9315..9333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9343..9360)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9343..9359)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9352..9374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(9501..9919,1..37)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9588..9607)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9798..9819
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
       61 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
      901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
      961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1321 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1621 gaaaggcgga caggcatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1861 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     1921 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     1981 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2041 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2101 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2161 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2221 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     2281 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     2341 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     2401 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     2461 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     2521 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     2581 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     2641 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     2701 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     2761 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     2821 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     2881 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     2941 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     3001 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     3061 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     3121 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     3181 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     3241 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     3301 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     3361 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     3421 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     3481 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3541 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3601 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3661 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3721 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3781 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3841 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3901 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     3961 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     4021 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     4081 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     4141 attacaaaaa caaattacaa aattcaaaat tttatcgtcc agtgtggtgg aattctgcag
     4201 atatcacaac tttgtataga aaagttggcc tccgcgccgg gttttggcgc ctcccgcggg
     4261 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     4321 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     4381 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     4441 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     4501 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     4561 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     4621 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
     4681 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
     4741 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
     4801 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
     4861 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
     4921 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     4981 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     5041 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     5101 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     5161 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     5221 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     5281 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     5341 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     5401 ccgctaaatt ctggccgttt ttggcttttt tgttagacgg ccaacttttg tatacaaagt
     5461 ggcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     5521 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     5581 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     5641 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     5701 ttgtttcgtt ttctgttctg cgccgttaca gatcggcacc atgggtaagc ctatccctaa
     5761 ccctctcctc ggtctcgatt ctacaacttt gtacaaaaaa gcaggcttag ccaccatgta
     5821 cccgtatgat gttccggatt acgctggcta tccctacgac gtgcccgact atgccgggta
     5881 cccctatgac gtcccagact acgcagctag catcccgctg ctgaacgcta aacagattct
     5941 gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta
     6001 cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca
     6061 ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct
     6121 gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg
     6181 aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc
     6241 caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat
     6301 aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt
     6361 ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga
     6421 caggaatact ctggccgcta tgctgatccg agagctgcgg gcagccctgg aactgttcga
     6481 gcaggaaggc ctggctccat atctgtcacg gtgggagaag ctggataact tcatcaatag
     6541 acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa
     6601 acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat
     6661 cagtctgcgg tctgccgaaa aggaattcag cagggccgac cccaagaaga agaggaaggt
     6721 ggaccccaag aagaagagga aggtggaccc caagaagaag aggaaggtgt gactcgagaa
     6781 cccagctttc ttgtacaaag ttgtgatatc cagcacagtg gcggccgctc gacaatcaac
     6841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6961 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7021 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7081 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7141 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7201 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7261 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7321 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7381 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta
     7441 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     7501 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     7561 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     7621 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     7681 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     7741 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     7801 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     7861 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     7921 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     7981 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     8041 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     8101 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     8161 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     8221 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     8281 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     8341 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     8401 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8461 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8521 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     8581 cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg
     8641 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8701 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     8761 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8821 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8881 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     8941 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     9001 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9061 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9121 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9241 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     9301 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     9361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     9421 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9481 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9541 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9601 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9661 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9721 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9781 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9841 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9901 gctgatttaa caaaaattt
//
