LOCUS       sequence-309972-        9817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of miniTurbo-NES (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9817)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(90..158,159..950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1121..1709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1610..1629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1863..1880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1997..2018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2033..2038,2039..2056,2057..2063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2071..2087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2076..2098
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2095..2111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2095..2111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2130..2150
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2132..2150
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2178..2404
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2405..2585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2632..2757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3250..3483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3668..3712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3979..4095
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4129..4149
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4149..5360
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5197..5216
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5364..5384)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             5388..5656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5666..5707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5705..5729
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5732..5741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5741..5782
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5789..6559
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6566..6592
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(6600..6624)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6654..7242
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6707..6727)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7125..7136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7256..7755
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7291..7314)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7688..7707
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7776..8375
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7776..7795)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8272..8292
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8513..8746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8818..8939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8855..8874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8909..8928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8979..9114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9134..9153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9135..9153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9163..9180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9163..9179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9172..9194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9321..9776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9408..9427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9618..9639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(9802..9817,1..89)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
       61 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      121 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      181 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      241 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      301 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      361 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      421 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      481 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      541 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      601 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
      661 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      721 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      781 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
      901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
      961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1261 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1681 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1741 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1801 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1861 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     1921 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1981 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2041 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2101 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     2161 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     2221 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     2281 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     2341 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     2401 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2461 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2521 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2581 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     2641 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     2701 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     2761 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     2821 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     2881 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     2941 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     3001 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     3061 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     3121 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     3181 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     3241 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     3301 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     3361 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     3421 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     3481 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     3541 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     3601 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     3661 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     3721 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     3781 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     3841 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     3901 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     3961 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4021 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4081 aaaattcaaa attttatcgt ccagtgtggt ggaattctgc agatatcaca actttgtata
     4141 gaaaagttgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     4201 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     4261 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     4321 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     4381 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     4441 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     4501 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     4561 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     4621 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     4681 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     4741 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     4801 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     4861 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     4921 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     4981 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     5041 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     5101 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     5161 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     5221 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     5281 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     5341 ttttggcttt tttgttagac ggccaacttt tgtatacaaa gtggcgaggg gctcgcatct
     5401 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     5461 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     5521 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     5581 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     5641 tgcgccgtta cagatcggca ccatgggtaa gcctatccct aaccctctcc tcggtctcga
     5701 ttctacaact ttgtacaaaa aagcaggctt agccaccatg ggcaagccca tccccaaccc
     5761 cctgctgggc ctggacagca ccgctagcat cccgctgctg aacgctaaac agattctggg
     5821 acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct
     5881 gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc
     5941 agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag
     6001 tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat
     6061 tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa
     6121 tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac
     6181 aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga
     6241 ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag
     6301 gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac tgttcgagca
     6361 ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca tcaatagacc
     6421 cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca
     6481 gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag
     6541 tctgcggtct gccgaaaagc tgcagctgcc tcccctggag cgcctgaccc tggactaaaa
     6601 cccagctttc ttgtacaaag ttgtgatatc cagcacagtg gcggccgctc gacaatcaac
     6661 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6721 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6781 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6841 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6901 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6961 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7021 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7081 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7141 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7201 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta
     7261 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     7321 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     7381 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     7441 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     7501 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     7561 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     7621 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     7681 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     7741 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     7801 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7861 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     7921 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     7981 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     8041 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     8101 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     8161 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     8221 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8281 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8341 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     8401 cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg
     8461 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8521 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     8581 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8641 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8701 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     8761 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     8821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8941 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9001 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9061 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     9121 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     9181 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     9241 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9301 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9361 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9421 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcrggggct
     9481 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9541 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9601 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9661 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9721 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     9781 ggcacttttc ggggaaatgt gcgcggaacc cctattt
//
