LOCUS       sequence-309973-       10252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of V5-GSK3B (human) tagged with N-terminal
            V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10252)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-GSK3B_IDG-K
FEATURES             Location/Qualifiers
     source          1..10252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        74..178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(179..247,248..1039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(397..416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1210..1798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1699..1718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1952..1969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2086..2107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2122..2127,2128..2145,2146..2152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2160..2176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2165..2187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2184..2200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2184..2200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2219..2239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2221..2239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2267..2493
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2494..2674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2721..2846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3339..3572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3757..3801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4068..4184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4218..4238
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4238..5449
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5286..5305
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5453..5473)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             5477..5745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5755..5796
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5794..5818
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6641..6652
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(7124..7148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7178..7766
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7231..7251)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7649..7660)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7780..8279
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7815..7838)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8212..8231
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8300..8899
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8300..8319)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8796..8816
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9037..9270
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9342..9463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9379..9398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9433..9452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9503..9638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9658..9677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9659..9677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9687..9704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9687..9703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9696..9718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(9845..10252,1..48)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9932..9951)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10142..10163
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
       61 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      121 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      181 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      241 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      301 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      361 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      421 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      481 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      541 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      601 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      661 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      721 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      781 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      841 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      901 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      961 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1021 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1081 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1141 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1261 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1321 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1381 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1441 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1501 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1561 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1621 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1681 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1741 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1801 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1861 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1921 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1981 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2041 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2101 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2161 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     2221 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     2281 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     2341 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     2401 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     2461 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     2521 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2581 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     2641 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     2701 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     2761 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     2821 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     2881 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     2941 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     3001 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     3061 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     3121 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     3181 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     3241 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     3301 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     3361 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     3421 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     3481 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     3541 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     3601 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     3661 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     3721 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     3781 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     3841 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     3901 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     3961 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     4021 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     4081 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4141 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg
     4201 gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg ggttttggcg
     4261 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     4321 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     4381 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     4441 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     4501 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     4561 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     4621 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     4681 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     4741 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     4801 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     4861 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     4921 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     4981 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     5041 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     5101 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     5161 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     5221 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     5281 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     5341 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     5401 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg gccaactttt
     5461 gtatacaaag tggcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     5521 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     5581 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     5641 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     5701 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatcggcac catgggtaag
     5761 cctatcccta accctctcct cggtctcgat tctacaactt tgtacaaaaa agttggcgcc
     5821 accatgtcag ggcggcccag aaccacctcc tttgcggaga gctgcaagcc ggtgcagcag
     5881 ccttcagctt ttggcagcat gaaagttagc agagacaagg acggcagcaa ggtgacaaca
     5941 gtggtggcaa ctcctgggca gggtccagac aggccacaag aagtcagcta tacagacact
     6001 aaagtgattg gaaatggatc atttggtgtg gtatatcaag ccaaactttg tgattcagga
     6061 gaactggtcg ccatcaagaa agtattgcag gacaagagat ttaagaatcg agagctccag
     6121 atcatgagaa agctagatca ctgtaacata gtccgattgc gttatttctt ctactccagt
     6181 ggtgagaaga aagatgaggt ctatcttaat ctggtgctgg actatgttcc ggaaacagta
     6241 tacagagttg ccagacacta tagtcgagcc aaacagacgc tccctgtgat ttatgtcaag
     6301 ttgtatatgt atcagctgtt ccgaagttta gcctatatcc attcctttgg aatctgccat
     6361 cgggatatta aaccgcagaa cctcttgttg gatcctgata ctgctgtatt aaaactctgt
     6421 gactttggaa gtgcaaagca gctggtccga ggagaaccca atgtttcgta tatctgttct
     6481 cggtactata gggcaccaga gttgatcttt ggagccactg attatacctc tagtatagat
     6541 gtatggtctg ctggctgtgt gttggctgag ctgttactag gacaaccaat atttccaggg
     6601 gatagtggtg tggatcagtt ggtagaaata atcaaggtcc tgggaactcc aacaagggag
     6661 caaatcagag aaatgaaccc aaactacaca gaatttaaat tccctcaaat taaggcacat
     6721 ccttggacta aggattcgtc aggaacagga catttcacct caggagtgcg ggtcttccga
     6781 ccccgaactc caccggaggc aattgcactg tgtagccgtc tgctggagta tacaccaact
     6841 gcccgactaa caccactgga agcttgtgca cattcatttt ttgatgaatt acgggaccca
     6901 aatgtcaaac taccaaatgg gcgagacaca cctgcactct tcaacttcac cactcaagaa
     6961 ctgtcaagta atccacctct ggctaccatc cttattcctc ctcatgctcg gattcaagca
     7021 gctgcttcaa cccccacaaa tgccacagca gcgtcagatg ctaatactgg agaccgtgga
     7081 cagaccaata atgctgcttc tgcatcagct tccaactcca cctgaccaac tttcttgtac
     7141 aaagttgtga tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat
     7201 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7261 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7321 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7381 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7441 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7501 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7561 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7621 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7681 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7741 gatctccctt tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc
     7801 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     7861 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     7921 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     7981 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     8041 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     8101 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     8161 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     8221 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     8281 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8341 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8401 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8461 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     8521 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8581 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8641 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8701 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8761 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8821 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8881 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     8941 acgaccccat gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg
     9001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac
     9061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9241 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     9301 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     9361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     9481 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9601 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     9661 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     9721 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     9781 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9841 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9901 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9961 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    10021 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    10081 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10141 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10201 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat tt
//
