LOCUS       sequence-309974-       10895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CAMK1G (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10895)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CAMK1G_IDG-K
FEATURES             Location/Qualifiers
     source          1..10895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(127..195,196..987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1158..1746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1647..1666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1900..1917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2034..2055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2070..2075,2076..2093,2094..2100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2108..2124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2113..2135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2132..2148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2132..2148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2167..2187
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2169..2187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2215..2441
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2442..2622
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2669..2794
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3287..3520
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3705..3749
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4016..4132
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5234..5253
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5401..5421)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5422..5431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5434..6204
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6211..6252
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6253..6277
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7715..7739)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7769..8357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7822..7842)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8240..8251)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8371..8870
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8406..8429)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8803..8822
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8891..9490
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8891..8910)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9387..9407
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9628..9861
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9933..10054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9970..9989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10024..10043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10094..10229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10249..10268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10250..10268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10278..10295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10278..10294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10287..10309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10436..10891
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10523..10542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10733..10754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
       61 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      121 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      181 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      241 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      301 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      361 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      421 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      481 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      541 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      601 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      661 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      721 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      781 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      841 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      901 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      961 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1021 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1081 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1801 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1861 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1921 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1981 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2041 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2101 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2161 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     2221 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     2281 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     2341 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     2401 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     2461 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2521 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2581 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2641 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2701 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2761 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2821 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2881 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     2941 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     3001 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     3061 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     3121 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3181 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3241 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3301 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3361 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3421 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3481 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3541 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3601 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3661 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3721 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3781 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3841 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3901 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3961 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     4021 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     4081 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgtcca
     4141 gtgtggtgga attctgcaga tatcacaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacggc
     5401 caacttttgt atacaaagtg ggccaccatg ggaatcccgc tgctgaacgc taaacagatt
     5461 ctgggacagc tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag
     5521 tacctgctgg atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag
     5581 caggcaggga gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac
     5641 ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc
     5701 ggaattgtca tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg
     5761 cccaatgacc tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga
     5821 ataacaggcg atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc
     5881 gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg
     5941 gacaggaata ctctggccgc tatgctgatc cgagagctgc gggcagccct ggaactgttc
     6001 gagcaggaag gcctggctcc atatctgtca cggtgggaga agctggataa cttcatcaat
     6061 agacccgtga agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat
     6121 aaacagggag ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa
     6181 atcagtctgc ggtctgccga aaaggctagc ggcaagccca tccccaaccc cctgctgggc
     6241 ctggacagca ccacaacttt gtacaaaaaa gcaggctcca ccatgggtcg aaaggaagaa
     6301 gatgactgca gttcctggaa gaaacagacc accaacatcc ggaaaacctt catttttatg
     6361 gaagtgctgg gatcaggagc tttctcagaa gttttcctgg tgaagcaaag actgactggg
     6421 aagctctttg ctctgaagtg catcaagaag tcacctgcct tccgggacag cagcctggag
     6481 aatgagattg ctgtgttgaa aaagatcaag catgaaaaca ttgtgaccct ggaggacatc
     6541 tatgagagca ccacccacta ctacctggtc atgcagcttg tttctggtgg ggagctcttt
     6601 gaccggatcc tggagcgggg tgtctacaca gagaaggatg ccagtctggt gatccagcag
     6661 gtcttgtcgg cagtgaaata cctacatgag aatggcatcg tccacagaga cttaaagccc
     6721 gaaaacctgc tttaccttac ccctgaagag aactctaaga tcatgatcac tgactttggt
     6781 ctgtccaaga tggaacagaa tggcatcatg tccactgcct gtgggacccc aggctacgtg
     6841 gctccagaag tgctggccca gaaaccctac agcaaggctg tggattgctg gtccatcggc
     6901 gtcatcacct acatattgct ctgtggatac cccccgttct atgaagaaac ggagtctaag
     6961 cttttcgaga agatcaagga gggctactat gagtttgagt ctccattctg ggatgacatt
     7021 tctgagtcag ccaaggactt tatttgccac ttgcttgaga aggatccgaa cgagcggtac
     7081 acctgtgaga aggccttgag tcatccctgg attgacggaa acacagccct ccaccgggac
     7141 atctacccat cagtcagcct ccagatccag aagaactttg ctaagagcaa gtggaggcaa
     7201 gccttcaacg cagcagctgt ggtgcaccac atgaggaagc tacacatgaa cctgcacagc
     7261 ccgggcgtcc gcccagaggt ggagaacagg ccgcctgaaa ctcaagcctc agaaacctct
     7321 agacccagct cccctgagat caccatcacc gaggcacctg tcctggacca cagtgtagca
     7381 ctccctgccc tgacccaatt accctgccag catggccgcc ggcccactgc ccctggtggc
     7441 aggtccctca actgcctggt caatggctcc ctccacatca gcagcagcct ggtgcccatg
     7501 catcaggggt ccctggccgc cgggccctgt ggctgctgct ccagctgcct gaacattggg
     7561 agcaaaggaa agtcctccta ctgctctgag cccacactcc tcaaaaaggc caacaaaaaa
     7621 cagaacttca agtcggaggt catggtacca gttaaagcca gtggcagctc ccactgccgg
     7681 gcagggcaga ctggagtctg tctcattatg taggacccag ctttcttgta caaagttgtg
     7741 atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7801 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7861 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7921 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7981 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8041 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8101 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8161 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8221 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8281 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8341 ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     8401 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     8461 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     8521 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     8581 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     8641 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     8701 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     8761 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     8821 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     8881 caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     8941 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9001 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9061 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     9121 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9181 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9241 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9301 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9361 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9421 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9481 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     9541 tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct gtagatctta
     9601 gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag
     9661 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9721 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9781 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9841 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     9901 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    10081 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10141 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10201 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    10261 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10321 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    10441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10861 aattttaaca aaatattaac gcttacaatt taggt
//
