LOCUS       sequence-309975-       10955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK16 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK16_IDG-K
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        81..185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(186..254,255..1046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1217..1805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1706..1725
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1959..1976
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2093..2114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2129..2134,2135..2152,2153..2159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2167..2183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2172..2194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2191..2207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2191..2207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2226..2246
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2228..2246
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2274..2500
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2501..2681
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2728..2853
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3346..3579
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3764..3808
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4075..4191
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4225..4245
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4245..5456
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5293..5312
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5460..5480)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5481..5490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5493..6263
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6270..6311
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6312..6336
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7834..7858)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7888..8476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7941..7961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8359..8370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8490..8989
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8525..8548)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8922..8941
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9010..9609
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9010..9029)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9506..9526
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9747..9980
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10052..10173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10089..10108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10143..10162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10213..10348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10368..10387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10369..10387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10397..10414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10397..10413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10406..10428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(10555..10955,1..55)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10642..10661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10852..10873
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
       61 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2041 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2101 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2161 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2221 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     2281 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     2341 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     2401 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     2461 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     2521 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2581 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2641 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2701 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2761 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2821 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2881 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2941 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     3001 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     3061 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     3121 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     3181 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     3241 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     3301 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     3361 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     3421 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     3481 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     3541 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     3601 aaaactcatt tgcacmactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     3661 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     3721 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     3781 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     3841 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     3901 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     3961 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     4021 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     4081 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     4141 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag
     4201 tgtggtggaa ttctgcagat atcacaactt tgtatagaaa agttggcctc cgcgccgggt
     4261 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     4321 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     4381 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     4441 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     4501 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     4561 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     4621 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     4681 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     4741 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     4801 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     4861 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     4921 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     4981 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     5041 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     5101 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     5161 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     5221 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     5281 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     5341 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     5401 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggcc
     5461 aacttttgta tacaaagtgg gccaccatgg gaatcccgct gctgaacgct aaacagattc
     5521 tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt
     5581 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
     5641 aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
     5701 tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg
     5761 gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
     5821 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa
     5881 taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg
     5941 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
     6001 acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg
     6061 agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata
     6121 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata
     6181 aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa
     6241 tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat ccccaacccc ctgctgggcc
     6301 tggacagcac cacaactttg tacaaaaaag caggctccac catggatcgg atgaagaaga
     6361 tcaaacggca gctgtcaatg acactccgag gtggccgagg catagacaag accaatggtg
     6421 cccctgagca gataggcctg gatgagagtg gtggtggtgg cggcagtgac cctggagagg
     6481 cccccacacg tgctgctcct ggggaacttc gttctgcacg gggcccactc agctctgcac
     6541 cagagattgt gcacgaggac ttgaagatgg ggtctgatgg ggagagtgac caggcttcag
     6601 ccacgtcctc ggatgaggtg cagtctccag tgagagtgcg tatgcgcaac catcccccac
     6661 gcaagatctc cactgaggac atcaacaagc gcctatcact accagctgac atccggctgc
     6721 ctgagggcta cctggagaag ctgaccctca atagccccat ctttgacaag cccctcagcc
     6781 gccgcctccg tcgtgtcagc ctatctgaga ttggctttgg gaaactggag acctacatta
     6841 agctggacaa actgggcgag ggtacctatg ccaccgtcta caaaggcaaa agcaagctca
     6901 cagacaacct tgtggcactc aaggagatca gactggaaca tgaagagggg gcaccctgca
     6961 ccgccatccg ggaagtgtcc ctactcaagg acctcaaaca cgccaacatc gttacgctac
     7021 atgacattat ccacacggag aagtccctca cccttgtctt tgagtacctg gacaaggacc
     7081 tgaagcagta cctggatgac tgtgggaaca tcatcaacat gcacaacgtg aaactgttcc
     7141 tgttccagct gctccgtggc ctggcctact gccaccggca gaaggtgcta caccgagacc
     7201 tcaagcccca gaacctgctc atcaacgaga ggggagagct caagctggct gactttggcc
     7261 tggcccgagc caagtcaatc ccaacaaaga catactccaa tgaggtggtg acactgtggt
     7321 accggccccc tgacatcctg cttgggtcca cggactactc cactcagatt gacatgtggg
     7381 gtgtgggctg catcttctat gagatggcca caggccgtcc cctctttccg ggctccacgg
     7441 tggaggaaca gctacacttc atcttccgta tcttaggaac cccaactgag gagacgtggc
     7501 caggcatcct gtccaacgag gagttcaaga catacaacta ccccaagtac cgagccgagg
     7561 cccttttgag ccacgcaccc cgacttgata gcgacggggc cgacctcctc accaagctgt
     7621 tgcagtttga gggtcgaaat cggatctccg cagaggatgc catgaaacat ccattcttcc
     7681 tcagtctggg ggagcggatc cacaaacttc ctgacactac ttccatattt gcactaaagg
     7741 agattcagct acaaaaggag gccagccttc ggtcttcgtc gatgcctgac tcaggcaggc
     7801 cagctttccg cgtggtggac accgagttct aggacccagc tttcttgtac aaagttgtga
     7861 tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga
     7921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8281 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8401 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8461 tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     8521 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     8581 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     8641 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     8701 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     8761 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     8821 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     8881 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     8941 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     9001 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     9061 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     9121 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     9181 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     9241 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     9301 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     9361 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     9421 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     9481 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     9541 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     9601 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     9661 gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9721 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
     9781 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9961 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
    10021 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
    10081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
    10201 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10261 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10321 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
    10381 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
    10441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
    10561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    10621 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    10681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    10741 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    10801 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    10861 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    10921 ttttgccgat ttcggcctat tggttaaaaa atgag
//
