LOCUS       sequence-309985-        9575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Kyriakakis P, Fernandez de Cossio L, Howard PW, Kouv S, Catanho M, 
            Hu VJ, Kyriakakis R, Allen ME, Ma Y, Aguilar-Rivera M, Coleman TP
  TITLE     Building a Simple and Versatile Illumination System for Optogenetic 
            Experiments.
  JOURNAL   J Vis Exp. 2021 Jan 12;(167). doi: 10.3791/61914.
  PUBMED    33522514
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2021-01-12"; volume: "(167). doi"; pages: " 
            10.3791/61914"
COMMENT     Sequence Label: pPK-352
FEATURES             Location/Qualifiers
     source          1..9575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             719..793
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2186..2260
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3008..3064
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3065..3139
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3161..3170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3170..3211
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3521..3577
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3578..3652
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3683..3700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(4909..4927)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4910..4927)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4947..4964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4953..5177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5223..5651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5310..5329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5520..5541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5660..5680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5665..5994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5845..5980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6061..6855
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6115..6134)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6725..6744
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7029..7150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7066..7085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7120..7139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7199..7215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7199..7215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7212..7234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7223..7239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7247..7253,7254..7271,7272..7277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7292..7313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7430..7447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7601..8189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7681..7700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8360..9151,9152..9220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8983..9002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9221..9325)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9400..9419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      601 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      661 gggagaccca agcttggtac cgagctcgga tcgctagttc gaaggatccg ccgccaccat
      721 gtccgtgctg accccactcc tgcttagggg ccttaccggc tctgccaggc gcctcccggt
      781 cccacgggca aagatccact cattgatgtc tctgagacag caccagcacc ccctgatcca
      841 gagactggcc gacagaatcg aggccatctg gcaggccttt ttccccctgg ccccttacgc
      901 cctgcctgag gatctgggct acgtggaagg caagctggaa ggcgagcggc tgaccatcga
      961 gaaccactgc taccaggccc ctccattccg gaagctgcac ctggaactgg ccagagtggg
     1021 agagtccctg gacatcctgc actgcgtgat gttccccgag cccagatacg acctgcccat
     1081 gttcggctgc gacctcgtgg gaggcagagg ccagatctct gccgccatcg tggatctgtc
     1141 tcccgtgacc ggacagctgc ctgccgctta cacctgtgcc ctgaatgccc tgcccaagct
     1201 gaccttccgg cagcccagag aactgcctcc ttggggccac atcttcagcc ctttctgcat
     1261 cttcatccgg ccccagggcg aggccgagga acagcagttc ctggatcgga tcggcgagta
     1321 cctgaccctg cactgtcagc tgtcccagca ggccgtgcct accgatcatc cacaggctgt
     1381 gatcgccggc cagagacagt actgccaaca gcagcagcag aacgacaaga ccagacgggt
     1441 gctggaaaag gccttcggcg tgccatgggc cgagcggtat atgaccaccg tgctgttcga
     1501 cgtgcccccc gtgtgaaagc ttgatatcca gcacagtggc ggccgcaagc ttaaaacagc
     1561 tctggggttg tacccacccc agaggcccac gtggcggcta gtactccggt attgcggtac
     1621 ccttgtacgc ctgttttata ctcccttccc gtaacttaga cgcacaaaac caagttcaat
     1681 agaagggggt acaaaccagt accaccacga acaagcactt ctgtttcccc ggtgatgtcg
     1741 tatagactgc ttgcgtggtt gaaagcgacg gatccgttat ccgcttatgt acttcgagaa
     1801 gcccagtacc acctcggaat cttcgatgcg ttgcgctcag cactcaaccc cagagtgtag
     1861 cttaggctga tgagtctgga catccctcac cggtgacggt ggtccaggct gcgttggcgg
     1921 cctacctatg gctaacgcca tgggacgcta gttgtgaaca aggtgtgaag agcctattga
     1981 gctacataag aatcctccgg cccctgaatg cggctaatcc caacctcgga gcaggtggtc
     2041 acaaaccagt gattggcctg tcgtaacgcg caagtccgtg gcggaaccga ctactttggg
     2101 tgtccgtgtt tccttttatt ttattgtggc tgcttatggt gacaatcaca gattgttatc
     2161 ataaagcgaa ttggaattcg ccaccatgtc cgtcctgacg ccgctgctgc tgcggggctt
     2221 gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tgggtaccgc
     2281 caccatgaca acatctctgg ccaccaagct gagagagggc accaagaaag cccacaccat
     2341 ggccgagaac gtgggcttcg tgcggtgctt cctgaaggga accgtggaaa agtcctccta
     2401 ccggaagctg gtggcctccc tgtaccacgt gtactccgcc atggaacagg aaatggaacg
     2461 gctgaaggac caccccatcg tgggcaagat ctacttcccc gagctgaacc ggaagtcctc
     2521 cctggaacgg gacctgacct actacttcgg ctccaactgg cgggaagaga tccccccatc
     2581 tcctgccacc caggcctacg tggccagaat ccacgaggtg gccaataccg cccctgagct
     2641 gctggtggct cactcctaca ccagatacct gggcgacctg tctggcggcc agattctgaa
     2701 gggcattgcc gagcgggcca tgaacctgca ggatggcgag ggcaccgcct tctacagatt
     2761 cgagtccatc tccgacgaga aggcctttaa gcagctgtac cggcagcggc tggacgagct
     2821 gcctgtggat gaggccaccg ccgacagaat cgtggacgag gccaatgccg ccttcggcat
     2881 gaacatgaag atctttcagg aactggaagg caacctgatc cgggccatcg gccagctgct
     2941 gttcaacacc ctgacccggc ggaagcagag aggctctacc gagctggcca ccgctgacgg
     3001 aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg
     3061 acctatgagc gtgctgacgc ccttgctgct tagaggactg accggaagcg cacgccgcct
     3121 gcccgtgcct agggcgaaga tacatagcct gttcgaagcc gccaccatgg gtaagcccat
     3181 tcccaacccg ctgttgggcc tcgatagcac agttaacatg gctacctaca aagtgacact
     3241 ggttcggcct gatggtagcg agacaacaat tgacgtgcct gaggacgaat acatactcga
     3301 cgttgcggaa gagcaagggc tggatctgcc atttagttgc cgagcgggcg cttgcagcac
     3361 ttgtgccggg aagttgctcg aaggggaagt ggatcagtct gatcagtcct ttctggatga
     3421 cgatcagatc gaaaaagggt tcgtgctcac ctgtgtcgct tatccaagga gcgattgtaa
     3481 gattctcact aatcaggagg aggagcttta cggctcaggc gcgacaaatt tttctctcct
     3541 caaacaggct ggggacgtgg aggagaaccc tggccctatg tccgttctta ccccactgtt
     3601 gttgcgggga ctcactggat cggctagacg actccccgtc ccaagggcaa agatccattc
     3661 tctggaattc gccgccacca tgcaccacca ccatcaccac gttaacatgt acaatgcaac
     3721 caactccagg agcagaatgt ttagatatga agtagtagga ctgaggcaga ctgccgaaac
     3781 cgaaaagacc aactatgcca tacgaaatag cggaagccag ttttttaacg taccctatga
     3841 taggatgaat cagttcatgc agcagataac acgctggggc ggaaagatcg tatccattca
     3901 gcccttgaac ggcacagtgg ctcccctggc tgccaccacg gaacccgctg caaataatgg
     3961 cgctgcgccc gtcaaagaga aaaaggttga tataccagtt aacatctatc gcccgaacaa
     4021 cccctgtatt ggaaaagtaa tatccaacga ggaactggtg agggagggcg gtgagggaac
     4081 cgtgaaacac attatttttg atatctctgg aaccgagctc agatatttgg agggccagtc
     4141 tatcgggatc attccggctg ggaccgacgc aaatggtaaa ccacataaac tccggctgta
     4201 ttctatcgcc tcaacgaggc acggggactt tcaggatgac aagaccgtct cactgtgcgt
     4261 tagacgcctc gaatataaag ataaagagac tggcgagacc atttatggcg tgtgctcctc
     4321 ctacctgaat cagctgcagc ctggagacga agtgaagatt actggtcccg ttgggaagga
     4381 aatgctgttg agtgacgacc ctgaagccac catcataatg cttgctaccg gcaccggaat
     4441 tgcccccttt cgcgctttct tgtggcggat gttcaaagaa aacaacccag attatcagtt
     4501 caaaggactt gcctggctgt tttttggcgt cgcctacacc gctaacatcc tgtataaaga
     4561 tgagctggag gccatccagg cccagtatcc tgatcacttt cggcttacat acgcaatttc
     4621 acgagagcag aagactccgg acggtgggaa aatgtacatc caggggcgaa tcgccgaaca
     4681 tgctgacgag atttggcagt tgcttcagaa aaaaaacaca cacgtctata tgtgcggcct
     4741 gaggggcatg gaacctggga tagatgaagc aatgacagca gccgccgcca agaatggcgc
     4801 agattggcag gaatttctta aaggaacatt gaagaaagag ggacggtggc acgtggaaac
     4861 atattaataa ccagcacagt ggtcgagcat gcatctagag ggccctattc tatagtgtca
     4921 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4981 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5041 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5101 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5161 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5221 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5281 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5341 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5401 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5461 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5521 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5581 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5641 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5701 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5761 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5821 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5881 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5941 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6001 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     6061 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6121 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6181 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6241 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6301 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6361 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6421 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6481 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6541 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6601 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6661 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6721 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6781 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6841 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6901 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6961 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7021 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7141 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7201 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7261 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7321 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9361 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9421 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     9481 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     9541 ggttaggcgt tttgcgctgc ttcgcgatgt acggg
//
