LOCUS       sequence-309996-       15184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with RcCat intron, RZ structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL086
FEATURES             Location/Qualifiers
     source          1..15184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2006
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3535..3610
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4103..6487
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6500..6520
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6531..6779
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6800..6824
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8125..8754
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9183..10256
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10322..10516
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10752..10774)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10850..10990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10905..10924
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11005..11022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11176..11764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11256..11275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11851..12645)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13070..13094
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13172..13346
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14436..15112)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14479..14499)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt
       61 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
      121 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
      181 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
      241 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
      301 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
      361 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
      421 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
      481 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
      541 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
      601 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
      661 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
      721 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
      781 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
      841 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
      901 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc
      961 ccaaatccac ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     1021 ctctctacct tctcaagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     1081 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctactagc gttcgtacac
     1141 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     1201 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     1261 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     1321 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     1381 cggtcgttca agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     1441 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     1501 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     1561 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     1621 caagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     1681 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     1741 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     1801 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     1861 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     1921 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     1981 cctgttgttt ggtgttactt ctgcagcctg caggtagatc gctcgtcgac atggataaga
     2041 agtactctat cggactcgat atcggaacta actctgtggg atgggctgtg atcaccgatg
     2101 agtacaaggt gccatctaag aagttcaagg ttctcggaaa caccgatagg cactctatca
     2161 agaaaaacct tatcggtgct ctcctcttcg attctggtga aactgctgag gctaccagac
     2221 tcaagagaac cgctagaaga aggtacacca gaagaaagaa caggatctgc tacctccaag
     2281 agatcttctc taacgagatg gctaaagtgg atgattcatt cttccacagg ctcgaagagt
     2341 cattcctcgt ggaagaagat aagaagcacg agaggcaccc tatcttcgga aacatcgttg
     2401 atgaggtggc ataccacgag aagtacccta ctatctacca cctcagaaag aagctcgttg
     2461 attctactga taaggctgat ctcaggctca tctacctcgc tctcgctcac atgatcaagt
     2521 tcagaggaca cttcctcatc gagggtgatc tcaaccctga taactctgat gtggataagt
     2581 tgttcatcca gctcgtgcag acctacaacc agcttttcga agagaaccct atcaacgctt
     2641 caggtgtgga tgctaaggct atcctctctg ctaggctctc taagtcaaga aggcttgaga
     2701 acctcattgc tcagctccct ggtgagaaga agaacggact tttcggaaac ttgatcgctc
     2761 tctctctcgg actcacccct aacttcaagt ctaacttcga tctcgctgag gatgcaaagc
     2821 tccagctctc aaaggatacc tacgatgatg atctcgataa cctcctcgct cagatcggag
     2881 atcagtacgc tgatttgttc ctcgctgcta agaacctctc tgatgctatc ctcctcagtg
     2941 atatcctcag agtgaacacc gagatcacca aggctccact ctcagcttct atgatcaaga
     3001 gatacgatga gcaccaccag gatctcacac ttctcaaggc tcttgttaga cagcagctcc
     3061 cagagaagta caaagagatt ttcttcgatc agtctaagaa cggatacgct ggttacatcg
     3121 atggtggtgc atctcaagaa gagttctaca agttcatcaa gcctatcctc gagaagatgg
     3181 atggaaccga ggaactcctc gtgaagctca atagagagga tcttctcaga aagcagagga
     3241 ccttcgataa cggatctatc cctcatcaga tccacctcgg agagttgcac gctatcctta
     3301 gaaggcaaga ggatttctac ccattcctca aggataacag ggaaaagatt gagaagattc
     3361 tcaccttcag aatcccttac tacgtgggac ctctcgctag aggaaactca agattcgctt
     3421 ggatgaccag aaagtctgag gaaaccatca ccccttggaa cttcgaagag gtaaatttct
     3481 agtttttctc cttcattttc ttggcttttg tcaccggagg agaccttggt ctcagtttta
     3541 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     3601 gagtcggtgc ttttgtcacc ggatgtgttt tccggtctga tgagtccgtg aggacgaaac
     3661 aaaagcgtta ggaccctttt ctctttttat ttttttgagc tttgatcttt ctttaaactg
     3721 atctattttt taattgattg gttatggtgt aaatattaca tagctttaac tgataatctg
     3781 attactttat ttcgtgtgtc tatgatgatg atgatagtta caggtggtgg ataagggtgc
     3841 tagtgctcag tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa
     3901 ggtgctccct aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa
     3961 ggttaagtac gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa
     4021 ggctatcgtt gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga
     4081 ggattacttc aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag
     4141 gttcaacgca tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt
     4201 cttggataac gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt
     4261 tgaagataga gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa
     4321 ggtgatgaag cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct
     4381 cattaacgga atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga
     4441 tggattcgct aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga
     4501 ggatatccag aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa
     4561 cctcgctgga tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga
     4621 gttggtgaag gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga
     4681 gaaccagacc actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga
     4741 aggtatcaaa gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct
     4801 ccagaatgag aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca
     4861 agagttggat atcaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcatt
     4921 cttgaaggat gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa
     4981 gagtgataac gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct
     5041 cctcaacgct aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg
     5101 aggactctct gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca
     5161 gatcactaag cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa
     5221 cgataagttg atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt
     5281 cagaaaggat ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga
     5341 tgcttacctt aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc
     5401 agagttcgtg tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga
     5461 gcaagagatc ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt
     5521 caagaccgag attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa
     5581 cggtgaaaca ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt
     5641 gctctctatg ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc
     5701 taaagagtct atcctcccta agaggaactc tgataagctc attgctagga agaaggattg
     5761 ggaccctaag aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt
     5821 ggctaaggtt gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat
     5881 cactatcatg gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg
     5941 atacaaagag gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact
     6001 cgagaacggt agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct
     6061 tgctctccca tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa
     6121 gggatctcca gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt
     6181 ggatgagatc atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa
     6241 cctcgataag gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc
     6301 agagaacatc atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta
     6361 cttcgataca accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac
     6421 cctcatccat cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg
     6481 tggtgattca agggctgatc ctaagaagaa gaggaaggtt tgactcgaga tatgaagatg
     6541 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     6601 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatcaca
     6661 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     6721 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagg
     6781 agctcaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     6841 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     6901 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     6961 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     7021 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     7081 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     7141 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     7201 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     7261 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     7321 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     7381 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     7441 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     7501 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     7561 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     7621 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     7681 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     7741 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     7801 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     7861 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     7921 gcacggctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     7981 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     8041 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     8101 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     8161 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     8221 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     8281 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     8341 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     8401 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     8461 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     8521 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     8581 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     8641 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     8701 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     8761 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     8821 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     8881 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     8941 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     9001 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     9061 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     9121 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     9181 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     9241 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     9301 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     9361 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     9421 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     9481 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     9541 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     9601 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     9661 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     9721 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     9781 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     9841 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     9901 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     9961 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
    10021 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
    10081 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    10141 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    10201 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    10261 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggcctctttc
    10321 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    10381 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    10441 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    10501 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
    10561 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    10621 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    10681 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    10741 gacggtgaaa acctctgaca catgcagctc ccggaaacgg tcacagcttg tctgtaagcg
    10801 gatgccggga gcagacaagc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
    10861 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
    10921 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10981 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11581 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    11641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11821 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
    11881 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    11941 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    12001 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    12061 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    12121 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    12181 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    12241 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    12301 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    12361 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    12421 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    12481 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    12541 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    12601 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    12661 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    12721 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    12781 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    12841 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    12901 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    12961 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    13021 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    13081 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    13141 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
    13201 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
    13261 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
    13321 actactcaca cattattatg gagaaaatag agcttgtcga tcgttaatta atcacctcca
    13381 agatcccgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
    13441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
    13501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
    13561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
    13621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
    13681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
    13741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
    13801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
    13861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
    13921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
    13981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
    14041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
    14101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
    14161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
    14221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
    14281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg acacgctgtc
    14341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatcct
    14401 aggagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat
    14461 gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt
    14521 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
    14581 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
    14641 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
    14701 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
    14761 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
    14821 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt
    14881 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
    14941 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
    15001 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
    15061 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
    15121 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
    15181 catg
//
