LOCUS       sequence-310-exp        4771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4771)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD152.79
FEATURES             Location/Qualifiers
     source          1..4771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1142..1170)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1918..1938
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2516..2620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2621..2689,2690..3481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2839..2858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3652..4240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4141..4160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4394..4411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4528..4549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4564..4569,4570..4587,4588..4594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4602..4618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4607..4629
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4626..4642
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4626..4642
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcagta ggtacaagga aactatttta ttgaattctt gatcaaagaa
       61 ggtaggcagt ttatataaat aatcttattg ttagatacca ttttgaatat atgtttcagt
      121 tccagaaaaa taagctatac atagtatcga tttggaacat atataacgaa aacagacgtc
      181 atgaaatcac acattggaaa agtgagaaag ttgttgagta gaggaggtga caaaagtgag
      241 atgccaactc acttatccat tctactcgtt ttgtgctctt ttccactttc tgatgtctgc
      301 tcatcttctt attcttcaag cgttttgtct attctcacac ctctccaatc tttcagtttc
      361 tgtttttaat gtctagacac gcgtgtaacg tacaacataa atgtttgacg tcaaccagat
      421 gtagtacaat aaaaaatata ttcagattac ctagaaaatt acttcggtca tgaccaccaa
      481 ttttttgatt tttcgatttt cgaaaccgac atcgttgaag aacattcagg tcataatttt
      541 ttatttgaaa aataatgttt tctatgtatt aattcggttt agtcgatcga atatgtacag
      601 ttgagaatta ctagagtacg gtgcgcaagt atgcaaactt gcgtaacggt gtttacacat
      661 ttgtcacgtt tctctgcgtc tcttcgtctc aacaatgctt gcactccgtc acattttcga
      721 attgtcggcc gatatcggta tttagataaa ttcagtgctc tccttttttt ctccatttta
      781 ctttcatcaa tcttttttaa aaatcaatat tcagcaaact attgaaaaat taattcaaaa
      841 aacacgactg gtcggaagtt atttgcataa tcaatagaaa tttcgttttc cgctcgctgt
      901 cagtgttttg tcctgtttgt atttcccagt gcacttttcg agtggctcaa gtttttatct
      961 attttcctgc aaacttgcat atattgtttt cttccagaag cctttgatat ctcaataatt
     1021 ttatacttgt ttcagaaatc gagcaaaaac caagatagct gaggatccta tcgattcgcg
     1081 gccgctgtac acccgggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
     1141 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1201 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1261 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
     1321 tttaaatttt cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag
     1381 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     1441 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
     1501 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
     1561 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1621 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1681 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga
     1741 tttaaatttt cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1801 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1861 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1921 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggacgaac tatacaaaag
     1981 cccacaagct ttggaattcg ctagccggcc atacaagtaa tccggatgat cgacgccaac
     2041 gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat tatttattta
     2101 ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt
     2161 tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc
     2221 aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg
     2281 gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa
     2341 ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa
     2401 actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttgggccc
     2461 aaaaggcctt ttttttttgg cgccgacgtc aggtggcact tttcggggaa atgtgcgcgg
     2521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2581 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2641 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2701 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2761 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2821 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2881 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2941 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3001 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3061 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3121 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3181 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3241 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3301 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3361 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3421 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3481 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3541 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3601 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3781 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4021 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     4081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4261 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4321 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4381 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     4441 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     4501 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     4561 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     4621 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
     4681 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
     4741 gatggatacg ctaacaactt ggaaatgaaa t
//
