LOCUS       sequence-310002-       14177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas12a guide RNA unit insertion vector with OsCDPK2 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL087
FEATURES             Location/Qualifiers
     source          1..14177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1211..1231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4953..5000
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5011..5259
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    5280..5304
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6605..7234
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7663..8736
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8802..8996
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9232..9254)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9330..9470
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9385..9404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9485..9502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9656..10244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9736..9755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10331..11125)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11550..11574
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11652..11826
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(12922..13267)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12959..12979)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        join(13376..14177,1..1196)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
ORIGIN
        1 tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc
       61 tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt
      121 cggagcgcac acacacacaa ccagaactcc cccaaatcca cccgtcggca cctccgcttc
      181 aaggtacgcc gctcgtcctc cccccccccc ctctctacct tctcaagatc ggcgttccgg
      241 tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt
      301 gttagatccg tgctactagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg
      361 attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca
      421 gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc
      481 ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg
      541 tcttggttgt gatgatgtgg tctggttggg cggtcgttca agatcggagt agaattaatt
      601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
      661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
      721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
      781 gtggtgtggt tgggcggtcg ttcattcgtt caagatcgga gtagaatact gtttcaaact
      841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
      901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
      961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     1021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     1081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     1141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcagcctg
     1201 caggatggct cctaagaaga agcggaaggt tggtattcac ggggtgcctg cggcttcaaa
     1261 gctcgagaaa ttcaccaact gttattcgtt gagcaaaaca ctgcggttta aagcgattcc
     1321 agtcggcaag actcaagaga atatagacaa taagcggctg ttggtggaag atgaaaagcg
     1381 cgcggaagac tacaaagggg tgaagaagtt gttggacaga tactacctct cttttatcaa
     1441 tgatgtcttg cactcaatca aattgaagaa tctgaacaac tacatctccc tcttcagaaa
     1501 gaaaacaagg acagaaaagg agaataagga acttgaaaat ttggagatca atctgaggaa
     1561 agagatcgcg aaagccttta aaggcaacga aggatacaaa agtctgttca agaaggatat
     1621 aattgagaca attttgccag agttcctcga tgacaaggac gagattgcgc tggtcaattc
     1681 gttcaacgga ttcacaacag cattcacagg cttctttgat aatcgggaaa atatgttctc
     1741 tgaggaggca aagtccactt ctattgcgtt caggtgtatc aatgagaatc tcactaggta
     1801 catttccaac atggatatct ttgagaaggt tgacgcaatt tttgacaagc acgaagttca
     1861 ggagattaag gagaagatcc tcaattccga ttatgacgtt gaggacttct tcgaaggtga
     1921 gttttttaat ttcgtgctca ctcaagaggg tatcgacgtg tataatgcga tcatcggtgg
     1981 gttcgtgact gagtccggtg aaaagattaa gggattgaac gagtatatca acctttacaa
     2041 ccaaaagacg aaacagaagc tgccaaagtt caagcctctt tacaaacagg ttctttcaga
     2101 ccgcgagtca ctctcgttct atggggaggg ctacacttcg gatgaggaag tcctggaggt
     2161 gttcaggaat actctcaata agaattcgga gattttctct tctataaaaa aactggaaaa
     2221 gttgtttaag aattttgacg aatactctag cgccggcata tttgtgaaaa acggcccggc
     2281 catatcaacg ataagtaaag atatcttcgg cgaatggaac gtgatcagag acaaatggaa
     2341 cgcggagtat gacgatattc acctgaagaa gaaggctgtc gtaacggaga agtacgagga
     2401 tgatcgcagg aaaagcttca aaaagatcgg aagtttcagc ctggaacagt tgcaggagta
     2461 tgctgacgcc gatcttagcg tcgtcgagaa gttgaaggag ataatcatcc aaaaggtcga
     2521 tgagatatat aaagtctatg gatcaagtga aaaactgttc gacgccgact tcgttttgga
     2581 gaagtccctg aagaagaacg acgctgttgt tgccattatg aaggatctgc tcgacagcgt
     2641 gaagagtttc gagaactata ttaaggcttt tttcggggag gggaaggaga ctaacagaga
     2701 tgagtccttc tacggagact tcgtcctcgc gtacgatata ctccttaagg tagaccacat
     2761 ctacgacgca atcagaaatt acgtgacaca aaagccgtac agcaaggaca agttcaaact
     2821 ctacttccag aacccccagt tcatgggcgg ctgggacaag gacaaggaaa cggattacag
     2881 ggctacgatc ctgaggtatg gttcaaaata ctacttggcg attatggaca agaagtacgc
     2941 caagtgtctc cagaagattg acaaagacga tgtcaatggc aattatgaga agatcaacta
     3001 caagctgctt ccgggtccga acaagatgct cccaaaggtt ttcttcagca agaaatggat
     3061 ggcctactat aacccaagcg aggacatcca gaagatttat aagaacggta cgttcaagaa
     3121 gggcgacatg ttcaatctta acgactgtca caagctgatc gacttcttca aagactcaat
     3181 tagccggtac ccaaagtggt ctaacgccta tgacttcaac ttttcggaaa ccgagaagta
     3241 caaggatata gccggatttt atagagaggt ggaagagcag ggctacaagg tgtcattcga
     3301 gtccgccagc aagaaggaag tggacaagct cgtggaagag ggtaagctct acatgttcca
     3361 gatttataat aaagacttta gcgataagag ccacgggaca cctaatctcc acacaatgta
     3421 tttcaagctg ctcttcgacg agaataacca cggccaaatc aggttgtcag gaggggctga
     3481 actcttcatg cggcgcgcat ccctgaagaa ggaggagctt gtagtccacc ctgcgaatag
     3541 tccaattgcg aataagaacc cggacaatcc taaaaagact acaacattga gctacgacgt
     3601 gtacaaggat aagaggtttt ccgaggatca gtacgagctc cacatcccga ttgcgatcaa
     3661 caagtgccca aagaatattt tcaagataaa cacagaggtg cgtgtactcc tgaagcatga
     3721 cgacaatcct tacgtcattg ggattgatcg gggcgagagg aacctcctct atattgtggt
     3781 ggtggacggg aaggggaaca tagtcgaaca gtactccctt aacgaaataa ttaacaattt
     3841 caacggcatc cgtatcaaga ccgactacca ttcgttgctg gacaagaagg agaaggagag
     3901 atttgaggcg cggcaaaatt ggacaagtat cgagaacatc aaggaactca aagcaggtta
     3961 tatctctcaa gttgtgcata agatatgcga gctggttgag aagtatgacg cagtgatcgc
     4021 tcttgaggac ctcaactcgg gctttaagaa tagccgcgtt aaagtggaga agcaggtcta
     4081 tcaaaagttc gagaagatgc ttatagataa gctcaactac atggtcgata agaaatcgaa
     4141 cccatgtgcc accggcggcg cactcaaagg ttaccaaata acaaacaaat tcgagtcctt
     4201 caaatcgatg agtactcaga atgggttcat attttatata ccggcgtggc ttacgtctaa
     4261 gatcgacccg tcaactggtt ttgtcaacct gttgaagacg aaatacacgt ccattgccga
     4321 ttcgaaaaag ttcatatcta gttttgatcg tattatgtac gtcccagagg aagatctttt
     4381 cgagtttgct ctcgactaca aaaacttttc gcggaccgat gcggattaca ttaaaaaatg
     4441 gaaactctat tcgtacggca acagaatcag gatttttcgc aaccctaaga agaataacgt
     4501 ctttgattgg gaggaagttt gcttgactag cgcgtacaag tgagaccccg gtctcagagc
     4561 tctttaataa gtatggcatt aactaccaac agggtgatat cagagcactg ctttgcgaac
     4621 aatctgacaa ggctttctac tcatccttca tggctttgat gagcctgatg ctccagatga
     4681 gaaattcaat tacaggcaga accgacgtgg atttcttgat ctccccggtt aaaaattctg
     4741 atggcatctt ttacgatagc aggaactatg aagcgcaaga gaatgcgatt ctgccaaaaa
     4801 atgcagacgc caacggtgcc tataacatcg ccaggaaagt cctgtgggcg atcggccagt
     4861 tcaaaaaggc cgaagacgaa aaattggaca aggtcaaaat cgctatcagc aacaaagagt
     4921 ggctggagta tgctcagaca tccgtaaagc ataagcgtcc tgctgccacc aaaaaggccg
     4981 gacaggctaa gaaaaagaag tgatctagaa tatgaagatg aagatgaaat atttggtgtg
     5041 tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa
     5101 tttgtggctt tttctaatat taaatgaatg taagatcaca ttataatgaa taaacaaatg
     5161 tttctataat ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat
     5221 gtgctatggt atggactatg gaatatgatt aaagataagg agctcaaact atcagtgttt
     5281 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     5341 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     5401 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     5461 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     5521 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     5581 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     5641 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     5701 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     5761 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     5821 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     5881 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     5941 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     6001 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     6061 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     6121 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     6181 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     6241 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     6301 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     6361 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacggctca accgtgcggc
     6421 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     6481 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     6541 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     6601 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     6661 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     6721 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     6781 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     6841 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     6901 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     6961 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     7021 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     7081 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     7141 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     7201 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     7261 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     7321 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     7381 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     7441 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     7501 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     7561 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     7621 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     7681 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     7741 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     7801 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     7861 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     7921 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     7981 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     8041 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     8101 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     8161 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     8221 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     8281 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     8341 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     8401 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     8461 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     8521 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     8581 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     8641 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     8701 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     8761 attgccctag caggggaaaa aggtcgaaaa ggcctctttc ctgtggatag cacgtacatt
     8821 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     8881 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     8941 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     9001 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     9061 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     9121 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     9181 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9241 catgcagctc ccggaaacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9301 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     9361 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9421 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     9481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10081 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
    10321 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    10381 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    10441 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    10501 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    10561 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    10621 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    10681 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    10741 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    10801 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    10861 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    10921 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    10981 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    11041 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    11101 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    11161 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    11221 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    11281 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    11341 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
    11401 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
    11461 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    11521 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
    11581 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
    11641 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
    11701 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
    11761 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
    11821 gagaaaatag agcttgtcga tcgttaatta atcacctcca agatcccgga cgagtgctgg
    11881 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
    11941 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
    12001 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
    12061 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
    12121 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
    12181 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
    12241 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
    12301 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
    12361 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
    12421 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
    12481 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
    12541 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
    12601 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
    12661 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
    12721 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
    12781 cttcgccctc cgagagctgc atcaggtcgg acacgctgtc gaacttttcg atcagaaact
    12841 tctcgacaga cgtcgcggtg agttcaggct ttttcatcct aggagagata gatttgtaga
    12901 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
    12961 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    13021 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    13081 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    13141 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    13201 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    13261 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    13321 agtgtcgtgc tccaccatgg gactagtagt cgtcagtcag tactagccgg cgcgcctgca
    13381 gtgcagcgtg acccggtcgt gcccctctct tgagataagg tgagcattgc atgtctaagt
    13441 tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct
    13501 ttatacatat atttaaactt tactctacga ataatataat ctatagtact acaataatat
    13561 cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga caattgagta
    13621 ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct cctttttttt
    13681 tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc atttagggtt
    13741 tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt ctattttagc
    13801 ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat ttagatataa
    13861 aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac
    13921 taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga
    13981 gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg
    14041 cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca ccgttggact
    14101 tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc
    14161 aggcggcctc ctcctcc
//
