LOCUS       sequence-310010-       11925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for doxycycline inducible expression of human Citron 
            shRNA, GFP marker, based on Addgene 11652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11925)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Dox human Citron shRNA 1 GFP
FEATURES             Location/Qualifiers
     source          1..11925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2184..2683
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2219..2242)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2616..2635
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2734..2743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2740..2742,2743..2745,2746..3456)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2740..2742,2743..2745,2746..3456)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2785..2806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3046..3065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3393..3414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3534..4101
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3552..4103
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3696..3713)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3923..3942
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4727..4747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4850..5044
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5183..5199)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5184..5200)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5220..5808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5273..5293)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5691..5702)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5976..5994
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6018..6036
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6060..6078
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6102..6120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6144..6162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6191..6405
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     6326..6345
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(6487..6520)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6540..6720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6745..6763)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6746..6763)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7053..7072)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7286..7305)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7405..7427
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7465..7483)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7551..7655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7656..7724,7725..8516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7874..7893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8687..9275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9176..9195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9429..9446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9516..9536)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9521..9850
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9701..9836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10984..11049
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11179..11199
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11624..11758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11661..11680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11715..11734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
     2161 agcctcgacg atggtcgagt accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     2221 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     2281 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     2341 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     2401 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     2461 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     2521 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     2581 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     2641 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcta gcgggatcca
     2701 ggcctaagct tacgcgtcct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
     2761 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2821 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2881 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2941 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3001 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3061 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3121 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3181 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3241 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3301 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3361 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3421 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgactagct
     3481 agtagctagc tagctagtcg agctcaagct tcgaattccc gggctgcagg aattccgncc
     3541 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     3601 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     3661 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     3721 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     3781 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     3841 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     3901 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     3961 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4021 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4081 ctttgaaaaa cacgatgata ataccatggc tagactggac aagagcaaag tcataaacgg
     4141 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     4201 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     4261 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     4321 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
     4381 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     4441 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     4501 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     4561 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     4621 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     4681 aatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcgccaa aaaagaagag
     4741 aaaggtcgac ggcggtggtg ctttgtctcc tcagcactct gctgtcactc aaggaagtat
     4801 catcaagaac aaggagggca tggatgctaa gtcactaact gcctggtccc ggacactggt
     4861 gaccttcaag gatgtatttg tggacttcac cagggaggag tggaagctgc tggacactgc
     4921 tcagcagatc gtgtacagaa atgtgatgct ggagaactat aagaacctgg tttccttggg
     4981 ttatcagctt actaagccag atgtgatcct ccggttggag aagggagaag agccctggct
     5041 ggtggagaga gaaattcacc aagagaccca tcctgattca gagactgcat ttgaaatcaa
     5101 atcatcagtt taaggatctc gactagctag tagctagcta gctagtcgag ctcaagcttc
     5161 gaattcgata tcaagcttat cgcgataccg tcgacctcga cctcgactag tcatatgata
     5221 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5281 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5341 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5401 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5461 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5521 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5581 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5641 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5701 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5761 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtacgtat
     5821 ggccaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5881 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat
     5941 tcaccatgct agtggatccc cctcgagttt accactccct atcagtgata gagaaaagtg
     6001 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     6061 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     6121 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcggggct
     6181 gcaggaattc gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc ccagtgtcac
     6241 taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag atggctgtga gggacagggg
     6301 agtggcgccc tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga
     6361 aatgtctttg gatttgggaa tcttataagt tctgtatgag accacgcgcc catggaaggc
     6421 actatttctc aattcaagag attgagaaat agtgccttcc attttttacg ataccgtcgc
     6481 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
     6541 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6601 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6661 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6721 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     6781 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     6841 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     6901 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     6961 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     7021 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     7081 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     7141 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     7201 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     7261 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     7321 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7381 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     7441 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     7501 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7561 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7621 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7741 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7801 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7861 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7981 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8041 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8101 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8161 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8521 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8581 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8821 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9061 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9481 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     9541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9601 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9661 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9721 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9841 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     9901 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     9961 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
    10021 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
    10081 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
    10141 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
    10201 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
    10261 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
    10321 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
    10381 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
    10441 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
    10501 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
    10561 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
    10621 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
    10681 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
    10741 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
    10801 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
    10861 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
    10921 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    10981 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    11041 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    11101 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    11161 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    11221 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    11281 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    11341 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    11401 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    11461 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    11521 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    11581 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    11641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    11761 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    11821 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    11881 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
